The Journal of Immunology, Vol 148, Issue 8 2600-2608, Copyright © 1992 by American Association of Immunologists

ARTICLES

Identification and molecular cloning of tactile. A novel human T cell activation antigen that is a member of the Ig gene superfamily

PL Wang, S O'Farrell, C Clayberger and AM Krensky
Department of Pediatrics, Stanford University School of Medicine, CA 94305.

We have identified and cloned cDNA for a novel cell-surface protein that we have named Tactile for T cell activation, increased late expression. It is expressed on normal T cell lines and clones, and some transformed T cells, but no other cultured cell lines tested. It is expressed at low levels on peripheral T cells and is strongly up- regulated after activation, peaking 6 to 9 days after the activating stimulus. It is also up-regulated on NK cells activated in allogeneic cultures. It is not found on peripheral B cells but is expressed at very low levels on activated B cells. Tactile-specific mAb immunoprecipitates a band of 160 kDa when reduced and bands of 240, 180, and 160 kDa nonreduced. Using an antiserum produced with affinity- purified Tactile protein to screen a lambda gt11 library, we have identified Tactile cDNA. Northern blot analysis shows an expression pattern similar to that of the protein and transfection of COS cells with the full-length 5.2-kb cDNA results in cell-surface expression. Comparison with the sequence databanks show that Tactile is a member of the immunoglobulin gene superfamily, with similarity to Drosophila amalgam, the melanoma Ag MUC-18, members of the carcinoembryonic Ag family, the poliovirus receptor, and the neural cell adhesion molecule. The deduced primary sequence encodes a protein with three Ig domains, a long serine/threonine/proline-rich region typical of an extensively O- glycosylated domain, a transmembrane domain, and a 45 residue cytoplasmic domain. These data suggest that Tactile may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response.

This article has been cited by other articles:

				N. Hosen, C. Y. Park, N. Tatsumi, Y. Oji, H. Sugiyama, M. Gramatzki, A. M. Krensky, and I. L. Weissman CD96 is a leukemic stem cell-specific marker in human acute myeloid leukemia PNAS, June 26, 2007; 104(26): 11008 - 11013. [Abstract] [Full Text] [PDF]

				M. Carlsten, N. K. Bjorkstrom, H. Norell, Y. Bryceson, T. van Hall, B. C. Baumann, M. Hanson, K. Schedvins, R. Kiessling, H.-G. Ljunggren, et al. DNAX Accessory Molecule-1 Mediated Recognition of Freshly Isolated Ovarian Carcinoma by Resting Natural Killer Cells Cancer Res., February 1, 2007; 67(3): 1317 - 1325. [Abstract] [Full Text] [PDF]

				Y.-T. Ahn, B. Huang, L. McPherson, C. Clayberger, and A. M. Krensky Dynamic Interplay of Transcriptional Machinery and Chromatin Regulates "Late" Expression of the Chemokine RANTES in T Lymphocytes Mol. Cell. Biol., January 1, 2007; 27(1): 253 - 266. [Abstract] [Full Text] [PDF]

				S. Tahara-Hanaoka, K. Shibuya, H. Kai, A. Miyamoto, Y. Morikawa, N. Ohkochi, S.-i. Honda, and A. Shibuya Tumor rejection by the poliovirus receptor family ligands of the DNAM-1 (CD226) receptor Blood, February 15, 2006; 107(4): 1491 - 1496. [Abstract] [Full Text] [PDF]

				K. S. Boles, W. Barchet, T. Diacovo, M. Cella, and M. Colonna The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM Blood, August 1, 2005; 106(3): 779 - 786. [Abstract] [Full Text] [PDF]

				A. Fuchs, M. Cella, E. Giurisato, A. S. Shaw, and M. Colonna Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155) J. Immunol., April 1, 2004; 172(7): 3994 - 3998. [Abstract] [Full Text] [PDF]

				R. Bennett , Jr., T. Järvelä, P. Engelhardt, L. Kostamovaara, P. Sparks, O. Carpén, O. Turunen, and A. Vaheri Mucin MUC1 Is Seen in Cell Surface Protrusions Together with Ezrin in Immunoelectron Tomography and is Concentrated at Tips of Filopodial Protrusions in MCF-7 Breast Carcinoma Cells J. Histochem. Cytochem., January 1, 2001; 49(1): 67 - 78. [Abstract] [Full Text]

				S. M. Watt, L. H. Butler, M. Tavian, H.-J. Buhring, I. Rappold, P. J. Simmons, A. C. W. Zannettino, D. Buck, A. Fuchs, R. Doyonnas, et al. Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny but display distinct distribution patterns in adult hematopoietic and nonhematopoietic tissues Blood, May 15, 2000; 95(10): 3113 - 3124. [Abstract] [Full Text] [PDF]

				P. D. Sherrington, J. L. Scott, B. Jin, D. Simmons, D. J. Dorahy, J. Lloyd, J. H. Brien, R. H. Aebersold, J. Adamson, M. Zuzel, et al. TLiSA1 (PTA1) Activation Antigen Implicated in T Cell Differentiation and Platelet Activation Is a Member of the Immunoglobulin Superfamily Exhibiting Distinctive Regulation of Expression J. Biol. Chem., August 29, 1997; 272(35): 21735 - 21744. [Abstract] [Full Text] [PDF]