| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 149, Issue 5 1758-1762, Copyright © 1992 by American Association of Immunologists
ARTICLES |
K Shibuya, T Abe and T Fujita
Institute of Clinical Medicine, University of Tsukuba, Japan.
Decay-accelerating factor (DAF) is a glycosylphosphatidylinositol- anchored membrane protein that protects cells from damage by autologous complement activation. Of the four mAb against DAF prepared in our laboratory, 1C6 completely blocked DAF function, whereas 5B2 partially blocked it. Using these mAb, we investigated whether human monocytes were activated via DAF molecules. When monocytes were incubated with 1C6 alone, glucose was consumed in significant amounts and phagocytosis of latex beads was enhanced, indicating that the monocytes had been activated. However, 1C6 did not enhance the production of monokines, TNF-alpha, and IL-1 alpha and -beta. The F(ab')2 fragment of 1C6 also activated monocytes, whereas 5B2 and the Fab fragment of 1C6 could not. To further examine monocyte activation, these cells were treated with phosphatidylinositol-specific phospholipase C. Increased glucose consumption and enhanced phagocytic activity by 1C6 were considerably reduced in monocytes treated with phosphatidylinositol-specific phospholipase C. In addition, we found that 1C6 stimulated the generation of inositol trisphosphate. These results demonstrate that the signal transmitted via the DAF molecule is capable of stimulating monocytes.
This article has been cited by other articles:
|
|
 |
|
  K. G.N. Suzuki, T. K. Fujiwara, M. Edidin, and A. Kusumi Dynamic recruitment of phospholipase C{gamma} at transiently immobilized GPI-anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2 J. Cell Biol., May 21, 2007; 177(4): 731 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Cauvi, G. Cauvi, and K. M. Pollard Constitutive Expression of Murine Decay-Accelerating Factor 1 Is Controlled by the Transcription Factor Sp1 J. Immunol., September 15, 2006; 177(6): 3837 - 3847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Servin Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli Clin. Microbiol. Rev., April 1, 2005; 18(2): 264 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kuraya, M. Matsushita, Y. Endo, S. Thiel, and T. Fujita Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G Int. Immunol., January 1, 2003; 15(1): 109 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Caragine, M. Imai, A. B. Frey, and S. Tomlinson Expression of rat complement control protein Crry on tumor cells inhibits rat natural killer cell-mediated cytotoxicity Blood, October 16, 2002; 100(9): 3304 - 3310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Heine, A.J. Ulmer, V.T. El-Samalouti, A. Lentschat, and L. Hamann Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex Innate Immunity, June 1, 2001; 7(3): 227 - 231. [Abstract] [PDF]
|
|
|
|
 |
|
 I. Peiffer, A. L. Servin, and M.-F. Bernet-Camard Piracy of Decay-Accelerating Factor (CD55) Signal Transduction by the Diffusely Adhering Strain Escherichia coli C1845 Promotes Cytoskeletal F-Actin Rearrangements in Cultured Human Intestinal INT407 Cells Infect. Immun., September 1, 1998; 66(9): 4036 - 4042. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
