| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 151, Issue 11 6175-6184, Copyright © 1993 by American Association of Immunologists
ARTICLES |
JC Reece, HM Geysen and SJ Rodda
Chiron Mimotopes Pty. Ltd., Clayton, Victoria, Australia.
Progress on the mapping of Th epitopes of tetanus toxin (tt) has been slow due to reliance on studies of clones. In this paper, human Th cell epitopes of tt were mapped using proliferation tests on PBMC in response to synthetic peptides. PBMC from nine donors were tested over the entire set of tt homologous overlapping dodecapeptides. The 1304 peptides were initially tested as 66 pools, each containing an average of 20 peptides. PBMC from individual donors responded to as few as 1 and as many as 17 of the 66 peptide pools. The sequences responsible for proliferation were identified for the two most frequently recognized pools, and for another two pools within a major immunodominant region. Three new epitope sequences were mapped in detail and based on their recognition by most individuals are likely to be promiscuous. A cocktail of peptides including the newly identified Th cell epitopes was able to induce proliferation in PBMC from 24 of 31 tetanus toxoid (TT)-responsive donors. This cocktail is a chemically defined reagent that can be used to quantitate in vitro Ag-specific Th cells in PBMC from most subjects, and may thus be useful for serial measurements of specific immunity such as in subjects undergoing immunotherapy or immunosuppressive treatment.
This article has been cited by other articles:
|
|
 |
|
  E. A. James, J. Bui, D. Berger, L. Huston, M. Roti, and W. W. Kwok Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition Int. Immunol., November 1, 2007; 19(11): 1291 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Barbey, E. Pradervand, N. Barbier, and F. Spertini Ex Vivo Monitoring of Antigen-Specific CD4+ T Cells after Recall Immunization with Tetanus Toxoid Clin. Vaccine Immunol., September 1, 2007; 14(9): 1108 - 1116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dakappagari, T. Maruyama, M. Renshaw, P. Tacken, C. Figdor, R. Torensma, M. A. Wild, D. Wu, K. Bowdish, and A. Kretz-Rommel Internalizing Antibodies to the C-Type Lectins, L-SIGN and DC-SIGN, Inhibit Viral Glycoprotein Binding and Deliver Antigen to Human Dendritic Cells for the Induction of T Cell Responses J. Immunol., January 1, 2006; 176(1): 426 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Salazar, J. Fikes, S. Southwood, G. Ishioka, K. L. Knutson, T. A. Gooley, K. Schiffman, and M. L. Disis Immunization of Cancer Patients with HER-2/neu-Derived Peptides Demonstrating High-Affinity Binding to Multiple Class II Alleles Clin. Cancer Res., November 15, 2003; 9(15): 5559 - 5565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Goetsch, J. F. Haeuw, T. Champion, C. Lacheny, T. N'Guyen, A. Beck, and N. Corvaia Identification of B- and T-Cell Epitopes of BB, a Carrier Protein Derived from the G Protein of Streptococcus Strain G148 Clin. Vaccine Immunol., January 1, 2003; 10(1): 125 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. La Rosa, Z. Wang, J. C. Brewer, S. F. Lacey, M. C. Villacres, R. Sharan, R. Krishnan, M. Crooks, S. Markel, R. Maas, et al. Preclinical development of an adjuvant-free peptide vaccine with activity against CMV pp65 in HLA transgenic mice Blood, November 15, 2002; 100(10): 3681 - 3689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Disis, T. A. Gooley, K. Rinn, D. Davis, M. Piepkorn, M. A. Cheever, K. L. Knutson, and K. Schiffman Generation of T-Cell Immunity to the HER-2/neu Protein After Active Immunization With HER-2/neu Peptide-Based Vaccines J. Clin. Oncol., June 1, 2002; 20(11): 2624 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Disis, K. Rinn, K. L. Knutson, D. Davis, D. Caron, C. dela Rosa, and K. Schiffman Flt3 ligand as a vaccine adjuvant in association with HER-2/neu peptide-based vaccines in patients with HER-2/neu-overexpressing cancers Blood, April 15, 2002; 99(8): 2845 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. McNeel, L. D. Nguyen, and M. L. Disis Identification of T Helper Epitopes from Prostatic Acid Phosphatase Cancer Res., July 1, 2001; 61(13): 5161 - 5167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Velders, S. Weijzen, G. L. Eiben, A. G. Elmishad, P.-M. Kloetzel, T. Higgins, R. B. Ciccarelli, M. Evans, S. Man, L. Smith, et al. Defined Flanking Spacers and Enhanced Proteolysis Is Essential for Eradication of Established Tumors by an Epitope String DNA Vaccine J. Immunol., May 1, 2001; 166(9): 5366 - 5373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Woodfolk, S.-S. J. Sung, D. C. Benjamin, J. K. Lee, and T. A. E. Platts-Mills Distinct Human T Cell Repertoires Mediate Immediate and Delayed-Type Hypersensitivity to the Trichophyton Antigen, Tri r 2 J. Immunol., October 15, 2000; 165(8): 4379 - 4387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mosorin, H.-M. Surcel, A. Laurila, M. Lehtinen, R. Karttunen, J. Juvonen, J. Paavonen, R. P. Morrison, P. Saikku, and T. Juvonen Detection of Chlamydia pneumoniae-Reactive T Lymphocytes in Human Atherosclerotic Plaques of Carotid Artery Arterioscler. Thromb. Vasc. Biol., April 1, 2000; 20(4): 1061 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Zaia, J.G. P. Sissons, S. Riddell, D. J. Diamond, M.R. Wills, A.J. Carmichael, M.P. Weekes, M. Gandhi, C. La Rosa, M. Villacres, et al. Status of Cytomegalovirus Prevention and Treatment in 2000 Hematology, January 1, 2000; 2000(1): 339 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Disis, K. H. Grabstein, P. R. Sleath, and M. A. Cheever Generation of Immunity to the HER-2/neu Oncogenic Protein in Patients with Breast and Ovarian Cancer Using a Peptide-based Vaccine Clin. Cancer Res., June 1, 1999; 5(6): 1289 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lo-Man, S. Bay, S. Vichier-Guerre, E. Deriaud, D. Cantacuzene, and C. Leclerc A Fully Synthetic Immunogen Carrying a Carcinoma-associated Carbohydrate for Active Specific Immunotherapy1 Cancer Res., April 1, 1999; 59(7): 1520 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Fanger, C. Liu, P. M. Guyre, K. Wardwell, J. O'Neil, T. L. Guo, T. P. Christian, S. P. Mudzinski, and E. J. Gosselin Activation of Human T Cells by Major Histocompatability Complex Class II Expressing Neutrophils: Proliferation in the Presence of Superantigen, But Not Tetanus Toxoid Blood, June 1, 1997; 89(11): 4128 - 4135. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
