| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 147, Issue 9 3047-3052, Copyright © 1991 by American Association of Immunologists
ARTICLES |
A Salmeron, F Sanchez-Madrid, MA Ursa, M Fresno and B Alarcon
Centro de Biologia Molecular CSIC, Universidad Autonoma de Madrid, Spain.
We have performed immunofluorescence analysis of COS cells transfected with human CD3 genes and detergent permeabilized to define the specificity of several anti-CD3 antibodies. We have found that the mAb OKT3, WT31, UCHT1, and Leu-4 did not stain COS cells singly transfected with the CD3-epsilon chain. However, these antibodies very strongly stained COS cells doubly transfected with a combination of CD3-epsilon plus either CD3-gamma or CD3-delta. By contrast, the antibodies SP34 and APA 1/1, which were raised against isolated SDS-denatured CD3- epsilon protein, gave a strong staining of COS cells singly transfected with CD3-epsilon as well as of the double transfectans. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/epsilon and CD3- delta/epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation. These findings suggest that the most widely used mAb specific for the CD3 complex recognize conformational epitopes on CD3-epsilon, which are expressed when this chain is bound to either CD3- gamma or CD3-delta. It should also be highlighted that antibody WT31 clearly recognizes the CD3 moiety of the TCR/CD3 complex.
This article has been cited by other articles:
|
|
 |
|
  I. Stratov, A. Chung, and S. J. Kent Robust NK Cell-Mediated Human Immunodeficiency Virus (HIV)-Specific Antibody-Dependent Responses in HIV-Infected Subjects J. Virol., June 1, 2008; 82(11): 5450 - 5459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. W.A. Schamel, I. Arechaga, R. M. Risueno, H. M. van Santen, P. Cabezas, C. Risco, J. M. Valpuesta, and B. Alarcon Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response J. Exp. Med., August 15, 2005; 202(4): 493 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Delgado and B. Alarcon An orderly inactivation of intracellular retention signals controls surface expression of the T cell antigen receptor J. Exp. Med., February 22, 2005; 201(4): 555 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Arnett, S. C. Harrison, and D. C. Wiley Crystal structure of a human CD3-{epsilon}/{delta} dimer in complex with a UCHT1 single-chain antibody fragment PNAS, November 16, 2004; 101(46): 16268 - 16273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Petersen, S. Gulland, E. Bettelli, V. Kuchroo, E. Palmer, and B. T. Backstrom A chimeric T cell receptor with super-signaling properties Int. Immunol., July 1, 2004; 16(7): 889 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kjer-Nielsen, M. A. Dunstone, L. Kostenko, L. K. Ely, T. Beddoe, N. A. Mifsud, A. W. Purcell, A. G. Brooks, J. McCluskey, and J. Rossjohn Crystal structure of the human T cell receptor CD3{epsilon}{gamma} heterodimer complexed to the therapeutic mAb OKT3 PNAS, May 18, 2004; 101(20): 7675 - 7680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Blanco, P. Holliger, R. G. Vile, and L. Alvarez-Vallina Induction of Human T Lymphocyte Cytotoxicity and Inhibition of Tumor Growth by Tumor-Specific Diabody-Based Molecules Secreted from Gene-Modified Bystander Cells J. Immunol., July 15, 2003; 171(2): 1070 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-L. Law, M. Hayden-Ledbetter, S. Buckwalter, L. McNeill, H. Nguyen, P. Habecker, B. A. Thorne, R. Dua, and J. A. Ledbetter Expression and characterization of recombinant soluble human CD3 molecules: presentation of antigenic epitopes defined on the native TCR-CD3 complex Int. Immunol., April 1, 2002; 14(4): 389 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-i. Takemura, R. Asano, K. Tsumoto, S. Ebara, N. Sakurai, Y. Katayose, H. Kodama, H. Yoshida, M. Suzuki, K. Imai, et al. Construction of a diabody (small recombinant bispecific antibody) using a refolding system Protein Eng. Des. Sel., August 1, 2000; 13(8): 583 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Borroto, A. Mallabiabarrena, J. P. Albar, C. Martinez-A., and B. Alarcon Characterization of the Region Involved in CD3 Pairwise Interactions within the T Cell Receptor Complex J. Biol. Chem., May 22, 1998; 273(21): 12807 - 12816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Backstrom, B. T. Hausmann, and E. Palmer Signaling Efficiency of the T Cell Receptor Controlled by a Single Amino Acid in the beta Chain Constant Region J. Exp. Med., December 1, 1997; 186(11): 1933 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Huppa and H. L. Ploegh In Vitro Translation and Assembly of a Complete T Cell Receptor-CD3 Complex J. Exp. Med., August 4, 1997; 186(3): 393 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Bonay, M Fresno, and B Alarcon Megalomicin disrupts lysosomal functions J. Cell Sci., January 8, 1997; 110(16): 1839 - 1849. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
