| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 151, Issue 8 4189-4199, Copyright © 1993 by American Association of Immunologists
ARTICLES |
AL Erickson, M Houghton, QL Choo, AJ Weiner, R Ralston, E Muchmore and CM Walker
Chiron Corporation, Emeryville, CA 94608.
Hepatitis C virus (HCV)-specific CTL responses were evaluated in two chimpanzees (Pan troglodytes) during the acute and chronic phases of HCV infection. CD8+ T lymphocytes were isolated from liver tissue homogenates using anti-CD8 antibody-coated magnetic beads and then stimulated with anti-CD3 antibodies, IL-2, and irradiated human PBMC using limiting dilution culture conditions. HCV-specific cytotoxic activity of expanding CD8+ cell lines was assessed against autologous lymphoblastoid cell lines infected with recombinant vaccinia virus vectors encoding HCV Ag. CD8+ T cell lines specific for structural and nonstructural proteins of HCV were established from both animals. Cytolytic activity was blocked with anti-CD8 or anti-class I MHC antibodies, indicating that class I MHC molecules were involved in presentation of viral Ag to the CTL. Overlapping synthetic peptides were used to define a 12 amino acid segment of the nonstructural 3 (NS3) protein recognized by CTL lines from both chimpanzees. Studies with truncated peptides revealed that these CD8+ cell lines were directed against overlapping epitopes presented by distinct class I restriction elements of the chimpanzee MHC complex. CD8+ cell lines with identical specificities for an NS3 epitope were generated from one chronically infected animal at 16 and 28 wk postinfection. These results indicate that virus-specific CTL populations persist in the liver for months, but are unable to resolve chronic HCV infection.
This article has been cited by other articles:
|
|
 |
|
  A. L. Cox, T. Mosbruger, Q. Mao, Z. Liu, X.-H. Wang, H.-C. Yang, J. Sidney, A. Sette, D. Pardoll, D. L. Thomas, et al. Cellular immune selection with hepatitis C virus persistence in humans J. Exp. Med., June 6, 2005; 201(11): 1741 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Meyer-Olson, N. H. Shoukry, K. W. Brady, H. Kim, D. P. Olson, K. Hartman, A. K. Shintani, C. M. Walker, and S. A. Kalams Limited T Cell Receptor Diversity of HCV-specific T Cell Responses Is Associated with CTL Escape J. Exp. Med., August 2, 2004; 200(3): 307 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ferrari, W. Neal, J. Ottinger, A. M. Jones, B. H. Edwards, P. Goepfert, M. R. Betts, R. A. Koup, S. Buchbinder, M. J. McElrath, et al. Absence of Immunodominant Anti-Gag p17 (SL9) Responses among Gag CTL-Positive, HIV-Uninfected Vaccine Recipients Expressing the HLA-A*0201 Allele J. Immunol., August 1, 2004; 173(3): 2126 - 2133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. H. Shoukry, A. Grakoui, M. Houghton, D. Y. Chien, J. Ghrayeb, K. A. Reimann, and C. M. Walker Memory CD8+ T Cells Are Required for Protection from Persistent Hepatitis C Virus Infection J. Exp. Med., June 16, 2003; 197(12): 1645 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nascimbeni, E. Mizukoshi, M. Bosmann, M. E. Major, K. Mihalik, C. M. Rice, S. M. Feinstone, and B. Rehermann Kinetics of CD4+ and CD8+ Memory T-Cell Responses during Hepatitis C Virus Rechallenge of Previously Recovered Chimpanzees J. Virol., April 15, 2003; 77(8): 4781 - 4793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Meyer-Olson, K. W. Brady, J. T. Blackard, T. M. Allen, S. Islam, N. H. Shoukry, K. Hartman, C. M. Walker, and S. A. Kalams Analysis of the TCR {beta} Variable Gene Repertoire in Chimpanzees: Identification of Functional Homologs to Human Pseudogenes J. Immunol., April 15, 2003; 170(8): 4161 - 4169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Thimme, J. Bukh, H. C. Spangenberg, S. Wieland, J. Pemberton, C. Steiger, S. Govindarajan, R. H. Purcell, and F. V. Chisari Inaugural Article: Viral and immunological determinants of hepatitis C virus clearance, persistence, and disease PNAS, November 26, 2002; 99(24): 15661 - 15668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fournillier, C. Wychowski, D. Boucreux, T. F. Baumert, J.-C. Meunier, D. Jacobs, S. Muguet, E. Depla, and G. Inchauspe Induction of Hepatitis C Virus E1 Envelope Protein-Specific Immune Response Can Be Enhanced by Mutation of N-Glycosylation Sites J. Virol., December 15, 2001; 75(24): 12088 - 12097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Lazdina, C. Hultgren, L. Frelin, M. Chen, K. Lodin, O. Weiland, G. Leroux-Roels, J. A. Quiroga, D. L. Peterson, D. R. Milich, et al. Humoral and CD4+ T helper (Th) cell responses to the hepatitis C virus non-structural 3 (NS3) protein: NS3 primes Th1-like responses more effectively as a DNA-based immunogen than as a recombinant protein J. Gen. Virol., June 1, 2001; 82(6): 1299 - 1308. [Abstract] [Full Text]
|
|
|
|
|
|
S. B. Deitz, D. A. Dodd, S. Cooper, P. Parham, and K. Kirkegaard MHC I-dependent antigen presentation is inhibited by poliovirus protein 3A PNAS, November 22, 2000; (2000) 250483097. [Abstract] [Full Text]
|
|
|
|
|
|
X. Forns, R. Thimme, S. Govindarajan, S. U. Emerson, R. H. Purcell, F. V. Chisari, and J. Bukh Hepatitis C virus lacking the hypervariable region 1 of the second envelope protein is infectious and causes acute resolving or persistent infection in chimpanzees PNAS, November 8, 2000; (2000) 230453597. [Abstract] [Full Text]
|
|
|
|
|
|
P. Scognamiglio, D. Accapezzato, M. A. Casciaro, A. Cacciani, M. Artini, G. Bruno, M. L. Chircu, J. Sidney, S. Southwood, S. Abrignani, et al. Presence of Effector CD8+ T Cells in Hepatitis C Virus-Exposed Healthy Seronegative Donors J. Immunol., June 1, 1999; 162(11): 6681 - 6689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Major, K. Mihalik, J. Fernandez, J. Seidman, D. Kleiner, A. A. Kolykhalov, C. M. Rice, and S. M. Feinstone Long-Term Follow-Up of Chimpanzees Inoculated with the First Infectious Clone for Hepatitis C Virus J. Virol., April 1, 1999; 73(4): 3317 - 3325. [Abstract] [Full Text]
|
|
|
|
|
|
M. Selby, A. Erickson, C. Dong, S. Cooper, P. Parham, M. Houghton, and C. M. Walker Hepatitis C Virus Envelope Glycoprotein E1 Originates in the Endoplasmic Reticulum and Requires Cytoplasmic Processing for Presentation by Class I MHC Molecules J. Immunol., January 15, 1999; 162(2): 669 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Forns, R. Thimme, S. Govindarajan, S. U. Emerson, R. H. Purcell, F. V. Chisari, and J. Bukh Hepatitis C virus lacking the hypervariable region 1 of the second envelope protein is infectious and causes acute resolving or persistent infection in chimpanzees PNAS, November 21, 2000; 97(24): 13318 - 13323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Deitz, D. A. Dodd, S. Cooper, P. Parham, and K. Kirkegaard MHC I-dependent antigen presentation is inhibited by poliovirus protein 3A PNAS, December 5, 2000; 97(25): 13790 - 13795. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
