| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 150, Issue 10 4395-4406, Copyright © 1993 by American Association of Immunologists
ARTICLES |
M Martin, A Strasser, N Baumgarth, FM Cicuttini, K Welch, E Salvaris and AW Boyd
Lions Laboratory, Royal Melbourne Hospital, Victoria, Australia.
The suspension pro granulocyte/macrophage (SPGM1) cell line was established from a transplantable mouse progranulocytic/promacrophage tumor. Surprisingly, SPGM1 cells expressed a typical CD5 pre-B cell phenotype, being positive for Ly-1 (CD5), PB76, B220 (CD45RA), and the pre-B Ig receptor complex (microH chains, lambda 5 and vpre-B surrogate L chains, and the IgM alpha (mb-1) and Ig beta (B29) co-receptor molecules). Southern Blot analysis revealed clonal rearrangement of the microH chain locus and germ-line L chain loci. SPGM1 formed blast cell- , macrophage-, and occasional granulocytic colonies in soft agar in the presence of murine IL-3. IL-3 also induced macrophage differentiation of SPGM1 cells in suspension cultures. The earliest changes were detectable at 24 h by Northern blot analysis. IL-3-treatment increased Mac1 mRNA, induced c-fms mRNA, and down-regulated mRNA for mu, lambda 5, vpre-B and mb-1. After 2 to 4 days the cells were larger, strongly adherent, expressed the macrophage markers Mac1 and F4/80, had lost microH chain and PB76 surface expression, and readily phagocytosed latex beads. Thus SPGM1 has all the characteristic features of a CD5+ pre-B cell line. However, IL-3 predominantly induced SPGM1 to switch its differentiation program from a pre-B cell to a macrophage. This inducible, rapid switch of virtually the entire population provides a unique model for the molecular analysis of mechanisms involved in cell- fate determination.
This article has been cited by other articles:
|
|
 |
|
  S. Hodawadekar, F. Wei, D. Yu, A. Thomas-Tikhonenko, and M. L. Atchison Epigenetic Histone Modifications Do Not Control Ig{kappa} Locus Contraction and Intranuclear Localization in Cells with Dual B Cell-Macrophage Potential J. Immunol., November 1, 2006; 177(9): 6165 - 6171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Taghon, E.-S. David, J. C. Zuniga-Pflucker, and E. V. Rothenberg Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling Genes & Dev., April 15, 2005; 19(8): 965 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chalandon, X. Jiang, G. Hazlewood, S. Loutet, E. Conneally, A. Eaves, and C. Eaves Modulation of p210BCR-ABL activity in transduced primary human hematopoietic cells controls lineage programming Blood, May 1, 2002; 99(9): 3197 - 3204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lu, C. A. Bonham, X. Liang, Z. Chen, W. Li, L. Wang, S. C. Watkins, M. A. Nalesnik, M. S. Schlissel, A. J. Demestris, et al. Liver-Derived DEC205+B220+CD19- Dendritic Cells Regulate T Cell Responses J. Immunol., June 15, 2001; 166(12): 7042 - 7052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Chiang and J. G. Monroe Role for Transcription Pax5A Factor in Maintaining Commitment to the B Cell Lineage by Selective Inhibition of Granulocyte-Macrophage Colony-Stimulating Factor Receptor Expression J. Immunol., May 15, 2001; 166(10): 6091 - 6098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Chiang and J. G. Monroe BSAP/Pax5A Expression Blocks Survival and Expansion of Early Myeloid Cells Implicating Its Involvement in Maintaining Commitment to the B-Lymphocyte Lineage Blood, December 1, 1999; 94(11): 3621 - 3632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Borrello and R. P. Phipps Fibroblast-Secreted Macrophage Colony-Stimulating Factor Is Responsible for Generation of Biphenotypic B/Macrophage Cells from a Subset of Mouse B Lymphocytes J. Immunol., October 1, 1999; 163(7): 3605 - 3611. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
