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On the Anti-Hemolytic Action of Hemolytic Immune Sera

From the Clinical Laboratory of Dr. T. Kumagai of the Medical Department of Tohoku Imperial University, Sendai, Japan

Abstract

I. Introduction. Since Ehrlich (1) in 1898 discovered the inhibition of the hemolytic action of tetanus-toxin by normal horse serum, many investigations of anti-hemolytic action have been made.

Kraus and Clairmont (2) and Eisler (3) found that normal horse serum could inhibit the hemolytic action not only of tetanus toxin, but of staphylococci, streptococci, vibrios, and putrefactive bacilli.

Ranson (4) examining the hemolytic action of saponin against dog's blood corpuscles, discovered that the addition of dog's serum to saponin would inhibit its hemolytic action.

Human serum also has an anti-hemolytic action. But it was due to the discovery of Neisser and Doering (5), Neisser and Friedemann (6), and Senator (7) that the fact that such action of serum was possible only when it was inactivated by being heated to 56°C. became known.

Much (8) reported that the serum of subjects of psychosis has the power of inhibiting the hemolytic action of cobra toxin against human blood corpuscles.