The Journal of Immunology, Vol 151, Issue 8 4055-4066, Copyright © 1993 by American Association of Immunologists

ARTICLES

Cytolytic activity of murine IL-2-producing CD4+ and CD8+ T cell clones cycles in response to IL-2

MD McKisic, DW Lancki, DC Cronin 2d and FW Fitch
Department of Pathology, University of Chicago, IL 60637.

Alloreactive or OVA-reactive cloned murine CD4+ or CD8+ T cells that produce IL-2 exhibit greatly reduced cytolytic activity after being cultured with high concentrations of rIL-2. Furthermore, such cells fail to produce lymphokines or proliferate when stimulated with Ag. The duration of this unresponsiveness to Ag correlates with the concentration of rIL-2 to which the cells were exposed; higher concentrations of rIL-2 prolong the period of unresponsiveness. The presence of ionomycin during the cytolytic assay restores lytic activity to cells rendered unresponsive by exposure to rIL-2. These results suggest that rIL-2-induced unresponsiveness to Ag is a consequence of impairment of a calcium-dependent signal important for cytolysis, proliferation, and lymphokine production. Thus, IL-2 appears to be an important lymphokine that regulates T cell responses downward as well as upward.

This article has been cited by other articles:

				R. J. Finch, P. E. Fields, and P. D. Greenberg A Transcriptional Block in the IL-2 Promoter at the -150 AP-1 Site in Effector CD8+ T Cells J. Immunol., June 1, 2001; 166(11): 6530 - 6536. [Abstract] [Full Text] [PDF]

				S. Sad and L. Krishnan Cytokine Deprivation of Naive CD8+ T Cells Promotes Minimal Cell Cycling but Maximal Cytokine Synthesis and Autonomous Proliferation Subsequently: A Mechanism of Self-Regulation J. Immunol., September 1, 1999; 163(5): 2443 - 2451. [Abstract] [Full Text] [PDF]