The Journal of Immunology, Vol 151, Issue 10 5716-5723, Copyright © 1993 by American Association of Immunologists

ARTICLES

Activated eosinophils evoke chloride secretion in model intestinal epithelia primarily via regulated release of 5'-AMP

MB Resnick, SP Colgan, TW Patapoff, RJ Mrsny, CS Awtrey, C Delp-Archer, PF Weller and JL Madara
Department of Pathology, Brigham and Women's Hospital, Boston, MA.

Eosinophils may be prominent in intestinal diseases including allergic gastroenteritis, inflammatory bowel disease, enteritis associated with hypereosinophilic syndromes (HES), and parasitic diseases. Unlike normal blood eosinophils, those that circulate in HES and those that infiltrate inflamed tissue exhibit an "activated" phenotype. To model intestinal epithelial-eosinophil interactions, we used peripheral blood eosinophils and human crypt-like T84 epithelial cell-line monolayers. Eosinophils from normal, mildly atopic donors, only if activated by PMA or primed with granulocyte-macrophage-CSF for 48 h, as well as eosinophils from HES patients elicited a short circuit current when applied apically to T84 monolayers. This eosinophil-derived bioactivity, which was transferable in cell-free supernatants and in < 1000 m.w. ultrafiltrates, stimulated electrogenic Cl- secretion, as indicated by inhibition with basolateral bumetanide or gluconate substitution and by enhancement of the rate constant for 125I efflux from preloaded T84 cells. This secretagogue activity was blocked in both intact activated eosinophils and in eosinophil-conditioned supernatants, by 8-phenyl-theophylline, indicating involvement of an adenosine receptor. Ion exchange and reversed-phase HPLC analyses demonstrated that eosinophil supernatant ultrafiltrates contained elevated levels of 5'-AMP that was converted to adenosine after incubation with epithelium. Inhibition of epithelial apical membrane ecto-5'-nucleotidase ablated the conversion to adenosine. These studies establish that activated eosinophils elicit Cl- secretion from intestinal epithelial and that 5'-AMP released by eosinophils followed by its conversion to adenosine at the epithelial surface is the basis for this response.

This article has been cited by other articles:

				J. B. Volmer, L. F. Thompson, and M. R. Blackburn Ecto-5'-Nucleotidase (CD73)-Mediated Adenosine Production Is Tissue Protective in a Model of Bleomycin-Induced Lung Injury J. Immunol., April 1, 2006; 176(7): 4449 - 4458. [Abstract] [Full Text] [PDF]

				H. Inbe, S. Watanabe, M. Miyawaki, E. Tanabe, and J. A. Encinas Identification and Characterization of a Cell-Surface Receptor, P2Y15, for AMP and Adenosine J. Biol. Chem., May 7, 2004; 279(19): 19790 - 19799. [Abstract] [Full Text] [PDF]

				G. R. Dubyak Knock-Out Mice Reveal Tissue-Specific Roles of P2Y Receptor Subtypes in Different Epithelia Mol. Pharmacol., April 1, 2003; 63(4): 773 - 776. [Full Text] [PDF]

				P. F. Lennon, C. T. Taylor, G. L. Stahl, and S. P. Colgan Neutrophil-derived 5'-Adenosine Monophosphate Promotes Endothelial Barrier Function via CD73-mediated Conversion to Adenosine and Endothelial A2B Receptor Activation J. Exp. Med., October 19, 1998; 188(8): 1433 - 1443. [Abstract] [Full Text] [PDF]

				M. Zareie, D. M. McKay, G. G. Kovarik, and M. H. Perdue Monocyte/macrophages evoke epithelial dysfunction: indirect role of tumor necrosis factor-alpha Am J Physiol Cell Physiol, October 1, 1998; 275(4): C932 - C939. [Abstract] [Full Text] [PDF]

				D. C. Devor and R. A. Frizzell Modulation of K+ channels by arachidonic acid in T84 cells. II. Activation of a Ca2+-independent K+ channel Am J Physiol Cell Physiol, January 1, 1998; 274(1): C149 - C160. [Abstract] [Full Text] [PDF]