The Journal of Immunology, Vol 147, Issue 7 2107-2115, Copyright © 1991 by American Association of Immunologists

ARTICLES

Canine neutrophil margination mediated by lectin adhesion molecule-1 in vitro

O Abbassi, CL Lane, S Krater, TK Kishimoto, DC Anderson, LV McIntire and CW Smith
Biomedical Engineering Laboratory, Rice University, Houston, TX 77251.

The contributions of the canine neutrophil lectin adhesion molecule-1 (LECAM-1) (canine homologue of the murine MEL-14 Ag) in neutrophil- endothelial cell adhesion and transendothelial migration were studied using anti-LECAM-1 mAb, CL2/6, and SL1 under static conditions and at wall shear stresses of up to 1.85 dynes/cm2 (dpc). Both mAb were found to inhibit attachment of neutrophils to cytokine-stimulated canine jugular vein endothelium. The inhibitory effects of the anti-LECAM-1 mAb were more evident at a wall shear stress of 1.85 dpc (greater than 50%) than at 0.23 dpc or under static conditions (approximately 30%). In contrast the anti-CD18 mAb, R15.7, exhibited higher inhibitory ability at the lower shear stress and under static conditions with marginal inhibition of adhesion at 1.85 dpc. Anti-LECAM-1 and anti-CD18 mAb showed additive inhibitory effects at the lower wall shear stress and under static conditions. Chemotactic stimulation of the neutrophils caused rapid down-regulation of LECAM-1 from the neutrophil surface and reduced adhesion by 60% at a wall shear stress of 1.85 dpc. This inhibition was not additive to anti-LECAM-1 mAb. Pretreatment with CL2/6 or SL1 did not affect trans-endothelial migration of adherent neutrophils under any experimental conditions tested. Anti-CD18 mAb, however, blocked transendothelial migration by 98% and 56% under static condition and at a wall shear stress of 0.23 dpc, respectively. The results in this report indicate that canine LECAM-1 is involved in the initial adhesion of unstimulated neutrophils to cytokine-stimulated endothelial cells under flow, but in contrast to CD18-integrins, plays no role in the transendothelial migration.

This article has been cited by other articles:

				P. K. Gopalan, A. R. Burns, S. I. Simon, S. Sparks, L. V. McIntire, and C. W. Smith Preferential sites for stationary adhesion of neutrophils to cytokine-stimulated HUVEC under flow conditions J. Leukoc. Biol., July 1, 2000; 68(1): 47 - 57. [Abstract] [Full Text]

				A. Nandi, P. Estess, and M. H. Siegelman Hyaluronan Anchoring and Regulation on the Surface of Vascular Endothelial Cells Is Mediated through the Functionally Active Form of CD44 J. Biol. Chem., May 12, 2000; 275(20): 14939 - 14948. [Abstract] [Full Text] [PDF]

				C. M. Doerschuk, J. P. Mizgerd, H. Kubo, L. Qin, and T. Kumasaka Adhesion Molecules and Cellular Biomechanical Changes in Acute Lung Injury: Giles F. Filley Lecture Chest, July 1, 1999; 116(2007): 37S - 43S. [Full Text]

				H. KUBO, N. A. DOYLE, L. GRAHAM, S. D. BHAGWAN, W. M. QUINLAN, and C. M. DOERSCHUK L- and P-Selectin and CD11/CD18 in Intracapillary Neutrophil Sequestration in Rabbit Lungs Am. J. Respir. Crit. Care Med., January 1, 1999; 159(1): 267 - 274. [Abstract] [Full Text]

				K. Kusterer, J. Bojunga, M. Enghofer, E. Heidenthal, K. H. Usadel, H. Kolb, and S. Martin Soluble ICAM-1 reduces leukocyte adhesion to vascular endothelium in ischemia-reperfusion injury in mice Am J Physiol Gastrointest Liver Physiol, August 1, 1998; 275(2): G377 - G380. [Abstract] [Full Text] [PDF]

				M. M. Mariscalco, M. H. Tcharmtchi, and C. W. Smith P-Selectin Support of Neonatal Neutrophil Adherence Under Flow: Contribution of L-Selectin, LFA-1, and Ligand(s) for P-Selectin Blood, June 15, 1998; 91(12): 4776 - 4785. [Abstract] [Full Text] [PDF]

				A Tozeren, H. Kleinman, S Wu, A. Mercurio, and S. Byers Integrin alpha 6 beta 4 mediates dynamic interactions with laminin J. Cell Sci., January 11, 1994; 107(11): 3153 - 3163. [Abstract] [PDF]

				M. M. Mariscalco, W. Vergara, J. Mei, E. O'B. Smith, and C. W. Smith Mechanisms of decreased leukocyte localization in the developing host Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H636 - H644. [Abstract] [Full Text] [PDF]