The Journal of Immunology, Vol 151, Issue 10 5172-5185, Copyright © 1993 by American Association of Immunologists

ARTICLES

Differential induction of VCAM-1 on human iliac venous and arterial endothelial cells and its role in adhesion

IA Hauser, DR Johnson and JA Madri
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510.

Venous and arterial large vessel endothelial cells (EC) were compared for their constitutive and TNF-alpha-induced expression of the cell- surface adhesion molecules ICAM-1 and -2, VCAM-1 and ELAM-1 by FACS analysis. Human iliac venous and arterial EC (HIVEC and HIAEC) constitutively express ICAM-1 and ICAM-2. TNF-alpha increases the expression of ICAM-1, but not ICAM-2, and induces the expression of ELAM-1 on both EC types. However, TNF-alpha induces VCAM-1 cell-surface expression and mRNA only in venous, but not in arterial EC. We next investigated the function of these adhesion molecules and their ligands, LFA-1, very late activation Ag (WLA-L) and sialylated Lewis x glycoprotein (sLe(x)), in adhesion assays with the monocyte-like cell line U937. Untreated U937 cells do not adhere to untreated HIVEC or HIAEC and adhesion is much lower to TNF-alpha-treated arterial than to TNF-alpha-treated venous EC. In adhesion-inhibition assays we demonstrate that U937 cell adhesion to TNF-alpha-treated HIVEC is mediated by VCAM-1/VLA-4 and ELAM-1/sLe(x) interaction, whereas the lower adhesion to TNF-alpha-treated HIAEC is only mediated by ELAM- 1/sLe(x) interaction. U937 cells treated with the phorbol ester PMA for 3 days adhere to both HIVEC and HIAEC; this adhesion is mediated by LFA- 1 interaction with ICAM-1 and/or -2. Adhesion of PMA-treated U937 cells is increased by TNF-alpha treatment of EC. This increased adhesion is mediated in part by the TNF-alpha-induced VCAM-1 expression on venous EC. Therefore, the cell-surface adhesion molecule VCAM-1 is differentially induced on these two EC types and the differential expression is functionally important in U937 cell adhesion.

This article has been cited by other articles:

				R. J.R. Singh, J. C. Mason, E. A. Lidington, D. R. Edwards, R. K. Nuttall, R. Khokha, V. Knauper, G. Murphy, and J. Gavrilovic Cytokine stimulated vascular cell adhesion molecule-1 (VCAM-1) ectodomain release is regulated by TIMP-3 Cardiovasc Res, July 1, 2005; 67(1): 39 - 49. [Abstract] [Full Text] [PDF]

				P. Rafiee, H. Ogawa, J. Heidemann, M. S. Li, M. Aslam, T. H. Lamirand, P. J. Fisher, S. J. Graewin, M. B. Dwinell, C. P. Johnson, et al. Isolation and characterization of human esophageal microvascular endothelial cells: mechanisms of inflammatory activation Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1277 - G1292. [Abstract] [Full Text] [PDF]

				C. K. D. Ng, S. S. Deshpande, K. Irani, and B. R. Alevriadou Adhesion of flowing monocytes to hypoxia-reoxygenation-exposed endothelial cells: role of Rac1, ROS, and VCAM-1 Am J Physiol Cell Physiol, July 1, 2002; 283(1): C93 - C102. [Abstract] [Full Text] [PDF]

				K. J. Purdie, G. St.J. Whitley, A. P. Johnstone, and J. E. Cartwright Hepatocyte growth factor-induced endothelial cell motility is mediated by the upregulation of inducible nitric oxide synthase expression Cardiovasc Res, June 1, 2002; 54(3): 659 - 668. [Abstract] [Full Text] [PDF]

				J. A. Ronald, C. V. Ionescu, K. A. Rogers, and M. Sandig Differential regulation of transendothelial migration of THP-1 cells by ICAM-1/LFA-1 and VCAM-1/VLA-4 J. Leukoc. Biol., October 1, 2001; 70(4): 601 - 609. [Abstract] [Full Text] [PDF]

				J. J. Moreno Antiflammin-2 Prevents HL-60 Adhesion to Endothelial Cells and Prostanoid Production Induced by Lipopolysaccharides J. Pharmacol. Exp. Ther., March 1, 2001; 296(3): 884 - 889. [Abstract] [Full Text]

				I. Hauser, L. Renders, H. Radeke, R. Sterzel, and M. Goppelt-Struebe Mycophenolate mofetil inhibits rat and human mesangial cell proliferation by guanosine depletion Nephrol. Dial. Transplant., January 1, 1999; 14(1): 58 - 63. [Abstract] [PDF]

				S. Lechleitner, J. Gille, D. R. Johnson, and P. Petzelbauer Interferon Enhances Tumor Necrosis Factor-induced Vascular Cell Adhesion Molecule 1 (CD106) Expression in Human Endothelial Cells by an Interferon-related Factor 1-dependent Pathway J. Exp. Med., June 15, 1998; 187(12): 2023 - 2030. [Abstract] [Full Text] [PDF]

				T. Arai, A. K. Bhunia, S. Chatterjee, and G. B. Bulkley Lactosylceramide Stimulates Human Neutrophils to Upregulate Mac-1, Adhere to Endothelium, and Generate Reactive Oxygen Metabolites In Vitro Circ. Res., March 23, 1998; 82(5): 540 - 547. [Abstract] [Full Text] [PDF]

				D. M. Smalley, J. H.-C. Lin, M. L. Curtis, Y. Kobari, M. B. Stemerman, and K. A. Pritchard Jr Native LDL Increases Endothelial Cell Adhesiveness by Inducing Intercellular Adhesion Molecule–1 Arterioscler. Thromb. Vasc. Biol., April 1, 1996; 16(4): 585 - 590. [Abstract] [Full Text]