The Journal of Immunology, Vol 151, Issue 9 4821-4828, Copyright © 1993 by American Association of Immunologists

ARTICLES

Killing of Staphylococcus aureus by tumor necrosis factor-alpha- activated neutrophils. The role of serum opsonins, integrin receptors, respiratory burst, and degranulation

A Ferrante, AJ Martin, EJ Bates, DH Goh, DP Harvey, D Parsons, DA Rathjen, G Russ and JM Dayer
Department of Immunology, Adelaide Children's Hospital, South Australia.

We have examined the effects of TNF priming on the killing of Staphylococcus aureus by human neutrophils. In the absence of serum opsonins, neutrophils failed to kill S. aureus, and TNF priming did not induce the cells to become bactericidal. Normal human serum, containing complement activity, promoted the killing of the bacteria by neutrophils. Pretreatment of neutrophils for 30 min with TNF significantly enhanced their bactericidal activity. The effects of TNF on neutrophil bactericidal activity was dependent on serum concentration and the degree of enhancement induced increased up to a concentration of 1%. The kinetics of bacterial killing showed that TNF- only enhanced the initial rate of killing, over the first 30 min. Little killing of bacteria occurred in the presence of complement- inactivated serum, and TNF did not stimulate this killing. These results suggest that TNF enhances the neutrophil complement-dependent killing of S. aureus. TNF increased the expression of CR3 (CD11b/CD18) and CR4 (P150, 95; CD11c/CD18) adhesion receptors but not LFA-1 (CD11a/CD18); and mAb against the alpha-chain of either CR3 or CR4 but not LFA-1 prevented the enhancing effects of TNF on the neutrophil bactericidal activity.

This article has been cited by other articles:

				M. von Kockritz-Blickwede, M. Rohde, S. Oehmcke, L. S. Miller, A. L. Cheung, H. Herwald, S. Foster, and E. Medina Immunological Mechanisms Underlying the Genetic Predisposition to Severe Staphylococcus aureus Infection in the Mouse Model Am. J. Pathol., December 1, 2008; 173(6): 1657 - 1668. [Abstract] [Full Text] [PDF]

				L. Shi, K. Takahashi, J. Dundee, S. Shahroor-Karni, S. Thiel, J. C. Jensenius, F. Gad, M. R. Hamblin, K. N. Sastry, and R. A. B. Ezekowitz Mannose-binding Lectin-deficient Mice Are Susceptible to Infection with Staphylococcus aureus J. Exp. Med., May 17, 2004; 199(10): 1379 - 1390. [Abstract] [Full Text] [PDF]

				H. F. Ismail, J. Zhang, R. G. Lynch, Y. Wang, and D. J. Berg Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice Infect. Immun., December 1, 2003; 71(12): 7140 - 7148. [Abstract] [Full Text] [PDF]

				N. Moghaddami, M. Costabile, P. K. Grover, H. P. A. Jersmann, Z. H. Huang, C. S. T. Hii, and A. Ferrante Unique Effect of Arachidonic Acid on Human Neutrophil TNF Receptor Expression: Up-Regulation Involving Protein Kinase C, Extracellular Signal-Regulated Kinase, and Phospholipase A2 J. Immunol., September 1, 2003; 171(5): 2616 - 2624. [Abstract] [Full Text] [PDF]

				D. T. Graves, C.-P. Chen, C. Douville, and Y. Jiang Interleukin-1 Receptor Signaling Rather than That of Tumor Necrosis Factor Is Critical in Protecting the Host from the Severe Consequences of a Polymicrobe Anaerobic Infection Infect. Immun., August 1, 2000; 68(8): 4746 - 4751. [Abstract] [Full Text] [PDF]

				J. P. Mizgerd, J. J. Peschon, and C. M. Doerschuk Roles of Tumor Necrosis Factor Receptor Signaling during Murine Escherichia coli Pneumonia Am. J. Respir. Cell Mol. Biol., January 1, 2000; 22(1): 85 - 91. [Abstract] [Full Text]

				C.-P. Chen, M. Hertzberg, Y. Jiang, and D. T. Graves Interleukin-1 and Tumor Necrosis Factor Receptor Signaling Is Not Required for Bacteria-Induced Osteoclastogenesis and Bone Loss but Is Essential for Protecting the Host from a Mixed Anaerobic Infection Am. J. Pathol., December 1, 1999; 155(6): 2145 - 2152. [Abstract] [Full Text] [PDF]

				H. P. A. Jersmann, D. A. Rathjen, and A. Ferrante Enhancement of Lipopolysaccharide-Induced Neutrophil Oxygen Radical Production by Tumor Necrosis Factor Alpha Infect. Immun., April 1, 1998; 66(4): 1744 - 1747. [Abstract] [Full Text] [PDF]

				S. S. Twining, D. P. Schulte, P. M. Wilson, B. L. Fish, and J. E. Moulder Vitamin A Deficiency Alters Rat Neutrophil Function J. Nutr., April 1, 1997; 127(4): 558 - 565. [Abstract] [Full Text]