The Journal of Immunology, Vol 150, Issue 3 748-752, Copyright © 1993 by American Association of Immunologists

ARTICLES

Activity of stem cell factor and IL-7 in combination on normal bone marrow B lineage cells

PE Funk, A Varas and PL Witte
Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153.

The production of B cells is regulated by soluble and cell contact signals presumably provided by bone marrow stromal cells. Among these is IL-7, a well characterized proliferative stimulus for a subset of pre-B cells. Stem cell factor (SCF), a stromal cell-derived cytokine with broad hemopoietic effects, has been reported to synergize with IL- 7 to drive the proliferation and differentiation of B220- bone marrow cells into B220+ B cell precursors in long term culture. A subsequent report has cast doubt on this result by showing that SCF and IL-7 were incapable of producing mu+ pre-B cells after short term culture. Here, using the cell sorter to assure discrete separation of B220+ and B220- cells followed by soft agar culture to prevent interaction with accessory cells, we demonstrate that the combination of SCF and IL-7 does not stimulate the expansion or differentiation of B220- lymphoid precursors but can act synergistically in the clonal proliferation of B220+ cells.

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