The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoffman, O. A.
Right arrow Articles by Limper, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoffman, O. A.
Right arrow Articles by Limper, A. H.

The Journal of Immunology, Vol 150, Issue 9 3932-3940, Copyright © 1993 by American Association of Immunologists

ARTICLES

Pneumocystis carinii stimulates tumor necrosis factor-alpha release from alveolar macrophages through a beta-glucan-mediated mechanism

OA Hoffman, JE Standing and AH Limper
Division of Thoracic Diseases and Internal Medicine, Mayo Clinic and Foundation, Rochester, MN 55905.

Recent studies suggest that TNF-alpha plays a central role in host defenses during Pneumocystis carinii pneumonia. To determine whether P. carinii directly stimulates TNF-alpha secretion, rat alveolar macrophages were cultured in the presence of purified P. carinii. Whereas unstimulated alveolar macrophages released only 13.0 +/- 2.7 pg/ml of TNF-alpha into the medium, macrophages stimulated with P. carinii released 108.2 +/- 20.4 pg/ml of TNF-alpha after overnight culture (p = 0.0001). Maximal TNF-alpha release was observed after 8 h of incubation and required a P. carinii: macrophage ratio of at least 2.5:1. Autoclaved P. carinii were also able to trigger TNF-alpha release from macrophages albeit at a reduced level. In view of recent evidence that P. carinii is phylogenetically related to the fungi and contains a beta-glucan-rich cell wall, we hypothesized that TNF-alpha release might in part be mediated by this cell wall component. Preincubation of macrophages with particulate beta-glucan derived from Baker's yeast resulted in complete inhibition of TNF-alpha release in response to P. carinii. In addition, digestion of P. carinii with zymolyase, a preparation containing predominantly beta-glucanase activity, substantially reduced the ability of P. carinii to cause TNF- alpha release from macrophages. In a similar manner, macrophages incubated with P. carinii in the presence of laminariheptaose, an oligosaccharide that binds to macrophage beta-glucan receptors, also displayed decreased TNF-alpha release. Interestingly, TNF-alpha release may not be completely linked to the adherence of the organism to macrophages. Particulate beta-glucan significantly reduced P. carinii adherence to macrophages and also impaired TNF-alpha release. However, yeast mannan also significantly reduced P. carinii adherence to macrophages and also impaired TNF-alpha release. However, yeast mannan also significantly reduced P. carinii adherence but had no effect on TNF-alpha release. These data demonstrate that P. carinii can directly stimulate the secretion of TNF-alpha from alveolar macrophages and that this effect is largely mediated by beta-glucan components of the P. carinii cell wall.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
G. S. Pryhuber, H. L. Huyck, S. Bhagwat, M. A. O'Reilly, J. N. Finkelstein, F. Gigliotti, and T. W. Wright
Parenchymal Cell TNF Receptors Contribute to Inflammatory Cell Recruitment and Respiratory Failure in Pneumocystis carinii-Induced Pneumonia
J. Immunol., July 15, 2008; 181(2): 1409 - 1419.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Hollifield, E. B. Ghanem, W. J. S. de Villiers, and B. A. Garvy
Scavenger Receptor A Dampens Induction of Inflammation in Response to the Fungal Pathogen Pneumocystis carinii
Infect. Immun., August 1, 2007; 75(8): 3999 - 4005.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. M. Empey, M. Hollifield, and B. A. Garvy
Exogenous Heat-Killed Escherichia coli Improves Alveolar Macrophage Activity and Reduces Pneumocystis carinii Lung Burden in Infant Mice
Infect. Immun., July 1, 2007; 75(7): 3382 - 3393.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
S. Tasaka, N. Hasegawa, S. Kobayashi, W. Yamada, T. Nishimura, T. Takeuchi, and A. Ishizaka
Serum Indicators for the Diagnosis of Pneumocystis Pneumonia
Chest, April 1, 2007; 131(4): 1173 - 1180.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. R. Rapaka, E. S. Goetzman, M. Zheng, J. Vockley, L. McKinley, J. K. Kolls, and C. Steele
Enhanced Defense against Pneumocystis carinii Mediated by a Novel Dectin-1 Receptor Fc Fusion Protein
J. Immunol., March 15, 2007; 178(6): 3702 - 3712.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. M. Carmona, R. Vassallo, Z. Vuk-Pavlovic, J. E. Standing, T. J. Kottom, and A. H. Limper
Pneumocystis Cell Wall beta-Glucans Induce Dendritic Cell Costimulatory Molecule Expression and Inflammatory Activation through a Fas-Fas Ligand Mechanism
J. Immunol., July 1, 2006; 177(1): 459 - 467.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. E. Lasbury, P. J. Durant, C. A. Ray, D. Tschang, R. Schwendener, and C.-H. Lee
Suppression of Alveolar Macrophage Apoptosis Prolongs Survival of Rats and Mice with Pneumocystis Pneumonia.
J. Immunol., June 1, 2006; 176(11): 6443 - 6453.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Zhang, S.-H. Wang, M. E. Lasbury, D. Tschang, C.-P. Liao, P. J. Durant, and C.-H. Lee
Toll-Like Receptor 2 Mediates Alveolar Macrophage Response to Pneumocystis murina
Infect. Immun., March 1, 2006; 74(3): 1857 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. Zhang, S. D. Tachado, N. Patel, J. Zhu, A. Imrich, P. Manfruelli, M. Cushion, T. B. Kinane, and H. Koziel
Negative regulatory role of mannose receptors on human alveolar macrophage proinflammatory cytokine release in vitro
J. Leukoc. Biol., September 1, 2005; 78(3): 665 - 674.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
C. F. Thomas Jr. and A. H. Limper
Pneumocystis Pneumonia
N. Engl. J. Med., June 10, 2004; 350(24): 2487 - 2498.
[Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Zhang, J. Zhu, A. Imrich, M. Cushion, T. B. Kinane, and H. Koziel
Pneumocystis Activates Human Alveolar Macrophage NF-{kappa}B Signaling through Mannose Receptors
Infect. Immun., June 1, 2004; 72(6): 3147 - 3160.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. E. Lasbury, P. Lin, D. Tschang, P. J. Durant, and C.-H. Lee
Effect of Bronchoalveolar Lavage Fluid from Pneumocystis carinii- Infected Hosts on Phagocytic Activity of Alveolar Macrophages
Infect. Immun., April 1, 2004; 72(4): 2140 - 2147.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. W. Wright, G. S. Pryhuber, P. R. Chess, Z. Wang, R. H. Notter, and F. Gigliotti
TNF Receptor Signaling Contributes to Chemokine Secretion, Inflammation, and Respiratory Deficits during Pneumocystis Pneumonia
J. Immunol., February 15, 2004; 172(4): 2511 - 2521.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. Steele, L. Marrero, S. Swain, A. G. Harmsen, M. Zheng, G. D. Brown, S. Gordon, J. E. Shellito, and J. K. Kolls
Alveolar Macrophage-mediated Killing of Pneumocystis carinii f. sp. muris Involves Molecular Recognition by the Dectin-1 {beta}-Glucan Receptor
J. Exp. Med., December 1, 2003; 198(11): 1677 - 1688.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. D. Swain, S. J. Lee, M. C. Nussenzweig, and A. G. Harmsen
Absence of the Macrophage Mannose Receptor in Mice Does Not Increase Susceptibility to Pneumocystis carinii Infection In Vivo
Infect. Immun., November 1, 2003; 71(11): 6213 - 6221.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. E. Lasbury, X. Tang, P. J. Durant, and C.-H. Lee
Effect of Transcription Factor GATA-2 on Phagocytic Activity of Alveolar Macrophages from Pneumocystis carinii-Infected Hosts
Infect. Immun., September 1, 2003; 71(9): 4943 - 4952.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. E. Lund, K. Schuer, M. Hollifield, T. D. Randall, and B. A. Garvy
Clearance of Pneumocystis carinii in Mice Is Dependent on B Cells But Not on P. carinii-Specific Antibody
J. Immunol., August 1, 2003; 171(3): 1423 - 1430.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. P. Utz, A. H. Limper, S. Kalra, U. Specks, J. P. Scott, Z. Vuk-Pavlovic, and D. R. Schroeder
Etanercept for the Treatment of Stage II and III Progressive Pulmonary Sarcoidosis
Chest, July 1, 2003; 124(1): 177 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Lebron, R. Vassallo, V. Puri, and A. H. Limper
Pneumocystis carinii Cell Wall {beta}-Glucans Initiate Macrophage Inflammatory Responses through NF-{kappa}B Activation
J. Biol. Chem., June 27, 2003; 278(27): 25001 - 25008.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Y. Hahn, S. E. Evans, T. J. Kottom, J. E. Standing, R. E. Pagano, and A. H. Limper
Pneumocystis carinii Cell Wall beta -Glucan Induces Release of Macrophage Inflammatory Protein-2 from Alveolar Epithelial Cells via a Lactosylceramide-mediated Mechanism
J. Biol. Chem., January 10, 2003; 278(3): 2043 - 2050.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
C. Guyard, N. Seguy, J.-C. Cailliez, H. Drobecq, L. Polonelli, E. Dei-Cas, A. Mercenier, and F. D. Menozzi
Characterization of a Williopsis saturnus var. mrakii high molecular weight secreted killer toxin with broad-spectrum antimicrobial activity
J. Antimicrob. Chemother., June 1, 2002; 49(6): 961 - 971.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. J. Kottom, C. F. Thomas Jr., and A. H. Limper
Characterization of Pneumocystis carinii PHR1, a pH-Regulated Gene Important for Cell Wall Integrity
J. Bacteriol., December 1, 2001; 183(23): 6740 - 6745.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. Vassallo, T. J. Kottom, J. E. Standing, and A. H. Limper
Vitronectin and Fibronectin Function as Glucan Binding Proteins Augmenting Macrophage Responses to Pneumocystis carinii
Am. J. Respir. Cell Mol. Biol., August 1, 2001; 25(2): 203 - 211.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Vassallo, J. E. Standing, and A. H. Limper
Isolated Pneumocystis carinii Cell Wall Glucan Provokes Lower Respiratory Tract Inflammatory Responses
J. Immunol., April 1, 2000; 164(7): 3755 - 3763.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T.-J. Lin, N. Hirji, G. R. Stenton, M. Gilchrist, B. J. Grill, A. D. Schreiber, and A. D. Befus
Activation of Macrophage CD8: Pharmacological Studies of TNF and IL-1{beta} Production
J. Immunol., February 15, 2000; 164(4): 1783 - 1792.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. G. Rudmann, A. M. Preston, M. W. Moore, and J. M. Beck
Susceptibility to Pneumocystis carinii in Mice Is Dependent on Simultaneous Deletion of IFN-{gamma} and Type 1 and 2 TNF Receptor Genes
J. Immunol., July 1, 1998; 161(1): 360 - 366.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. J. Kottom and A. H. Limper
Cell Wall Assembly by Pneumocystis carinii. EVIDENCE FOR A UNIQUE Gsc-1 SUBUNIT MEDIATING beta -1,3-GLUCAN DEPOSITION
J. Biol. Chem., December 15, 2000; 275(51): 40628 - 40634.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.