The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Christman, B. W.
Right arrow Articles by Prakash, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christman, B. W.
Right arrow Articles by Prakash, C.

The Journal of Immunology, Vol 151, Issue 4 2096-2104, Copyright © 1993 by American Association of Immunologists

ARTICLES

Prostaglandin E2 limits arachidonic acid availability and inhibits leukotriene B4 synthesis in rat alveolar macrophages by a nonphospholipase A2 mechanism

BW Christman, JW Christman, R Dworski, IA Blair and C Prakash
Department of Medicine (Division of Pulmonary and Critical Care Medicine), Vanderbilt University, Nashville, TN 37232.

Prostaglandin E2 (PGE2), a potent mediator of inflammation released in large amounts by endotoxin-stimulated alveolar macrophages (AM), has been shown to inhibit leukotriene B4 (LTB4) release by activated neutrophils. We investigated the hypothesis that LTB4 synthesis by AM can be regulated by PGE2 and performed experiments to determine the biochemical site of regulation. AM obtained from Sprague-Dawley rats were preincubated with PGE2 before stimulation with the calcium ionophore A23187. LTB4, platelet-activating factor (PAF), lysoPAF, (AA), and 5-hydroxyeicosatetraenoic acid were isolated from AM cells and supernatants, then quantified by gas chromatography/electron capture negative ion mass spectrometry. Stimulated AM released 30.50 +/- 4.52 ng LTB4/10(6) cells and were inhibited by PGE2 in a dose dependent manner. PGE2 (1 microM) inhibited LTB4 synthesis by 37% and decreased release of both 5-hydroxyeicosatetraenoic acid and arachidonic acid by stimulated AM, but did not alter synthesis of PAF or lysoPAF. These mass measurements suggest that PGE2 does not affect the activity of phospholipase A2, PAF acetyltransferase, leukotriene A4 hydrolase. We conclude that PGE2 attenuates LTB4 production in alveolar macrophages by altering the activity of lipases other than phospholipase A2. PGE2- mediated inhibition of LTB4 synthesis by AM may regulate the initiation of lung inflammation.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
C. Canetti, C. H. Serezani, R. G. Atrasz, E. S. White, D. M. Aronoff, and M. Peters-Golden
Activation of Phosphatase and Tensin Homolog on Chromosome 10 Mediates the Inhibition of Fc{gamma}R Phagocytosis by Prostaglandin E2 in Alveolar Macrophages
J. Immunol., December 15, 2007; 179(12): 8350 - 8356.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
K. Hattermann, S. Picard, M. Borgeat, P. Leclerc, M. Pouliot, and P. Borgeat
The Toll-like receptor 7/8-ligand resiquimod (R-848) primes human neutrophils for leukotriene B4, prostaglandin E2 and platelet-activating factor biosynthesis
FASEB J, May 1, 2007; 21(7): 1575 - 1585.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. N. Ballinger, D. M. Aronoff, T. R. McMillan, K. R. Cooke, K. Olkiewicz, G. B. Toews, M. Peters-Golden, and B. B. Moore
Critical Role of Prostaglandin E2 Overproduction in Impaired Pulmonary Host Response following Bone Marrow Transplantation
J. Immunol., October 15, 2006; 177(8): 5499 - 5508.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. M. Aronoff, C. Canetti, C. H. Serezani, M. Luo, and M. Peters-Golden
Cutting Edge: Macrophage Inhibition by Cyclic AMP (cAMP): Differential Roles of Protein Kinase A and Exchange Protein Directly Activated by cAMP-1
J. Immunol., January 15, 2005; 174(2): 595 - 599.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. M. Aronoff, C. Canetti, and M. Peters-Golden
Prostaglandin E2 Inhibits Alveolar Macrophage Phagocytosis through an E-Prostanoid 2 Receptor-Mediated Increase in Intracellular Cyclic AMP
J. Immunol., July 1, 2004; 173(1): 559 - 565.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Harizi, M. Juzan, J.-F. Moreau, and N. Gualde
Prostaglandins Inhibit 5-Lipoxygenase-Activating Protein Expression and Leukotriene B4 Production from Dendritic Cells Via an IL-10-Dependent Mechanism
J. Immunol., January 1, 2003; 170(1): 139 - 146.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. J. Coffey, S. M. Phare, and M. Peters-Golden
Prolonged Exposure to Lipopolysaccharide Inhibits Macrophage 5-Lipoxygenase Metabolism Via Induction of Nitric Oxide Synthesis
J. Immunol., October 1, 2000; 165(7): 3592 - 3598.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Szarek, B. Spurlock, C. A. Gruetter, and S. Lemke
Substance P and capsaicin release prostaglandin E2 from rat intrapulmonary bronchi
Am J Physiol Lung Cell Mol Physiol, November 1, 1998; 275(5): L1006 - L1012.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
G. R. Bernard, A. P. Wheeler, J. A. Russell, R. Schein, W. R. Summer, K. P. Steinberg, W. J. Fulkerson, P. E. Wright, B. W. Christman, W. D. Dupont, et al.
The Effects of Ibuprofen on the Physiology and Survival of Patients with Sepsis
N. Engl. J. Med., March 27, 1997; 336(13): 912 - 918.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.