| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 150, Issue 4 1307-1313, Copyright © 1993 by American Association of Immunologists
ARTICLES |
JJ Campbell and Y Hashimoto
University of Pennsylvania, School of Medicine, Department of Pathology, Philadelphia 19104.
We have used a highly sensitive Northern blot probing technique to examine RAG-1 and RAG-2 mRNA expression in unmanipulated murine thymocytes of various developmental phenotypes sorted by flow cytometry. We have found that neither RAG-1 nor RAG-2 are down- regulated until thymocytes reach the TCRhi stage. TCRhi, CD8+CD4+ cells have lost RAG-2 mRNA but continue to express significant levels of RAG- 1 mRNA, providing molecular evidence that this cell type is transitional between the TCRlo, CD4+CD8+ and the TCRhi, CD4+CD8-, or CD4-CD8+ phenotypes.
This article has been cited by other articles:
|
|
 |
|
  N. Niederberger, L. K. Buehler, J. Ampudia, and N. R. J. Gascoigne Thymocyte stimulation by anti-TCR-{beta}, but not by anti-TCR-{alpha}, leads to induction of developmental transcription program J. Leukoc. Biol., May 1, 2005; 77(5): 830 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. E. Mick, T. K. Starr, T. M. McCaughtry, L. K. McNeil, and K. A. Hogquist The Regulated Expression of a Diverse Set of Genes during Thymocyte Positive Selection In Vivo J. Immunol., November 1, 2004; 173(9): 5434 - 5444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Puls, K. A. Hogquist, N. Reilly, and M. D. Wright CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics Int. Immunol., March 1, 2002; 14(3): 249 - 258. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
