The Journal of Immunology, Vol 151, Issue 6 3206-3214, Copyright © 1993 by American Association of Immunologists

ARTICLES

Reversible expression of mouse mast cell protease 2 mRNA and protein in cultured mast cells exposed to IL-10

N Ghildyal, DS Friend, CF Nicodemus, KF Austen and RL Stevens
Department of Medicine, Harvard Medical School, Boston, MA 02115.

BALB/cJ mouse mast cells derived by culturing bone marrow progenitor cells in WEHI-3 cell-conditioned medium (BMMCW) do not contain mouse mast cell protease 2 (mMCP-2) mRNA, but these cells can be induced to express this transcript after exposure to rIL-10. To study the translation and granule accumulation of mMCP-2 in rIL-10-treated BMMC (BMMCW+IL-10), a rabbit antibody was developed to a synthetic peptide that corresponds to the novel amino acid sequence in mMCP-2 at residues 56 to 71. After affinity purification, this antibody, anti-mMCP-2(56- 71) IgG, reacted in SDS-PAGE/immunoblots against a 28-kDa protein in BMMCW+IL-10 that had the N-terminal amino acid sequence of mMCP-2. As assessed immunohistochemically, mMCP-2 protein accumulated in the secretory granules of Kirsten sarcoma virus-immortalized mouse mast cells, BMMCW+IL-10, and the mucosal mast cells present in the jejunum of Trichinella spiralis-infected BALB/cJ mice. Time course analyses of the induction of mMCP-2 mRNA and protein in BMMCW+IL-10 revealed that these cells contain a high steady-state level of mMCP-2 mRNA 24 h after their exposure to rIL-10. Although a small amount of immunodetectable mMCP-2 protein is present in the cells treated for 24 h, large amounts of this protease are not obtained until 7 days of treatment of the cells with rIL-10. Time course analyses of the loss of mMCP-2 mRNA and protein in BMMCW+IL-10 revealed that the steady-state level of mMCP-2 mRNA decreased dramatically 24 h after rIL-10 was removed from the culture medium, but that the level of mMCP-2 protein did not decline measurably until day 5 of culture. The fact that the steady-state levels of mMCP-2 mRNA and protein in BMMC can both be reversibly altered by culturing these mast cells in the presence and absence of rIL-10 suggests that the phenotype of mast cells is not fixed. Rather, it is in a dynamic state regulated by the cytokine network to which mast cells are exposed in their different microenvironments.

This article has been cited by other articles:

				B Stoffels, J Schmidt, A Nakao, A Nazir, R S Chanthaphavong, and A J Bauer Role of interleukin 10 in murine postoperative ileus Gut, May 1, 2009; 58(5): 648 - 660. [Abstract] [Full Text] [PDF]

				C.-C. Chen, M. A. Grimbaldeston, M. Tsai, I. L. Weissman, and S. J. Galli From The Cover: Identification of mast cell progenitors in adult mice PNAS, August 9, 2005; 102(32): 11408 - 11413. [Abstract] [Full Text] [PDF]

				G. W. Wong, S. Yasuda, N. Morokawa, L. Li, and R. L. Stevens Mouse Chromosome 17A3.3 Contains 13 Genes That Encode Functional Tryptic-like Serine Proteases with Distinct Tissue and Cell Expression Patterns J. Biol. Chem., January 23, 2004; 279(4): 2438 - 2452. [Abstract] [Full Text] [PDF]

				J. C. Qi, L. Li, Y. Li, K. Moore, M. C. Madigan, G. Katsoulotos, and S. A. Krilis An Antibody Raised Against In Vitro-derived Human Mast Cells Identifies Mature Mast Cells and a Population of Cells that are Fc{varepsilon}RI+, Tryptase-, and Chymase- in a Variety of Human Tissues J. Histochem. Cytochem., May 1, 2003; 51(5): 643 - 653. [Abstract] [Full Text] [PDF]

				Y. Li, L. Li, R. Wadley, S. W. Reddel, J. C. Qi, C. Archis, A. Collins, E. Clark, M. Cooley, S. Kouts, et al. Mast cells/basophils in the peripheral blood of allergic individuals who are HIV-1 susceptible due to their surface expression of CD4 and the chemokine receptors CCR3, CCR5, and CXCR4 Blood, June 1, 2001; 97(11): 3484 - 3490. [Abstract] [Full Text] [PDF]

				D. S. Friend, M. F. Gurish, K. F. Austen, J. Hunt, and R. L. Stevens Senescent Jejunal Mast Cells and Eosinophils in the Mouse Preferentially Translocate to the Spleen and Draining Lymph Node, Respectively, During the Recovery Phase of Helminth Infection J. Immunol., July 1, 2000; 165(1): 344 - 352. [Abstract] [Full Text] [PDF]

				J.-S. Kim, H. Kubota, S.-Y. Nam, K. Doi, and J. Saegusa Expression of Cytokines and Proteases in Mast Cells in the Lesion of Subcapsular Cell Hyperplasia in Mouse Adrenal Glands Toxicol Pathol, March 1, 2000; 28(2): 297 - 303. [Abstract] [PDF]

				D. M. MacIvor, S. D. Shapiro, C. T.N. Pham, A. Belaaouaj, S. N. Abraham, and T. J. Ley Normal Neutrophil Function in Cathepsin G-Deficient Mice Blood, December 15, 1999; 94(12): 4282 - 4293. [Abstract] [Full Text] [PDF]

				H. Ochi, W. M. Hirani, Q. Yuan, D. S. Friend, K. F. Austen, and J. A. Boyce T Helper Cell Type 2 Cytokine-Mediated Comitogenic Responses and Ccr3 Expression during Differentiation of Human Mast Cells in Vitro J. Exp. Med., July 19, 1999; 190(2): 267 - 280. [Abstract] [Full Text] [PDF]

				L. Li, Y. Li, S. W. Reddel, M. Cherrian, D. S. Friend, R. L. Stevens, and S. A. Krilis Identification of Basophilic Cells that Express Mast Cell Granule Proteases in the Peripheral Blood of Asthma, Allergy, and Drug-Reactive Patients J. Immunol., November 1, 1998; 161(9): 5079 - 5086. [Abstract] [Full Text] [PDF]

				D. S. Friend, N. Ghildyal, M. F. Gurish, J. Hunt, X. Hu, K. F. Austen, and R. L. Stevens Reversible Expression of Tryptases and Chymases in the Jejunal Mast Cells of Mice Infected with Trichinella spiralis J. Immunol., June 1, 1998; 160(11): 5537 - 5545. [Abstract] [Full Text] [PDF]

				J. E. Hunt, D. S. Friend, M. F. Gurish, E. Feyfant, C. Huang, N. Ghildyal, S. Stechschulte, K. F. Austen, and R. L. Stevens Mouse Mast Cell Protease 9, a Novel Member of the Chromosome 14 Family of Serine Proteases that is Selectively Expressed in Uterine Mast Cells J. Biol. Chem., November 14, 1997; 272(46): 29158 - 29166. [Abstract] [Full Text] [PDF]

				M. F. Gurish, D. S. Friend, M. Webster, N. Ghildyal, C. F. Nicodemus, and R. L. Stevens Mouse Mast Cells That Possess Segmented/Multi-lobular Nuclei Blood, July 1, 1997; 90(1): 382 - 390. [Abstract] [Full Text] [PDF]

				H. Ohtsu, A. Kuramasu, S. Suzuki, K. Igarashi, Y. Ohuchi, M. Sato, S. Tanaka, S. Nakagawa, K. Shirato, M. Yamamoto, et al. Histidine Decarboxylase Expression in Mouse Mast Cell Line P815 Is Induced by Mouse Peritoneal Cavity Incubation J. Biol. Chem., November 8, 1996; 271(45): 28439 - 28444. [Abstract] [Full Text] [PDF]

				Z. Xia, N. Ghildyal, K. F. Austen, and R. L. Stevens Post-transcriptional Regulation of Chymase Expression in Mast Cells J. Biol. Chem., April 12, 1996; 271(15): 8747 - 8753. [Abstract] [Full Text] [PDF]

				M. Murakami, R. Matsumoto, Y. Urade, K. F. Austen, and J. P. Arm c-Kit Ligand Mediates Increased Expression of Cytosolic Phospholipase A(2), Prostaglandin Endoperoxide Synthase-1, and Hematopoietic Prostaglandin D(2) Synthase and Increased IgE-dependent Prostaglandin D(2) Generation in Immature Mouse Mast Cells J. Biol. Chem., February 17, 1995; 270(7): 3239 - 3246. [Abstract] [Full Text] [PDF]