The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fonteh, A. N.
Right arrow Articles by Chilton, F. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fonteh, A. N.
Right arrow Articles by Chilton, F. H.

The Journal of Immunology, Vol 150, Issue 2 563-570, Copyright © 1993 by American Association of Immunologists

ARTICLES

Mobilization of different arachidonate pools and their roles in the generation of leukotrienes and free arachidonic acid during immunologic activation of mast cells

AN Fonteh and FH Chilton
Bowman Gray School of Medicine of Wake Forest University, Winston- Salem, NC 27157-1054.

Immunologic activation of mast cells leads to the mobilization of arachidonic acid (AA) from membrane phospholipids and the subsequent conversion of this AA to bioactive products. The objective of our study was to determine if segregated pools of AA-containing phospholipids within mast cells serve as independent sources of AA. Initial studies indicated that the appearance of free AA occurred rapidly (maximal level formed within 1 min) within supernatant fluids of Ag-stimulated mast cells and was kinetically different from the formation of leukotriene (LT) B4 or LTC4. To examine whether free AA and leukotrienes were mobilized from different sources, AA-containing phospholipids of mast cells were labeled with [14C] and [3H] AA such that all major subclasses (1-acyl-1-alkyl-1-alk-1'-enyl) of phospholipids contained different ratios of [3H] to [14C] (sp. act. ratios (SAR)). Mast cells were then stimulated with Ag and the SAR of cellular AA, extracellular AA and extracellular LTC4, LTB4, 6-trans LTB4, were determined. The SAR were uniform in all LT and mimicked the ratio found in cellular AA. By contrast, the SAR of AA released into supernatant fluids was twofold lower than that of LT. This indicated that AA released as free fatty acid clearly was derived from a different lipid pool than AA that formed LT. Although it was apparent that the pools which gave rise to AA and LT were different, defining phospholipid(s) that constitute these distinct pools proved more difficult. The SAR of LT suggested that their cellular precursor AA could have been derived from several phospholipid subclasses; however, the SAR in phosphatidylcholine and phosphatidylinositol most closely matched the LT. The SAR of AA in supernatant fluids implied that it was derived, in part, from phosphatidylethanolamine subclasses. Taken together, these data suggest that there are at least two different pools of AA that are mobilized in mast cells during Ag activation.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. N. Fonteh, G.-i. Atsumi, T. LaPorte, and F. H. Chilton
Secretory Phospholipase A2 Receptor-Mediated Activation of Cytosolic Phospholipase A2 in Murine Bone Marrow-Derived Mast Cells
J. Immunol., September 1, 2000; 165(5): 2773 - 2782.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. M. Johnson, B. Vaughn, M. Triggiani, D. D. Swan, A. N. Fonteh, and F. H. Chilton
Role of Arachidonyl Triglycerides within Lipid Bodies in Eicosanoid Formation by Human Polymorphonuclear Cells
Am. J. Respir. Cell Mol. Biol., August 1, 1999; 21(2): 253 - 258.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. G. Obukowicz, D. J. Welsch, W. J. Salsgiver, C. L. Martin-Berger, K. S. Chinn, K. L. Duffin, A. Raz, and P. Needleman
Novel, Selective Delta 6 or Delta 5 Fatty Acid Desaturase Inhibitors as Antiinflammatory Agents in Mice
J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 157 - 166.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. Wu, C. W. Angus, X.-L. Yao, C. Logun, and J. H. Shelhamer
p11, a Unique Member of the S100 Family of Calcium-binding Proteins, Interacts with and Inhibits the Activity of the 85-kDa Cytosolic Phospholipase A2
J. Biol. Chem., July 4, 1997; 272(27): 17145 - 17153.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Balsinde and E. A. Dennis
Function and Inhibition of Intracellular Calcium-independent Phospholipase A2
J. Biol. Chem., June 27, 1997; 272(26): 16069 - 16072.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Krischer, M. Eisenmann, A. Bock, and M. J. Mueller
Protein-facilitated Export of Arachidonic Acid from Pig Neutrophils
J. Biol. Chem., April 18, 1997; 272(16): 10601 - 10607.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. T. Reddy and H. R. Herschman
Prostaglandin Synthase-1 and Prostaglandin Synthase-2 Are Coupled to Distinct Phospholipases for the Generation of Prostaglandin D2 in Activated Mast Cells
J. Biol. Chem., February 7, 1997; 272(6): 3231 - 3237.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.ús Balsinde and E. A. Dennis
Distinct Roles in Signal Transduction for Each of the Phospholipase A(2) Enzymes Present in P388D(1) Macrophages
J. Biol. Chem., March 22, 1996; 271(12): 6758 - 6765.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. N. Fonteh, T. LaPorte, D. Swan, and M. A. McAlexander
A Decrease in Remodeling Accounts for the Accumulation of Arachidonic Acid in Murine Mast Cells Undergoing Apoptosis
J. Biol. Chem., January 5, 2001; 276(2): 1439 - 1449.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1993 by The American Association of Immunologists, Inc. All rights reserved.