The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Newman, M. A.
Right arrow Articles by Smith-Gill, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Newman, M. A.
Right arrow Articles by Smith-Gill, S. J.

The Journal of Immunology, Vol 149, Issue 10 3260-3272, Copyright © 1992 by American Association of Immunologists

ARTICLES

Patterns of antibody specificity during the BALB/c immune response to hen eggwhite lysozyme

MA Newman, CR Mainhart, CP Mallett, TB Lavoie and SJ Smith-Gill
Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

We tested 49 BALB/c antilysozyme mAb from seven intervals during the immune response to lysozyme for patterns of specificity and avidity. We found that the antibody epitopes in composite covered at least 80% of the lysozyme surface, and their patterns of overlap suggest a continuum of potential antibody epitopes. Previously observed regional specificities, which emerged at different times in the immune response, were more discretely defined in late response antibodies, when the majority of mAb could be assigned to one of three functionally nonoverlapping complementation groups. The area covered by each antigenic region may be greater than an individual epitope, and may include multiple epitopes that overlap structurally and functionally to varying degrees. Connectivity between antigenic regions was seen in interactions among early and late stage antibodies, and among secondary stage mAb, but not among tertiary stage mAb from hyperimmunized mice. Patterns of overlap of early and late response antibodies suggest that the organization of antibody specificities change during the progression from primary to secondary to tertiary response. Over the same period in the response, the average relative avidity of IgG1 kappa mAb did not increase, suggesting that "affinity maturation" of serum antibodies reflects an increase in the number and diversity of antibodies, rather than an overall increase in the avidity of individual antibodies.


This article has been cited by other articles:


Home page
 Protein Eng Des SelHome page
H. Persson, H. Wallmark, A. Ljungars, J. Hallborn, and M. Ohlin
In vitro evolution of an antibody fragment population to find high-affinity hapten binders
Protein Eng. Des. Sel., August 1, 2008; 21(8): 485 - 493.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Breithaupt, B. Schafer, H. Pellkofer, R. Huber, C. Linington, and U. Jacob
Demyelinating Myelin Oligodendrocyte Glycoprotein-Specific Autoantibody Response Is Focused on One Dominant Conformational Epitope Region in Rodents
J. Immunol., July 15, 2008; 181(2): 1255 - 1263.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. R. Poulsen, P.-J. Meijer, A. Jensen, L. S. Nielsen, and P. S. Andersen
Kinetic, Affinity, and Diversity Limits of Human Polyclonal Antibody Responses against Tetanus Toxoid
J. Immunol., September 15, 2007; 179(6): 3841 - 3850.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
D. Paus, T. G. Phan, T. D. Chan, S. Gardam, A. Basten, and R. Brink
Antigen recognition strength regulates the choice between extrafollicular plasma cell and germinal center B cell differentiation
J. Exp. Med., April 17, 2006; 203(4): 1081 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ho, R. J. Kreitman, M. Onda, and I. Pastan
In Vitro Antibody Evolution Targeting Germline Hot Spots to Increase Activity of an Anti-CD22 Immunotoxin
J. Biol. Chem., January 7, 2005; 280(1): 607 - 617.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Cauerhff, F. A. Goldbaum, and B. C. Braden
Structural mechanism for affinity maturation of an anti-lysozyme antibody
PNAS, March 9, 2004; 101(10): 3539 - 3544.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Mohan, N. Sinha, and S. J. Smith-Gill
Modeling the Binding Sites of Anti-Hen Egg White Lysozyme Antibodies HyHEL-8 and HyHEL-26: An Insight into the Molecular Basis of Antibody Cross-Reactivity and Specificity
Biophys. J., November 1, 2003; 85(5): 3221 - 3236.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
N. Sinha, S. Mohan, C. A. Lipschultz, and S. J. Smith-Gill
Differences in Electrostatic Properties at Antibody-Antigen Binding Sites: Implications for Specificity and Cross-Reactivity
Biophys. J., December 1, 2002; 83(6): 2946 - 2968.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Lu and R. S. Hodges
A de Novo Designed Template for Generating Conformation-specific Antibodies That Recognize alpha -Helices in Proteins
J. Biol. Chem., June 21, 2002; 277(26): 23515 - 23524.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. M. Zinkernagel and H. Hengartner
Regulation of the Immune Response by Antigen
Science, July 13, 2001; 293(5528): 251 - 253.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
T. P. King, S. Y. Jim, R. I. Monsalve, A. Kagey-Sobotka, L. M. Lichtenstein, and M. D. Spangfort
Recombinant Allergens with Reduced Allergenicity but Retaining Immunogenicity of the Natural Allergens: Hybrids of Yellow Jacket and Paper Wasp Venom Allergen Antigen 5s
J. Immunol., May 15, 2001; 166(10): 6057 - 6065.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
M. Brown, M. A. Schumacher, G. D. Wiens, R. G. Brennan, and M. B. Rittenberg
The Structural Basis of Repertoire Shift in an Immune Response to Phosphocholine
J. Exp. Med., June 19, 2000; 191(12): 2101 - 2112.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. A. Goldbaum, A. Cauerhff, C. A. Velikovsky, A. S. Llera, M.-M. Riottot, and R. J. Poljak
Lack of Significant Differences in Association Rates and Affinities of Antibodies from Short-Term and Long-Term Responses to Hen Egg Lysozyme
J. Immunol., May 15, 1999; 162(10): 6040 - 6045.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. C. Jaume, J. Guo, D. L. Pauls, M. Zakarija, J. M. McKenzie, J. A. Egeland, C. L. Burek, N. R. Rose, W. H. Hoffman, B. Rapoport, et al.
Evidence for Genetic Transmission of Thyroid Peroxidase Autoantibody Epitopic "Fingerprints"
J. Clin. Endocrinol. Metab., April 1, 1999; 84(4): 1424 - 1431.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
P. England, R. Nageotte, M. Renard, A.-L. Page, and H. Bedouelle
Functional Characterization of the Somatic Hypermutation Process Leading to Antibody D1.3, a High Affinity Antibody Directed Against Lysozyme
J. Immunol., February 15, 1999; 162(4): 2129 - 2136.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Guermonprez, P. England, H. Bedouelle, and C. Leclerc
The Rate of Dissociation Between Antibody and Antigen Determines the Efficiency of Antibody-Mediated Antigen Presentation to T Cells
J. Immunol., November 1, 1998; 161(9): 4542 - 4548.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Jenne, K. Brepoels, D. Collen, and L. Jespers
High Resolution Mapping of the B Cell Epitopes of Staphylokinase in Humans Using Negative Selection of a Phage-Displayed Antigen Library
J. Immunol., September 15, 1998; 161(6): 3161 - 3168.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1992 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1992 by The American Association of Immunologists, Inc. All rights reserved.