The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Descoteaux, A.
Right arrow Articles by Turco, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Descoteaux, A.
Right arrow Articles by Turco, S. J.

The Journal of Immunology, Vol 149, Issue 9 3008-3015, Copyright © 1992 by American Association of Immunologists

ARTICLES

Inhibition of macrophage protein kinase C-mediated protein phosphorylation by Leishmania donovani lipophosphoglycan

A Descoteaux, G Matlashewski and SJ Turco
Department of Biochemistry, University of Kentucky Medical Center, Lexington 40536.

The cell surface lipophosphoglycan (LPG) from Leishmania donovani promastigotes is a potent inhibitor of purified protein kinase C (PKC) activity in vitro. In this study, we have investigated the effect of LPG on the activation process of PKC in murine bone marrow-derived macrophages. The extent and kinetics of calcium ionophore A23187- induced [3H] phorbol dibutyrate binding to macrophages were not affected by LPG pretreatment or infection with either wild-type or LPG- deficient promastigotes, indicating no effect on the association of calcium-dependent PKC with the plasma membrane. In contrast, LPG inhibited the phosphorylation of both the PKC-specific VRKRTRLLR substrate peptide and MARCKS, and endogenous PKC substrate, in 1-oleoyl- 2-acetyl-glycerol-stimulated macrophages. These observations provide direct evidence that LPG effectively inhibits PKC activity in intact macrophages. Finally, depletion of PKC rendered macrophages more permissive for the proliferation of L. donovani, suggesting that inhibition of PKC-dependent events contributes to the survival of this parasite within its host cell.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
U. M. Padigel, J. J. Lee, T. J. Nolan, G. A. Schad, and D. Abraham
Eosinophils Can Function as Antigen-Presenting Cells To Induce Primary and Secondary Immune Responses to Strongyloides stercoralis.
Infect. Immun., June 1, 2006; 74(6): 3232 - 3238.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Dey, A. Sarkar, N. Majumder, S. Bhattacharyya (Majumdar), K. Roychoudhury, S. Bhattacharyya, S. Roy, and S. Majumdar
Regulation of Impaired Protein Kinase C Signaling by Chemokines in Murine Macrophages during Visceral Leishmaniasis
Infect. Immun., December 1, 2005; 73(12): 8334 - 8344.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
M. Olivier, D. J. Gregory, and G. Forget
Subversion Mechanisms by Which Leishmania Parasites Can Escape the Host Immune Response: a Signaling Point of View
Clin. Microbiol. Rev., April 1, 2005; 18(2): 293 - 305.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. Guizani-Tabbane, K. Ben-Aissa, M. Belghith, A. Sassi, and K. Dellagi
Leishmania major Amastigotes Induce p50/c-Rel NF-{kappa}B Transcription Factor in Human Macrophages: Involvement in Cytokine Synthesis
Infect. Immun., May 1, 2004; 72(5): 2582 - 2589.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
B. L. Kelly, D. B. Stetson, and R. M. Locksley
Leishmania major LACK Antigen Is Required for Efficient Vertebrate Parasitization
J. Exp. Med., December 1, 2003; 198(11): 1689 - 1698.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Mookerjee, P. C. Sen, and A. C. Ghose
Immunosuppression in Hamsters with Progressive Visceral Leishmaniasis Is Associated with an Impairment of Protein Kinase C Activity in Their Lymphocytes That Can Be Partially Reversed by Okadaic Acid or Anti-Transforming Growth Factor {beta} Antibody
Infect. Immun., May 1, 2003; 71(5): 2439 - 2446.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. Chawla and R. A. Vishwakarma
Alkylacylglycerolipid domain of GPI molecules of Leishmania is responsible for inhibition of PKC-mediated c-fos expression
J. Lipid Res., March 1, 2003; 44(3): 594 - 600.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. R. Gantt, T. L. Goldman, M. L. McCormick, M. A. Miller, S. M. B. Jeronimo, E. T. Nascimento, B. E. Britigan, and M. E. Wilson
Oxidative Responses of Human and Murine Macrophages During Phagocytosis of Leishmania chagasi
J. Immunol., July 15, 2001; 167(2): 893 - 901.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. S. Campos, I. C. Almeida, O. Takeuchi, S. Akira, E. P. Valente, D. O. Procopio, L. R. Travassos, J. A. Smith, D. T. Golenbock, and R. T. Gazzinelli
Activation of Toll-Like Receptor-2 by Glycosylphosphatidylinositol Anchors from a Protozoan Parasite
J. Immunol., July 1, 2001; 167(1): 416 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Bhattacharyya, S. Ghosh, P. L. Jhonson, S. K. Bhattacharya, and S. Majumdar
Immunomodulatory Role of Interleukin-10 in Visceral Leishmaniasis: Defective Activation of Protein Kinase C-Mediated Signal Transduction Events
Infect. Immun., March 1, 2001; 69(3): 1499 - 1507.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Buates and G. Matlashewski
General Suppression of Macrophage Gene Expression During Leishmania donovani Infection
J. Immunol., March 1, 2001; 166(5): 3416 - 3422.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. L. Sacks, G. Modi, E. Rowton, G. Spath, L. Epstein, S. J. Turco, and S. M. Beverley
The role of phosphoglycans in Leishmania-sand fly interactions
PNAS, January 4, 2000; 97(1): 406 - 411.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. St-Denis, V. Caouras, F. Gervais, and A. Descoteaux
Role of Protein Kinase C-{alpha} in the Control of Infection by Intracellular Pathogens in Macrophages
J. Immunol., November 15, 1999; 163(10): 5505 - 5511.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Corradin, J. Mauel, A. Ransijn, C. Sturzinger, and G. Vergeres
Down-regulation of MARCKS-related Protein (MRP) in Macrophages Infected with Leishmania
J. Biol. Chem., June 11, 1999; 274(24): 16782 - 16787.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Pingel, Z.-E. Wang, and R. M. Locksley
Distribution of Protein Kinase C Isoforms after Infection of Macrophages with Leishmania major
Infect. Immun., April 1, 1998; 66(4): 1795 - 1799.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
M. Desjardins and A. Descoteaux
Inhibition of Phagolysosomal Biogenesis by the Leishmania Lipophosphoglycan
J. Exp. Med., June 16, 1997; 185(12): 2061 - 2068.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Galley, G. Hagens, I. Glaser, W. Davis, M. Eichhorn, and D. Dobbelaere
Jun NH2-terminal kinase is constitutively activated in T cells transformed by the intracellular parasite Theileria parva
PNAS, May 13, 1997; 94(10): 5119 - 5124.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. S. Hall, O. O. Daramola, G. Barden, and G. A.T. Targett
Modulation of Protein Kinase C Activity in Plasmodium falciparum - Infected Erythrocytes
Blood, March 1, 1997; 89(5): 1770 - 1778.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Miller, S. E. McGowan, K. R. Gantt, M. Champion, S. L. Novick, K. A. Andersen, C. J. Bacchi, N. Yarlett, B. E. Britigan, and M. E. Wilson
Inducible Resistance to Oxidant Stress in the Protozoan Leishmania chagasi
J. Biol. Chem., October 20, 2000; 275(43): 33883 - 33889.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1992 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1992 by The American Association of Immunologists, Inc. All rights reserved.