The Journal of Immunology, Vol 150, Issue 8 3421-3426, Copyright © 1993 by American Association of Immunologists

ARTICLES

A monoclonal antibody produced during infection which recognizes an epitope of influenza hemagglutinin only in the context of H-2k MHC class I antigen

L McLain and NJ Dimmock
Department of Biological Sciences, University of Warwick, Coventry, UK.

A hybridoma prepared from cells isolated from the lungs of H-2k mice infected with influenza virus (A/WSN) produces a monoclonal IgM (LM41) which recognizes an epitope of the A/WSN hemagglutinin (HA) Ag only when it is expressed in H-2k cells, as shown by Ag binding and complement-mediated lysis. However, it did not bind to the native HA of virus particles or any form of HA protein present on A/WSN-infected H- 2d cells. LM41 inhibited Ag-specific, MHC class I-restricted lysis of H- 2k target cells infected with a vaccinia recombinant virus expressing the HA of A/WSN by CTL from A/WSN virus-infected H-2k mice. We conclude that LM41 has a similar Ag specificity to the HA-specific TCR.

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