The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peppel, K.
Right arrow Articles by Beutler, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peppel, K.
Right arrow Articles by Beutler, B.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Compound via MeSH
*Substance via MeSH

The Journal of Immunology, Vol 151, Issue 10 5699-5703, Copyright © 1993 by American Association of Immunologists

ARTICLES

Expression of a TNF inhibitor in transgenic mice

K Peppel, A Poltorak, I Melhado, F Jirik and B Beutler
Howard Hughes Medical Institute, Dallas, TX 75235.

We previously reported that a chimeric protein consisting of the human p55 TNF receptor covalently linked to a murine IgG1 Fc heavy chain acts as an efficient TNF inhibitor, as a result of its high binding affinity for native TNF trimers of both murine and human origin. A transgenic mouse line constitutively expressing the inhibitor from a cytomegalovirus promoter was established. All organs examined expressed the transgene. TNF inhibitory activity was easily detected in plasma of transgenic animals but not in plasma of nontransgenic littermates. This founder line was not found to have any obvious phenotype. Specifically, transgenic mice born to wild-type or transgenic females were indistinguishable from nontransgenic littermates with respect to their size at birth, at three months and at six months of age, with respect to the size and morphology of their lymphoid organs and with respect to their hematocrit, white blood cell count, and differential. Although TNF is known to be constitutively expressed by cells of the thymus and trophoblast, the lack of a clear phenotype in association with the constitutive expression of a TNF inhibitor suggests that TNF may be dispensable in early development.


This article has been cited by other articles:


Home page
 IOVSHome page
S. A. Rayner, D. F. P. Larkin, and A. J. T. George
TNF Receptor Secretion after Ex Vivo Adenoviral Gene Transfer to Cornea and Effect on In Vivo Graft Survival
Invest. Ophthalmol. Vis. Sci., June 1, 2001; 42(7): 1568 - 1573.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Ettinger, J. L. Browning, S. A. Michie, W. van Ewijk, and H. O. McDevitt
Disrupted splenic architecture, but normal lymph node development in mice expressing a soluble lymphotoxin-beta receptor-IgG1 fusion protein
PNAS, November 12, 1996; 93(23): 13102 - 13107.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
B Beutler and C van Huffel
Unraveling function in the TNF ligand and receptor families
Science, April 29, 1994; 264(5159): 667 - 668.
[PDF]

Home page
 ScienceHome page
P De Togni, J Goellner, N. Ruddle, P. Streeter, A Fick, S Mariathasan, S. Smith, R Carlson, L. Shornick, J Strauss-Schoenberger, et al.
Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin
Science, April 29, 1994; 264(5159): 703 - 707.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.