The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Freedman, A. S.
Right arrow Articles by Vivier, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freedman, A. S.
Right arrow Articles by Vivier, E.

The Journal of Immunology, Vol 150, Issue 5 1645-1652, Copyright © 1993 by American Association of Immunologists

ARTICLES

Stimulation of protein tyrosine phosphorylation in human B cells after ligation of the beta 1 integrin VLA-4

AS Freedman, K Rhynhart, Y Nojima, J Svahn, L Eliseo, CD Benjamin, C Morimoto and E Vivier
Department of Medicine, Harvard Medical School, Boston, MA.

B lymphocytes express several adhesion molecules that are involved in cell-cell and cell-extracellular matrix interactions. The alpha 4 beta 1 integrin VLA-4, expressed on pre-B and mature/activated B cells, mediates adhesion of these cells to its two ligands, VCAM-1 and fibronectin. Recent evidence suggests that VLA-4 is involved in T lymphocyte activation; however, relatively little is known of the role of VLA-4 in B cell differentiation. To begin to assess the potential involvement of VLA-4 in B cell activation, we have examined the effect of ligation of VLA-4 on protein tyrosine phosphorylation in B cells. We found that cross-linking of VLA-4 by either mAb or natural ligands (i.e., VCAM-1 and the FN-40 cleavage fragment of fibronectin) induced the tyrosine phosphorylation of a 110-kDa protein in a human pre-B cell line (Nalm-6), an EBV-transformed B cell line (SB), and normal tonsillar B cells. These findings suggest that VLA-4 can activate a tyrosine kinase in B cells and B cell lines. These signals may be involved in the subsequent differentiation of pre-B and mature B cells within specific microenvironments where VLA-4 mediated adhesion is operational.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. W. K. Tse, M. Dang-Lawson, R. L. Lee, D. Vong, A. Bulic, L. Buckbinder, and M. R. Gold
B Cell Receptor-induced Phosphorylation of Pyk2 and Focal Adhesion Kinase Involves Integrins and the Rap GTPases and Is Required for B Cell Spreading
J. Biol. Chem., August 21, 2009; 284(34): 22865 - 22877.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Zhu, R. Garrett, Y. Jung, Y. Zhang, N. Kim, J. Wang, G. J. Joe, E. Hexner, Y. Choi, R. S. Taichman, et al.
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells
Blood, May 1, 2007; 109(9): 3706 - 3712.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
W. El Nemer, M.-P. Wautier, C. Rahuel, P. Gane, P. Hermand, F. Galacteros, J.-L. Wautier, J.-P. Cartron, Y. Colin, and C. Le Van Kim
Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell {alpha}4{beta}1integrin: role in adhesion of sickle red blood cells to endothelial cells
Blood, April 15, 2007; 109(8): 3544 - 3551.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
B. J. Holleran, E. Barbar, M. D. Payet, and G. Dupuis
Differential recruitment of {alpha}2{beta}1 and {alpha}4{beta}1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin
J. Leukoc. Biol., February 1, 2003; 73(2): 243 - 252.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. I. Reyes, P. Escobar, M. R. Bono, and M. Rosemblatt
Adhesion of B Cell Lines to Endothelial Cells from Human Lymphoid Tissue Modulates Tyrosine Phosphorylation and Endothelial Cell Activation
J. Immunol., November 15, 2002; 169(10): 5881 - 5888.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Yasuda, Y. Tanaka, M. Tamura, K. Fujii, M. Sugaya, T. So, M. Takenoyama, and K. Yasumoto
Stimulation of {beta}1 Integrin Down-Regulates ICAM-1 Expression and ICAM-1-dependent Adhesion of Lung Cancer Cells through Focal Adhesion Kinase
Cancer Res., March 1, 2001; 61(5): 2022 - 2030.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
H. Shenoi, J. Seavitt, A. Zheleznyak, M. L. Thomas, and E. J. Brown
Regulation of Integrin-Mediated T Cell Adhesion by the Transmembrane Protein Tyrosine Phosphatase CD45
J. Immunol., June 15, 1999; 162(12): 7120 - 7127.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Suen, D Ilic, E Caveggion, G Berton, C. Damsky, and C. Lowell
Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages
J. Cell Sci., January 11, 1999; 112(22): 4067 - 4078.
[Abstract] [PDF]

Home page
 JCBHome page
D. D. Chang, C. Wong, H. Smith, and J. Liu
ICAP-1, a Novel {beta}1 Integrin Cytoplasmic Domain-associated Protein, Binds to a Conserved and Functionally Important NPXY Sequence Motif of {beta}1 Integrin
J. Cell Biol., September 8, 1997; 138(5): 1149 - 1157.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. J. Jarvis, J. E. Maguire, and T. W. LeBien
Contact Between Human Bone Marrow Stromal Cells and B Lymphocytes Enhances Very Late Antigen-4/Vascular Cell Adhesion Molecule-1-Independent Tyrosine Phosphorylation of Focal Adhesion Kinase, Paxillin, and ERK2 in Stromal Cells
Blood, August 15, 1997; 90(4): 1626 - 1635.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. N. Manie, A. R.P. Beck, A. Astier, SusanF. Law, T. Canty, H. Hirai, B. J. Druker, H. Avraham, N. Haghayeghi, M. Sattler, et al.
Involvement of p130Cas and p105HEF1, a Novel Cas-like Docking Protein, in a Cytoskeleton-dependent Signaling Pathway Initiated by Ligation of Integrin or Antigen Receptor on Human B Cells
J. Biol. Chem., February 14, 1997; 272(7): 4230 - 4236.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Astier, H. Avraham, S. N. Manie, J. Groopman, T. Canty, S. Avraham, and A. S. Freedman
The Related Adhesion Focal Tyrosine Kinase Is Tyrosine-phosphorylated after beta 1-Integrin Stimulation in B Cells and Binds to p130cas
J. Biol. Chem., January 3, 1997; 272(1): 228 - 232.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Xiao, Y. Messinger, J. Jin, D. E. Myers, J. B. Bolen, and F. M. Uckun
Signal Transduction through the beta1 Integrin Family Surface Adhesion Molecules VLA-4 and VLA-5 of Human B-cell Precursors Activates CD19 Receptor-associated Protein-tyrosine Kinases
J. Biol. Chem., March 29, 1996; 271(13): 7659 - 7664.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
S. W. Hunt III, E. S. Harris, S.-A. Kellermann, and Y. Shimizu
T-Lymphocyte Interactions With Endothelium and Extracellular Matrix
Critical Reviews in Oral Biology & Medicine, January 1, 1996; 7(1): 59 - 86.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. R. E. Shaw, A. Domanska, A. Mak, A. Gilchrist, K. Dobler, L. Visser, S. Poppema, L. Fliegel, M. Letarte, and B. J. Willett
Ectopic Expression of Human and Feline CD9 in a Human B Cell Line Confers beta1 Integrin-dependent Motility on Fibronectin and Laminin Substrates and Enhanced Tyrosine Phosphorylation
J. Biol. Chem., October 13, 1995; 270(41): 24092 - 24099.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.