The Journal of Immunology, Vol 148, Issue 4 1086-1092, Copyright © 1992 by American Association of Immunologists

ARTICLES

IL-4 induces adherence of human eosinophils and basophils but not neutrophils to endothelium. Association with expression of VCAM-1

RP Schleimer, SA Sterbinsky, J Kaiser, CA Bickel, DA Klunk, K Tomioka, W Newman, FW Luscinskas, MA Gimbrone Jr and BW McIntyre
Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224.

The present studies were performed to explore potentially selective mechanisms of leukocyte adhesion in an attempt to understand how preferential recruitment of eosinophils and basophils might occur during allergic and other inflammatory reactions. Stimulation of human vascular endothelial cells for 24 h with IL-4 (30 to 1,000 U/ml) induced adhesion for eosinophils (up to approximately four-fold of control) and basophils (up to approximately twofold of control) but not neutrophils (less than 125% of control). Analysis of endothelial expression of adhesion molecules by flow cytometry revealed that IL-4 treatment induced vascular cell adhesion molecule-1 (VCAM-1) expression without significantly affecting the expression of other adhesion molecules, namely endothelial-leukocyte adhesion molecule-1 (ELAM-1) or intercellular adhesion molecule-1 (ICAM-1). The concentration-response curve for IL-4-induced VCAM-1 expression paralleled that for adhesion. Endothelial cells stimulated with IL-4 expressed adhesive properties for eosinophils by 3 h; the response increased steadily during a 24-h time course study. Eosinophils and basophils adhered to plates coated with a recombinant form of VCAM-1. This adhesion was blocked with antibodies to VCAM-1 but not ELAM-1. mAb directed against either VCAM-1 or VLA-4 inhibited (by approximately 75%) the binding of eosinophils and basophils to IL-4-stimulated endothelial cells. Because VLA-4 and VCAM-1 have been demonstrated to bind to each other in other adhesion systems, these results suggest that IL-4 stimulates eosinophil and basophil adhesion by inducing endothelial cell expression of VCAM-1 which binds to eosinophil and basophil VLA-4. The lack of expression of VLA-4 on neutrophils and the failure of IL-4 to stimulate neutrophil adherence support this conclusion. It is proposed that local release of IL-4 in vivo in allergic diseases or after experimental allergen challenge may partly explain the enrichment of eosinophils and basophils (vs neutrophils) observed in these situations.

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				Y. Ohashi, Y. Nakai, A. Tanaka, Y. Kakinoki, Y. Washio, A. Kato, T. Masamoto, K. Yamada, and M. Hayashi Serologic Study of the Working Mechanisms of Immunotherapy for Children With Perennial Allergic Rhinitis Arch Otolaryngol Head Neck Surg, December 1, 1998; 124(12): 1337 - 1346. [Abstract] [Full Text] [PDF]

				V. Patella, A. Giuliano, J.-P. Bouvet, and G. Marone Endogenous Superallergen Protein Fv Induces IL-4 Secretion from Human Fc{epsilon}RI+ Cells Through Interaction with the VH3 Region of IgE J. Immunol., November 15, 1998; 161(10): 5647 - 5655. [Abstract] [Full Text] [PDF]

				H. Yamamoto, J. B. Sedgwick, and W. W. Busse Differential Regulation of Eosinophil Adhesion and Transmigration by Pulmonary Microvascular Endothelial Cells J. Immunol., July 15, 1998; 161(2): 971 - 977. [Abstract] [Full Text] [PDF]

				H.-U. Simon, S. Yousefi, B. Dibbert, H. Hebestreit, M. Weber, D. R. Branch, K. Blaser, F. Levi-Schaffer, and G. P. Anderson Role for Tyrosine Phosphorylation and Lyn Tyrosine Kinase in Fas Receptor-Mediated Apoptosis in Eosinophils Blood, July 15, 1998; 92(2): 547 - 557. [Abstract] [Full Text] [PDF]

				M. Grewe, W. Czech, A. Morita, T. Werfel, M. Klammer, A. Kapp, T. Ruzicka, E. Schopf, and J. Krutmann Human Eosinophils Produce Biologically Active IL-12: Implications for Control of T Cell Responses J. Immunol., July 1, 1998; 161(1): 415 - 420. [Abstract] [Full Text] [PDF]

				M. Kobayashi, H. Kobayashi, R. B. Pollard, and F. Suzuki A Pathogenic Role of Th2 Cells and Their Cytokine Products on the Pulmonary Metastasis of Murine B16 Melanoma J. Immunol., June 15, 1998; 160(12): 5869 - 5873. [Abstract] [Full Text] [PDF]

				M.-J. Sanz, L. Marinova-Mutafchieva, P. Green, R. R. Lobb, M. Feldmann, and S. Nourshargh IL-4-Induced Eosinophil Accumulation in Rat Skin Is Dependent on Endogenous TNF-{alpha} and {alpha}4 Integrin/VCAM-1 Adhesion Pathways J. Immunol., June 1, 1998; 160(11): 5637 - 5645. [Abstract] [Full Text] [PDF]

				K. Blease, J. Seybold, I. M. Adcock, P. G. Hellewell, and A. Burke-Gaffney Interleukin-4 and Lipopolysaccharide Synergize to Induce Vascular Cell Adhesion Molecule-1 Expression in Human Lung Microvascular Endothelial Cells Am. J. Respir. Cell Mol. Biol., May 1, 1998; 18(5): 620 - 630. [Abstract] [Full Text]

				V. DE ROSE, A. OLIVA, B. MESSORE, B. GROSSO, C. MOLLAR, and E. POZZI Circulating Adhesion Molecules in Cystic Fibrosis Am. J. Respir. Crit. Care Med., April 1, 1998; 157(4): 1234 - 1239. [Abstract] [Full Text] [PDF]

				M.-J. Sanz, P. D. Ponath, C. R. Mackay, W. Newman, M. Miyasaka, T. Tamatani, B. F. Flanagan, R. R. Lobb, T. J. Williams, S. Nourshargh, et al. Human Eotaxin Induces {alpha}4 and {beta}2 Integrin-Dependent Eosinophil Accumulation in Rat Skin In Vivo: Delayed Generation of Eotaxin in Response to IL-4 J. Immunol., April 1, 1998; 160(7): 3569 - 3576. [Abstract] [Full Text] [PDF]

				D. Kuperman, B. Schofield, M. Wills-Karp, and M. J. Grusby Signal Transducer and Activator of Transcription Factor 6 (Stat6)-deficient Mice Are Protected from Antigen-induced Airway Hyperresponsiveness and Mucus Production J. Exp. Med., March 16, 1998; 187(6): 939 - 948. [Abstract] [Full Text] [PDF]

				R. H. DeKruyff, Y. Fang, and D. T. Umetsu Corticosteroids Enhance the Capacity of Macrophages to Induce Th2 Cytokine Synthesis in CD4+ Lymphocytes by Inhibiting IL-12 Production J. Immunol., March 1, 1998; 160(5): 2231 - 2237. [Abstract] [Full Text] [PDF]

				A. C. Redrup, B. P. Howard, D. W. MacGlashan Jr., A. Kagey-Sobotka, L. M. Lichtenstein, and J. T. Schroeder Differential Regulation of IL-4 and IL-13 Secretion by Human Basophils: Their Relationship to Histamine Release in Mixed Leukocyte Cultures J. Immunol., February 15, 1998; 160(4): 1957 - 1964. [Abstract] [Full Text] [PDF]

				K. A. Pacheco, M. Tarkowski, J. Klemm, and L. J. Rosenwasser CD49d Expression and Function on Allergen-stimulated T Cells from Blood and Airway Am. J. Respir. Cell Mol. Biol., February 1, 1998; 18(2): 286 - 293. [Abstract] [Full Text]

				X.-M. Li, B. H. Schofield, Q.-F. Wang, K.-H. Kim, and S.-K. Huang Induction of Pulmonary Allergic Responses by Antigen-Specific Th2 Cells J. Immunol., February 1, 1998; 160(3): 1378 - 1384. [Abstract] [Full Text] [PDF]

				W. A. Sewell, L. L. Scurr, H. Orphanides, S. Kinder, and R. I. Ludowyke Induction of Interleukin-4 and Interleukin-5 Expression in Mast Cells Is Inhibited by Glucocorticoids Clin. Vaccine Immunol., January 1, 1998; 5(1): 18 - 23. [Abstract] [Full Text]

				M.-J. Sanz, A. Hartnell, P. Chisholm, C. Williams, D. Davies, V. B. Weg, M. Feldmann, M. A. Bolanowski, R. R. Lobb, and S. Nourshargh Tumor Necrosis Factor alpha -Induced Eosinophil Accumulation in Rat Skin Is Dependent on alpha 4 Integrin/Vascular Cell Adhesion Molecule-1 Adhesion Pathways Blood, November 15, 1997; 90(10): 4144 - 4152. [Abstract] [Full Text] [PDF]

				J. Atsuta, S. A. Sterbinsky, J. Plitt, L. M. Schwiebert, B. S. Bochner, and R. P. Schleimer Phenotyping and Cytokine Regulation of the BEAS-2B Human Bronchial Epithelial Cell: Demonstration of Inducible Expression of the Adhesion Molecules VCAM-1 and ICAM-1 Am. J. Respir. Cell Mol. Biol., November 1, 1997; 17(5): 571 - 582. [Abstract] [Full Text]

				C. G. Garlisi, A. Falcone, J. A. Hey, T. M. Paster, X. Fernandez, C. A. Rizzo, M. Minnicozzi, H. Jones, M. Motasim Billah, R. W. Egan, et al. Airway Eosinophils, T Cells, Th2-Type Cytokine mRNA, and Hyperreactivity in Response to Aerosol Challenge of Allergic Mice with Previously Established Pulmonary Inflammation Am. J. Respir. Cell Mol. Biol., November 1, 1997; 17(5): 642 - 651. [Abstract] [Full Text]

				M. HUMBERT, C. J. CORRIGAN, P. KIMMITT, S. J. TILL, A. BARRY KAY, and S. R. DURHAM Relationship between IL-4 and IL-5 mRNA Expression and Disease Severity in Atopic Asthma Am. J. Respir. Crit. Care Med., September 1, 1997; 156(3): 704 - 708. [Abstract] [Full Text] [PDF]

				O. Ghaffar, S. Laberge, M. R. Jacobson, O. Lowhagen, S. Rak, S. R. Durham, and Q. Hamid IL-13 mRNA and Immunoreactivity in Allergen-induced Rhinitis: Comparison with IL-4 Expression and Modulation by Topical Glucocorticoid Therapy Am. J. Respir. Cell Mol. Biol., July 1, 1997; 17(1): 17 - 24. [Abstract] [Full Text]

				V. Gemou-Engesaeth, A. Bush, A. B. Kay, Q. Hamid, and C. J. Corrigan Inhaled Glucocorticoid Therapy of Childhood Asthma Is Associated With Reduced Peripheral Blood T Cell Activation and `Th2-Type' Cytokine mRNA Expression Pediatrics, May 1, 1997; 99(5): 695 - 703. [Abstract] [Full Text] [PDF]

				S. M. Lugli, N. Feng, M. H. Heim, M. Adam, B. Schnyder, H. Etter, M. Yamage, H.-P. Eugster, R. A. Lutz, G. Zurawski, et al. Tumor Necrosis Factor alpha Enhances the Expression of the Interleukin (IL)-4 Receptor alpha -Chain on Endothelial Cells Increasing IL-4 or IL-13-induced Stat6 Activation J. Biol. Chem., February 28, 1997; 272(9): 5487 - 5494. [Abstract] [Full Text] [PDF]

				C. J. Clark, F. Boswell, I. A. Greer, and F. Lyall Treatment of Endothelial Cells With Serum from Women with Preeclampsia: Effect on Neutrophil Adhesion Reproductive Sciences, January 1, 1997; 4(1): 27 - 33. [Abstract] [PDF]

				C. Feliciani, A.K. Gupta, and D.N. Saucier Keratinocytes and Cytokine/Growth Factors Critical Reviews in Oral Biology & Medicine, January 1, 1996; 7(4): 300 - 318. [Abstract] [Full Text] [PDF]