The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     
 


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manjunath, N.
Right arrow Articles by Ardman, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manjunath, N.
Right arrow Articles by Ardman, B.

The Journal of Immunology, Vol 151, Issue 3 1528-1534, Copyright © 1993 by American Association of Immunologists


ARTICLES

Targeted disruption of CD43 gene enhances T lymphocyte adhesion

N Manjunath, RS Johnson, DE Staunton, R Pasqualini and B Ardman
Department of Medicine, New England Medical Center Hospitals, Boston, Massachusetts 02111.

CD43 is a major leukocyte surface glycoprotein thought to have important functions for T lymphocyte adhesion and activation. We investigated the function of CD43 by using gene targeting to eliminate CD43 expression in the human T lymphocyte line CEM and then testing their adhesive phenotype. Loss of CD43 expression by the CEM cells enhanced their homotypic adhesion and binding to two distinct ligands, fibronectin and HIV-1 gp120. The enhanced homotypic adhesion was blocked specifically by an anti-beta 1 integrin mAb, and the enhanced binding to fibronectin and gp120 was blocked specifically by anti-beta 1 integrin and anti-CD4 mAb, respectively. Partial reconstitution of CD43 expression in the CD43-negative cells resulted in a corresponding reversion to a less adhesive phenotype. These data suggest that CD43 interferes with T lymphocyte adhesion and that CD43 can regulate lymphocyte adhesion by providing a threshold that must be overcome for cell-cell and cell-ligand interactions to occur.


This article has been cited by other articles:


Home page
BloodHome page
P. D. Mody, J. L. Cannon, H. S. Bandukwala, K. M. Blaine, A. B. Schilling, K. Swier, and A. I. Sperling
Signaling through CD43 regulates CD4 T-cell trafficking
Blood, October 15, 2007; 110(8): 2974 - 2982.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. Matsumoto, A. Shigeta, Y. Furukawa, T. Tanaka, M. Miyasaka, and T. Hirata
CD43 Collaborates with P-Selectin Glycoprotein Ligand-1 to Mediate E-Selectin-Dependent T Cell Migration into Inflamed Skin
J. Immunol., February 15, 2007; 178(4): 2499 - 2506.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
J. D. Hernandez, J. T. Nguyen, J. He, W. Wang, B. Ardman, J. M. Green, M. Fukuda, and L. G. Baum
Galectin-1 Binds Different CD43 Glycoforms to Cluster CD43 and Regulate T Cell Death
J. Immunol., October 15, 2006; 177(8): 5328 - 5336.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
R. C. Fuhlbrigge, S. L. King, R. Sackstein, and T. S. Kupper
CD43 is a ligand for E-selectin on CLA+ human T cells
Blood, February 15, 2006; 107(4): 1421 - 1426.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
B. N. Stillman, D. K. Hsu, M. Pang, C. F. Brewer, P. Johnson, F.-T. Liu, and L. G. Baum
Galectin-3 and Galectin-1 Bind Distinct Cell Surface Glycoprotein Receptors to Induce T Cell Death
J. Immunol., January 15, 2006; 176(2): 778 - 789.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. Matsumoto, K. Atarashi, E. Umemoto, Y. Furukawa, A. Shigeta, M. Miyasaka, and T. Hirata
CD43 Functions as a Ligand for E-Selectin on Activated T Cells
J. Immunol., December 15, 2005; 175(12): 8042 - 8050.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
A. K. Randhawa, H. J. Ziltener, J. S. Merzaban, and R. W. Stokes
CD43 Is Required for Optimal Growth Inhibition of Mycobacterium tuberculosis in Macrophages and in Mice
J. Immunol., August 1, 2005; 175(3): 1805 - 1812.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
J. Tong, E. J. Allenspach, S. M. Takahashi, P. D. Mody, C. Park, J. K. Burkhardt, and A. I. Sperling
CD43 Regulation of T Cell Activation Is Not through Steric Inhibition of T Cell-APC Interactions but through an Intracellular Mechanism
J. Exp. Med., May 3, 2004; 199(9): 1277 - 1283.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. E. Cruz-Munoz, E. Salas-Vidal, N. Salaiza-Suazo, I. Becker, G. Pedraza-Alva, and Y. Rosenstein
The CD43 Coreceptor Molecule Recruits the {zeta}-Chain as Part of Its Signaling Pathway
J. Immunol., August 15, 2003; 171(4): 1901 - 1908.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
N. Da Silva, A. Bharti, and C. S. Shelley
hnRNP-K and Puralpha act together to repress the transcriptional activity of the CD43 gene promoter
Blood, November 15, 2002; 100(10): 3536 - 3544.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
N. D. L. Savage, S. L. Kimzey, S. K. Bromley, K. G. Johnson, M. L. Dustin, and J. M. Green
Polar Redistribution of the Sialoglycoprotein CD43: Implications for T Cell Function
J. Immunol., April 15, 2002; 168(8): 3740 - 3746.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
C. S. Shelley, J. M. Teodoridis, H. Park, O. C. Farokhzad, E. P. Bottinger, and M. A. Arnaout
During Differentiation of the Monocytic Cell Line U937, Pur{alpha} Mediates Induction of the CD11c{beta}2 Integrin Gene Promoter
J. Immunol., April 15, 2002; 168(8): 3887 - 3893.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
D. A. Carlow, S. Y. Corbel, and H. J. Ziltener
Absence of CD43 Fails to Alter T Cell Development and Responsiveness
J. Immunol., January 1, 2001; 166(1): 256 - 261.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
M. Fukuoka, K. Fukudome, Y. Yamashita, M. Tokushima, K. Miyake, and M. Kimoto
Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines
Blood, December 15, 2000; 96(13): 4267 - 4275.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
O. C. Farokhzad, J. M. Teodoridis, H. Park, M. A. Arnaout, and C. S. Shelley
CD43 gene expression is mediated by a nuclear factor which binds pyrimidine-rich single-stranded DNA
Nucleic Acids Res., June 1, 2000; 28(11): 2256 - 2267.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. Fabbi, J. Geginat, M. Tiso, D. Ramarli, D. Parent, A. Bargellesi, and E. Remold-O'Donnell
8B4/20, A Private CD43 Epitope on Developing Human Thymocytes, Is Involved in Thymocyte Maturation
J. Immunol., December 1, 1999; 163(11): 5964 - 5970.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
G. G. Johnson, A. Mikulowska, E. C. Butcher, L. M. McEvoy, and S. A. Michie
Anti-CD43 Monoclonal Antibody L11 Blocks Migration of T Cells to Inflamed Pancreatic Islets and Prevents Development of Diabetes in Nonobese Diabetic Mice
J. Immunol., November 15, 1999; 163(10): 5678 - 5685.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
M. Nieto, J. L. Rodriguez-Fernandez, F. Navarro, D. Sancho, J. M.R. Frade, M. Mellado, C. Martinez-A, C. Cabanas, and F. Sanchez-Madrid
Signaling Through CD43 Induces Natural Killer Cell Activation, Chemokine Release, and PYK-2 Activation
Blood, October 15, 1999; 94(8): 2767 - 2777.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
S. Corinti, E. Fanales-Belasio, C. Albanesi, A. Cavani, P. Angelisova, and G. Girolomoni
Cross-Linking of Membrane CD43 Mediates Dendritic Cell Maturation
J. Immunol., June 1, 1999; 162(11): 6331 - 6336.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
N. Anzai, A. Gotoh, H. Shibayama, and H. E. Broxmeyer
Modulation of Integrin Function in Hematopoietic Progenitor Cells by CD43 Engagement: Possible Involvement of Protein Tyrosine Kinase and Phospholipase C-{gamma}
Blood, May 15, 1999; 93(10): 3317 - 3326.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
J. Walker and J. M. Green
Structural Requirements for CD43 Function
J. Immunol., April 1, 1999; 162(7): 4109 - 4114.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
S. M. Morgan, U. Samulowitz, L. Darley, D. L. Simmons, and D. Vestweber
Biochemical Characterization and Molecular Cloning of a Novel Endothelial-Specific Sialomucin
Blood, January 1, 1999; 93(1): 165 - 175.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
A. I. Sperling, J. R. Sedy, N. Manjunath, A. Kupfer, B. Ardman, and J. K. Burkhardt
Cutting Edge: TCR Signaling Induces Selective Exclusion of CD43 from the T Cell-Antigen-Presenting Cell Contact Site
J. Immunol., December 15, 1998; 161(12): 6459 - 6462.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
R. C. Woodman, B. Johnston, M. J. Hickey, D. Teoh, P. Reinhardt, B. Y. Poon, and P. Kubes
The Functional Paradox of CD43 in Leukocyte Recruitment: A Study Using CD43-deficient Mice
J. Exp. Med., December 7, 1998; 188(11): 2181 - 2186.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
G. Pedraza-Alva, L. B. Merida, S. J. Burakoff, and Y. Rosenstein
T Cell Activation through the CD43 Molecule Leads to Vav Tyrosine Phosphorylation and Mitogen-activated Protein Kinase Pathway Activation
J. Biol. Chem., June 5, 1998; 273(23): 14218 - 14224.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Komatsu, C. A. C. Carraway, N. L. Fregien, and K. L. Carraway
Reversible Disruption of Cell-Matrix and Cell-Cell Interactions by Overexpression of Sialomucin Complex
J. Biol. Chem., December 26, 1997; 272(52): 33245 - 33254.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
L. M. McEvoy, M. A. Jutila, P. S. Tsao, J. P. Cooke, and E. C. Butcher
Anti-CD43 Inhibits Monocyte-Endothelial Adhesion in Inflammation and Atherogenesis
Blood, November 1, 1997; 90(9): 3587 - 3594.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. J. Brown, W. W. Shuford, W.-C. Wang, S. G. Nadler, T. S. Bailey, H. Marquardt, and R. S. Mittler
Characterization of a CD43/Leukosialin-mediated Pathway for Inducing Apoptosis in Human T-Lymphoblastoid Cells
J. Biol. Chem., November 1, 1996; 271(44): 27686 - 27695.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. Baeckström, K. Zhang, N. Asker, U. Rüetschi, M. Ek, and G. C. Hansson
Expression of the Leukocyte-associated Sialoglycoprotein CD43 by a Colon Carcinoma Cell Line
J. Biol. Chem., June 9, 1995; 270(23): 13688 - 13692.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
J. Weitzman, A Chen, and M. Hemler
Investigation of the role of beta 1 integrins in cell-cell adhesion
J. Cell Sci., January 11, 1995; 108(11): 3635 - 3644.
[Abstract] [PDF]


Home page
J. Biol. Chem.Home page
M. A. Santana, G. Pedraza-Alva, N. Olivares-Zavaleta, V. Madrid-Marina, V. Horejsi, S. J. Burakoff, and Y. Rosenstein
CD43-mediated Signals Induce DNA Binding Activity of AP-1, NF-AT, and NFkappa B Transcription Factors in Human T Lymphocytes
J. Biol. Chem., September 29, 2000; 275(40): 31460 - 31468.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
G. Pedraza-Alva, S. Sawasdikosol, Y. C. Liu, L. B. Merida, M. E. Cruz-Munoz, F. Oceguera-Yanez, S. J. Burakoff, and Y. Rosenstein
Regulation of Cbl Molecular Interactions by the Co-receptor Molecule CD43 in Human T Cells
J. Biol. Chem., January 5, 2001; 276(1): 729 - 737.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.