The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sprenger, H.
Right arrow Articles by Gemsa, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sprenger, H.
Right arrow Articles by Gemsa, D.

The Journal of Immunology, Vol 152, Issue 1 280-289, Copyright © 1994 by American Association of Immunologists

ARTICLES

Characterization of a high molecular weight tumor necrosis factor-alpha mRNA in influenza A virus-infected macrophages

H Sprenger, M Bacher, E Rischkowsky, A Bender, M Nain and D Gemsa
Institute of Immunology, Philipps University, Marburg, Germany.

Infection by influenza A virus has previously been shown to prime macrophages for a high TNF-alpha production. Influenza A virus induced a TNF-alpha mRNA accumulation that consisted of two types: a regular 1.7 kb and an additional high m.w. 2.4 kb species in murine macrophages, and a high m.w. 3.6 kb species in human monocytes. In this study, we further characterized this virus-induced, novel high m.w. TNF- alpha mRNA. The additional high m.w. TNF-alpha mRNA represented a true polyadenylated mRNA and its induction required exposure to infectious viruses. The regular and the high m.w. TNF-alpha mRNA were both found in the nuclear fraction and the cytoplasm. We excluded that the novel high m.w. TNF-alpha mRNA was an intron-containing precursor TNF-alpha mRNA that could have persisted in virus-infected macrophages. When TNF- alpha exons 1 to 4 and TNF-alpha exons 2 to 4 were amplified by polymerase chain reaction, only regular and no high m.w. bands were detected. By use of specific TNF-alpha intron I and intron III cDNA we could definitely demonstrate the absence of introns in the high m.w. TNF-alpha mRNA. The high m.w. TNF-alpha mRNA was free of TNF-beta and TNF intergenic region elements but contained the 5' and 3' untranslated region of TNF-alpha. Influenza A virus infection also induced a double band of IL-1 beta and IL-6 mRNA. Whether this novel high m.w. TNF-alpha mRNA represents a virus-induced abnormality or a superinduction of an otherwise normal but minor TNF-alpha transcript, and whether this high m.w. TNF-alpha mRNA species codes for a biologically active product, remains to be examined.


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
R. Salentin, D. Gemsa, H. Sprenger, and A. Kaufmann
Chemokine receptor expression and chemotactic responsiveness of human monocytes after influenza A virus infection
J. Leukoc. Biol., August 1, 2003; 74(2): 252 - 259.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. F. Rimmelzwaan, M. M. J. W. Baars, P. de Lijster, R. A. M. Fouchier, and A. D. M. E. Osterhaus
Inhibition of Influenza Virus Replication by Nitric Oxide
J. Virol., October 1, 1999; 73(10): 8880 - 8883.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. Rayet, J.-A. Lopez-Guerrero, J. Rommelaere, and C. Dinsart
Induction of Programmed Cell Death by Parvovirus H-1 in U937 Cells: Connection with the Tumor Necrosis Factor Alpha Signalling Pathway
J. Virol., November 1, 1998; 72(11): 8893 - 8903.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1994 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1994 by The American Association of Immunologists, Inc. All rights reserved.