The Journal of Immunology, Vol 149, Issue 12 3990-3994, Copyright © 1992 by American Association of Immunologists

ARTICLES

Highly diverse T cell recognition of a single Plasmodium berghei peptide presented by a series of mutant H-2Kd molecules

C Jaulin, JL Casanova, P Romero, I Luescher, AS Cordey, JL Maryanski and P Kourilsky
Unite de Biologie Moleculaire du Gene, Unite INSERM 277, Institut Pasteur, Paris, France.

We have tested 21 independent CTL clones for recognition of a single peptide derived from the Plasmodium berghei circumsporozoite protein in the context of 13 mutants of the murine MHC class I molecule H-2Kd. In this series of Kd mutants, amino acid residues located on the upper surface of the alpha-helices were individually substituted by alanine. Remarkably, most clones displayed individual recognition patterns on the Kd mutants. We had previously found that this series of CTL clones was likewise highly diverse in terms of both TCR primary structure and peptide fine specificity. Our data thus reinforce the concept that multiple T cell epitopes are available on the surface of a single peptide-MHC class I complex for recognition by specific TCR.

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