The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koch, A. E.
Right arrow Articles by Strieter, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koch, A. E.
Right arrow Articles by Strieter, R. M.

The Journal of Immunology, Vol 147, Issue 7 2187-2195, Copyright © 1991 by American Association of Immunologists

ARTICLES

Synovial tissue macrophage as a source of the chemotactic cytokine IL-8

AE Koch, SL Kunkel, JC Burrows, HL Evanoff, GK Haines, RM Pope and RM Strieter
Department of Medicine, Northwestern University Medical School, Chicago, IL 60611.

Cells of the synovial microenvironment may recruit neutrophils (PMN) and lymphocytes into synovial fluid, as well as lymphocytes into the synovial tissues, of arthritic patients. We have investigated the production of the chemotactic cytokine IL-8 by using sera, synovial fluid, synovial tissue, and macrophages and fibroblasts isolated from synovial tissues from 75 arthritic patients. IL-8 levels were higher in synovial fluid from rheumatoid (RA) patients (mean +/- SE, 14.37 +/- 5.8 ng/ml), compared with synovial fluid from osteoarthritis patients (0.135 +/- 17 ng/ml) (p less than 0.05) or from patients with other arthritides (5.52 +/- 5.11 ng/ml). IL-8 from RA sera was 8.44 +/- 2.33 ng/ml, compared with nondetectable levels found in normal sera. IL-8 levels from RA sera and synovial fluid were strongly positively correlated (r = 0.96, p less than 0.05). Moreover, RA synovial fluid chemotactic activity for PMN in these fluids was inhibited 40 +/- 5% upon incubation with neutralizing polyclonal antibody to IL-8. Synovial tissue fibroblasts released only small amounts of constitutive IL-8 but could be induced to produce IL-8 by stimulation with either IL-1 beta, TNF-alpha, or LPS. In contrast, unlike normal PBMC or alveolar macrophages, macrophages isolated from RA synovial tissue constitutively expressed both IL-8 mRNA and antigenic IL-8. RA synovial macrophage IL-8 expression was not augmented by incubation with either LPS, TNF-alpha, or IL-1 beta. Immunohistochemical analysis of synovial tissue showed that a greater percentage of RA macrophages than osteoarthritis macrophages reacted with anti-IL-8. Whereas macrophages were the predominant cell for immunolocalization of IL-8, less than 5% of synovial tissue fibroblasts were positive for immunolocalized IL-8. These results suggest that macrophage-derived IL-8 may play an important role in the recruitment of PMN in synovial inflammation associated with RA.


This article has been cited by other articles:


Home page
 Ann Rheum DisHome page
S Garcia, A Bodano, J L Pablos, J J Gomez-Reino, and C Conde
Poly(ADP-ribose) polymerase inhibition reduces tumor necrosis factor-induced inflammatory response in rheumatoid synovial fibroblasts
Ann Rheum Dis, May 1, 2008; 67(5): 631 - 637.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. M. Cunha, W. A. Verri Jr., I. R. Schivo, M. H. Napimoga, C. A. Parada, S. Poole, M. M. Teixeira, S. H. Ferreira, and F. Q. Cunha
Crucial role of neutrophils in the development of mechanical inflammatory hypernociception
J. Leukoc. Biol., April 1, 2008; 83(4): 824 - 832.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
T. Haupl, M. Yahyawi, C. Lubke, J. Ringe, T. Rohrlach, G. R. Burmester, M. Sittinger, and C. Kaps
Gene Expression Profiling of Rheumatoid Arthritis Synovial Cells Treated with Antirheumatic Drugs
J Biomol Screen, April 1, 2007; 12(3): 328 - 340.
[Abstract] [PDF]

Home page
 J. Leukoc. Biol.Home page
H. Kelchtermans, S. Struyf, B. De Klerck, T. Mitera, M. Alen, L. Geboes, M. Van Balen, C. Dillen, W. Put, C. Gysemans, et al.
Protective role of IFN-{gamma} in collagen-induced arthritis conferred by inhibition of mycobacteria-induced granulocyte chemotactic protein-2 production
J. Leukoc. Biol., April 1, 2007; 81(4): 1044 - 1053.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Liu, P. Eksarko, V. Temkin, G. K. Haines III, H. Perlman, A. E. Koch, B. Thimmapaya, and R. M. Pope
Mcl-1 Is Essential for the Survival of Synovial Fibroblasts in Rheumatoid Arthritis
J. Immunol., December 15, 2005; 175(12): 8337 - 8345.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
T R D J Radstake, R van der Voort, M ten Brummelhuis, M de Waal Malefijt, M Looman, C G Figdor, W B van den Berg, P Barrera, and G J Adema
Increased expression of CCL18, CCL19, and CCL17 by dendritic cells from patients with rheumatoid arthritis, and regulation by Fc gamma receptors
Ann Rheum Dis, March 1, 2005; 64(3): 359 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
C. S. Bonnet and D. A. Walsh
Osteoarthritis, angiogenesis and inflammation
Rheumatology, January 1, 2005; 44(1): 7 - 16.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
J J Haringman, J Ludikhuize, and P P Tak
Chemokines in joint disease: the key to inflammation?
Ann Rheum Dis, October 1, 2004; 63(10): 1186 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Pierer, J. Rethage, R. Seibl, R. Lauener, F. Brentano, U. Wagner, H. Hantzschel, B. A. Michel, R. E. Gay, S. Gay, et al.
Chemokine Secretion of Rheumatoid Arthritis Synovial Fibroblasts Stimulated by Toll-Like Receptor 2 Ligands
J. Immunol., January 15, 2004; 172(2): 1256 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
S. Rosengren, G. S. Firestein, and D. L. Boyle
Measurement of Inflammatory Biomarkers in Synovial Tissue Extracts by Enzyme-Linked Immunosorbent Assay
Clin. Vaccine Immunol., November 1, 2003; 10(6): 1002 - 1010.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Kocyigit, S. Gur, M. S. Gurel, V. Bulut, and M. Ulukanligil
Antimonial Therapy Induces Circulating Proinflammatory Cytokines in Patients with Cutaneous Leishmaniasis
Infect. Immun., December 1, 2002; 70(12): 6589 - 6591.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. L. Podolin, B. J. Bolognese, J. J. Foley, D. B. Schmidt, P. T. Buckley, K. L. Widdowson, Q. Jin, J. R. White, J. M. Lee, R. B. Goodman, et al.
A Potent and Selective Nonpeptide Antagonist of CXCR2 Inhibits Acute and Chronic Models of Arthritis in the Rabbit
J. Immunol., December 1, 2002; 169(11): 6435 - 6444.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Garcia-Ramallo, T. Marques, N. Prats, J. Beleta, S. L. Kunkel, and N. Godessart
Resident Cell Chemokine Expression Serves as the Major Mechanism for Leukocyte Recruitment During Local Inflammation
J. Immunol., December 1, 2002; 169(11): 6467 - 6473.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Keane, S. C. Donnelly, J. A. Belperio, R. B. Goodman, M. Dy, M. D. Burdick, M. C. Fishbein, and R. M. Strieter
Imbalance in the Expression of CXC Chemokines Correlates with Bronchoalveolar Lavage Fluid Angiogenic Activity and Procollagen Levels in Acute Respiratory Distress Syndrome
J. Immunol., December 1, 2002; 169(11): 6515 - 6521.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
T. Fujii, T. Nojima, H. Yasuoka, S. Satoh, K. Nakamura, M. Kuwana, A. Suwa, M. Hirakata, and T. Mimori
Cytokine and immunogenetic profiles in Japanese patients with adult Still's disease. Association with chronic articular disease
Rheumatology, December 1, 2001; 40(12): 1398 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Chabaud, G. Page, and P. Miossec
Enhancing Effect of IL-1, IL-17, and TNF-{alpha} on Macrophage Inflammatory Protein-3{alpha} Production in Rheumatoid Arthritis: Regulation by Soluble Receptors and Th2 Cytokines
J. Immunol., November 15, 2001; 167(10): 6015 - 6020.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. V. Volin, J. M. Woods, M. A. Amin, M. A. Connors, L. A. Harlow, and A. E. Koch
Fractalkine: A Novel Angiogenic Chemokine in Rheumatoid Arthritis
Am. J. Pathol., October 1, 2001; 159(4): 1521 - 1530.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
E. S. White, S. R. B. Strom, N. L. Wys, and D. A. Arenberg
Non-Small Cell Lung Cancer Cells Induce Monocytes to Increase Expression of Angiogenic Activity
J. Immunol., June 15, 2001; 166(12): 7549 - 7555.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Dreier, S. Wallace, S. Fuchs, P. Bruckner, and S. Grassel
Paracrine interactions of chondrocytes and macrophages in cartilage degradation: articular chondrocytes provide factors that activate macrophage-derived pro-gelatinase B (pro-MMP-9)
J. Cell Sci., January 11, 2001; 114(21): 3813 - 3822.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Shi, K. Hayashida, M. Kaneko, J. Hashimoto, T. Tomita, P. E. Lipsky, H. Yoshikawa, and T. Ochi
Lymphoid Chemokine B Cell-Attracting Chemokine-1 (CXCL13) Is Expressed in Germinal Center of Ectopic Lymphoid Follicles Within the Synovium of Chronic Arthritis Patients
J. Immunol., January 1, 2001; 166(1): 650 - 655.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Georganas, H. Liu, H. Perlman, A. Hoffmann, B. Thimmapaya, and R. M. Pope
Regulation of IL-6 and IL-8 Expression in Rheumatoid Arthritis Synovial Fibroblasts: the Dominant Role for NF-{kappa}B But Not C/EBP{beta} or c-Jun
J. Immunol., December 15, 2000; 165(12): 7199 - 7206.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. L. van Golen, Z.-F. Wu, X. T. Qiao, L. W. Bao, and S. D. Merajver
RhoC GTPase, a Novel Transforming Oncogene for Human Mammary Epithelial Cells That Partially Recapitulates the Inflammatory Breast Cancer Phenotype
Cancer Res., October 1, 2000; 60(20): 5832 - 5838.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
J. M. Woods, K. J. Katschke Jr., M. Tokuhira, H. Kurata, K.-I. Arai, P. L. Campbell, and A. E. Koch
Reduction of Inflammatory Cytokines and Prostaglandin E2 by IL-13 Gene Therapy in Rheumatoid Arthritis Synovium
J. Immunol., September 1, 2000; 165(5): 2755 - 2763.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
B. Bresnihan, P. P. Tak, P. Emery, L. Klareskog, and F. Breedveld
Synovial biopsy in arthritis research: five years of concerted European collaboration
Ann Rheum Dis, July 1, 2000; 59(7): 506 - 511.
[Full Text]

Home page
 J. Immunol.Home page
M. Triggiani, F. Granata, A. Oriente, V. De Marino, M. Gentile, C. Calabrese, C. Palumbo, and G. Marone
Secretory Phospholipases A2 Induce {beta}-Glucuronidase Release and IL-6 Production from Human Lung Macrophages
J. Immunol., May 1, 2000; 164(9): 4908 - 4915.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Keane, J. A. Belperio, D. A. Arenberg, M. D. Burdick, Z. J. Xu, Y. Y. Xue, and R. M. Strieter
IFN-{gamma}-Inducible Protein-10 Attenuates Bleomycin-Induced Pulmonary Fibrosis Via Inhibition of Angiogenesis
J. Immunol., November 15, 1999; 163(10): 5686 - 5692.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
R. J T Rodenburg, F. H J van den Hoogen, P. Barrera, W. J van Venrooij, and L. B A van de Putte
Superinduction of interleukin 8 mRNA in activated monocyte derived macrophages from rheumatoid arthritis patients
Ann Rheum Dis, October 1, 1999; 58(10): 648 - 652.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
M. P. Keane, J. A. Belperio, T. A. Moore, B. B. Moore, D. A. Arenberg, R. E. Smith, M. D. Burdick, S. L. Kunkel, and R. M. Strieter
Neutralization of the CXC Chemokine, Macrophage Inflammatory Protein-2, Attenuates Bleomycin-Induced Pulmonary Fibrosis
J. Immunol., May 1, 1999; 162(9): 5511 - 5518.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
Y. Hatano, T. Kasama, H. Iwabuchi, R. Hanaoka, H. T Takeuchi, L. Jing, Y. Mori, K. Kobayashi, M. Negishi, H. Ide, et al.
Macrophage inflammatory protein 1 alpha expression by synovial fluid neutrophils in rheumatoid arthritis
Ann Rheum Dis, May 1, 1999; 58(5): 297 - 302.
[Abstract] [Full Text]

Home page
 BloodHome page
P. P. McDonald, M. P. Russo, S. Ferrini, and M. A. Cassatella
Interleukin-15 (IL-15) Induces NF-kappa B Activation and IL-8 Production in Human Neutrophils
Blood, December 15, 1998; 92(12): 4828 - 4835.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Parks, R. M. Strieter, N. W. Lukacs, J. Gauldie, M. Hitt, F. L. Graham, and S. L. Kunkel
Transient Gene Transfer of IL-12 Regulates Chemokine Expression and Disease Severity in Experimental Arthritis
J. Immunol., May 1, 1998; 160(9): 4615 - 4619.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. Koch, P. Polverini, S. Kunkel, L. Harlow, L. DiPietro, V. Elner, S. Elner, and R. Strieter
Interleukin-8 as a macrophage-derived mediator of angiogenesis
Science, December 11, 1992; 258(5089): 1798 - 1801.
[Abstract] [PDF]

Home page
 ScienceHome page
T Kishimoto, S Akira, and T Taga
Interleukin-6 and its receptor: a paradigm for cytokines
Science, October 23, 1992; 258(5082): 593 - 597.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.