The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakashima, I.
Right arrow Articles by Yoshida, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakashima, I.
Right arrow Articles by Yoshida, T.

The Journal of Immunology, Vol 151, Issue 7 3511-3520, Copyright © 1993 by American Association of Immunologists

ARTICLES

Pathway of signal delivery to murine thymocytes triggered by co- crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition

I Nakashima, MY Pu, M Hamaguchi, T Iwamoto, SM Rahman, YH Zhang, M Kato, K Ohkusu, Y Katano and T Yoshida
Department of Immunology, Nagoya University School of Medicine, Japan.

The signal delivery pathway triggered by crosslinking CD3 and Thy-1 together (CD3/Thy-1 crosslinkage) on murine thymocytes for cellular DNA fragmentation/growth inhibition was analyzed. The treatment of thymocytes with herbimycin A as a specific tyrosine kinase inhibitor under suboptimum conditions before the CD3/Thy-1 crosslinkage partially but preferentially inhibited the otherwise promoted tyrosine phosphorylation of p40 and p56. Evidence was then provided that acceleration of the kinase activity of p56lck was involved in the CD3/Thy-1 crosslinkage-triggered signal. Partial characterization of p40 distinguished it from the p43 and p41 MAP kinases, the tyrosine phosphorylation of which was only marginally accelerated. Promotion of DNA fragmentation by the CD3/Thy-1 crosslinkage-triggered signal was actually ablated by the treatment with herbimycin, suggesting the obligatory involvement of the herbimycin highly sensitive kinase activity in the signal pathway. The signal induced by co-crosslinkage of CD3 and Thy-1 was also shown to be negatively biased against mature T lymphocytes, suppressing their CD3-mediated growth response. The negative signal was then found to partially attack the process of c-fos transcription as an earlier nuclear event. Interestingly, this c-fos suppression was prevented by the treatment of thymocytes with herbimycin before stimulation, for accelerated expression of c-fos. It is suggested from these results that the CD3/Thy-1 crosslinkage delivers protein tyrosine kinase-dependent negative signaling for inhibition of early and late nuclear events of both immature thymocytes and mature T lymphocytes.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. Hossain, A. A. Akhand, M. Kato, J. Du, K. Takeda, J. Wu, K. Takeuchi, W. Liu, H. Suzuki, and I. Nakashima
Arsenite Induces Apoptosis of Murine T Lymphocytes Through Membrane Raft-Linked Signaling for Activation of c-Jun Amino-Terminal Kinase
J. Immunol., October 15, 2000; 165(8): 4290 - 4297.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.