The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sanz, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sanz, I.

The Journal of Immunology, Vol 147, Issue 5 1720-1729, Copyright © 1991 by American Association of Immunologists

ARTICLES

Multiple mechanisms participate in the generation of diversity of human H chain CDR3 regions

I Sanz
Department of Medicine, University of Texas Health Science Center, San Antonio 78284-7874.

The area of highest variability in the antibody-combining site is the third complementarity determining region or CDR3. Based on our preliminary observation of the tremendous variability of this region in the human system we have studied the potential of CDR3 regions for generating diversity in the human B cell repertoire. To this end we generated CDR3-specific cDNA libraries from tissues collected at several stages of human development. Detailed computer analysis of more than 500 sequences reveals that human CDR3 region have the potential to generate more than 10(14) different peptides. The mechanisms responsible for this diversity include rearrangement by inversion, D-D fusion, gene conversion and the frequent utilization of the recently described DIR genes. The specific recombination mechanisms which may explain aberrant rearrangements as well as differences between fetal and adult repertoires are discussed in detail.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. M. Souto-Carneiro, R. Fritsch, N. Sepulveda, M. J. Lagareiro, N. Morgado, N. S. Longo, and P. E. Lipsky
The NF-{kappa}B Canonical Pathway Is Involved in the Control of the Exonucleolytic Processing of Coding Ends during V(D)J Recombination
J. Immunol., January 15, 2008; 180(2): 1040 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Zemlin, G. Hoersch, C. Zemlin, A. Pohl-Schickinger, M. Hummel, C. Berek, R. F. Maier, and K. Bauer
The Postnatal Maturation of the Immunoglobulin Heavy Chain IgG Repertoire in Human Preterm Neonates Is Slower than in Term Neonates
J. Immunol., January 15, 2007; 178(2): 1180 - 1188.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
E.-M. Krinner, J. Hepp, P. Hoffmann, S. Bruckmaier, L. Petersen, S. Petsch, L. Parr, I. Schuster, S. Mangold, G. Lorenczewski, et al.
A highly stable polyethylene glycol-conjugated human single-chain antibody neutralizing granulocyte-macrophage colony stimulating factor at low nanomolar concentration
Protein Eng. Des. Sel., October 1, 2006; 19(10): 461 - 470.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. C. Watson, C. S. Moffatt-Blue, R. Z. McDonald, E. Kompfner, D. Ait-Azzouzene, D. Nemazee, A. N. Theofilopoulos, D. H. Kono, and A. J. Feeney
Paucity of V-D-D-J Rearrangements and VH Replacement Events in Lupus Prone and Nonautoimmune TdT-/- and TdT+/+ Mice
J. Immunol., July 15, 2006; 177(2): 1120 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. M. Souto-Carneiro, G. P. Sims, H. Girschik, J. Lee, and P. E. Lipsky
Developmental Changes in the Human Heavy Chain CDR3
J. Immunol., December 1, 2005; 175(11): 7425 - 7436.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Q. Lou, R. J. Kelleher Jr, A. Sette, J. Loyall, S. Southwood, R. B. Bankert, and S. H. Bernstein
Germ line tumor-associated immunoglobulin VH region peptides provoke a tumor-specific immune response without altering the response potential of normal B cells
Blood, August 1, 2004; 104(3): 752 - 759.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Li, M. Rue, J. Zhou, H. Wang, M. A. Goldwasser, D. Neuberg, V. Dalton, D. Zuckerman, C. Lyons, L. B. Silverman, et al.
Utilization of Ig heavy chain variable, diversity, and joining gene segments in children with B-lineage acute lymphoblastic leukemia: implications for the mechanisms of VDJ recombination and for pathogenesis
Blood, June 15, 2004; 103(12): 4602 - 4609.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. Collins, M. Ikutani, D. Puiu, G. A. Buck, A. Nadkarni, and B. Gaeta
Partitioning of Rearranged Ig Genes by Mutation Analysis Demonstrates D-D Fusion and V Gene Replacement in the Expressed Human Repertoire
J. Immunol., January 1, 2004; 172(1): 340 - 348.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. L. Wiemels, B. C. Leonard, Y. Wang, M. R. Segal, S. P. Hunger, M. T. Smith, V. Crouse, X. Ma, P. A. Buffler, and S. R. Pine
Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia
PNAS, November 12, 2002; 99(23): 15101 - 15106.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Bauer, M. Zemlin, M. Hummel, S. Pfeiffer, J. Karstaedt, G. Steinhauser, X. Xiao, H. Versmold, and C. Berek
Diversification of Ig Heavy Chain Genes in Human Preterm Neonates Prematurely Exposed to Environmental Antigens
J. Immunol., August 1, 2002; 169(3): 1349 - 1356.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. R. Edwards, W. Brouwer, C. H. Y. Choi, J. Ruhno, R. L. Ward, and A. M. Collins
Analysis of IgE Antibodies from a Patient with Atopic Dermatitis: Biased V Gene Usage and Evidence for Polyreactive IgE Heavy Chain Complementarity-Determining Region 3
J. Immunol., June 15, 2002; 168(12): 6305 - 6313.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Kompfner, P. Oliveira, A. Montalbano, and A. J. Feeney
Unusual Germline DSP2 Gene Accounts for All Apparent V-D-D-J Rearrangements in Newborn, But Not Adult, MRL Mice
J. Immunol., December 15, 2001; 167(12): 6933 - 6938.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. W. Schroeder Jr, L. Zhang, and J. B. Philips III
Slow, programmed maturation of the immunoglobulin HCDR3 repertoire during the third trimester of fetal life
Blood, November 1, 2001; 98(9): 2745 - 2751.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
E. O. Saphire, P. W. H. I. Parren, R. Pantophlet, M. B. Zwick, G. M. Morris, P. M. Rudd, R. A. Dwek, R. L. Stanfield, D. R. Burton, and I. A. Wilson
Crystal Structure of a Neutralizing Human IgG Against HIV-1: A Template for Vaccine Design
Science, August 10, 2001; 293(5532): 1155 - 1159.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. A. Miklos, S. H. Swerdlow, and D. W. Bahler
Salivary gland mucosa-associated lymphoid tissue lymphoma immunoglobulin VH genes show frequent use of V1-69 with distinctive CDR3 features
Blood, June 15, 2000; 95(12): 3878 - 3884.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. E. Baranzini, M. C. Jeong, C. Butunoi, R. S. Murray, C. C. A. Bernard, and J. R. Oksenberg
B Cell Repertoire Diversity and Clonal Expansion in Multiple Sclerosis Brain Lesions
J. Immunol., November 1, 1999; 163(9): 5133 - 5144.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Shiokawa, F. Mortari, J. O. Lima, C. Nunez, F. E. Bertrand III, P. M. Kirkham, S. Zhu, A. P. Dasanayake, and H. W. Schroeder Jr.
IgM Heavy Chain Complementarity-Determining Region 3 Diversity Is Constrained by Genetic and Somatic Mechanisms Until Two Months After Birth
J. Immunol., May 15, 1999; 162(10): 6060 - 6070.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y. Sun, M. K. Park, J. Kim, B. Diamond, A. Solomon, and M. H. Nahm
Repertoire of Human Antibodies against the Polysaccharide Capsule of Streptococcus pneumoniae Serotype 6B
Infect. Immun., March 1, 1999; 67(3): 1172 - 1179.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Julien, M. Radosavljevic, N. Labouret, S. Camilleri-Broet, F. Davi, M. Raphael, T. Martin, and J.-L. Pasquali
AIDS Primary Central Nervous System Lymphoma: Molecular Analysis of the Expressed VH Genes and Possible Implications for Lymphomagenesis
J. Immunol., February 1, 1999; 162(3): 1551 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Hougs, L. Juul, H. J. Ditzel, C. Heilmann, A. Svejgaard, and T. Barington
The First Dose of a Haemophilus influenzae Type b Conjugate Vaccine Reactivates Memory B Cells: Evidence for Extensive Clonal Selection, Intraclonal Affinity Maturation, and Multiple Isotype Switches to IgA2
J. Immunol., January 1, 1999; 162(1): 224 - 237.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F.M. Raaphorst
Bone Marrow Transplantation, Fetal B-Cell Repertoire Development, and the Mechanism of Immune Reconstitution
Blood, December 15, 1998; 92(12): 4873 - 4874.
[Full Text] [PDF]

Home page
 BloodHome page
W.M. Aarts, R. Willemze, R.J. Bende, C.J.L.M. Meijer, S.T. Pals, and C.J.M. van Noesel
VH Gene Analysis of Primary Cutaneous B-Cell Lymphomas: Evidence for Ongoing Somatic Hypermutation and Isotype Switching
Blood, November 15, 1998; 92(10): 3857 - 3864.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Gokmen, F. M. Raaphorst, D. H. Boldt, and J. M. Teale
Ig Heavy Chain Third Complementarity Determining Regions (H CDR3s) After Stem Cell Transplantation Do Not Resemble the Developing Human Fetal H CDR3s in Size Distribution and Ig Gene Utilization
Blood, October 15, 1998; 92(8): 2802 - 2814.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. E. Adderson, A. R. Shikhman, K. E. Ward, and M. W. Cunningham
Molecular Analysis of Polyreactive Monoclonal Antibodies from Rheumatic Carditis: Human Anti-N-Acetylglucosamine/Anti-Myosin Antibody V Region Genes
J. Immunol., August 15, 1998; 161(4): 2020 - 2031.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Tuaillon and J. D. Capra
Use of D gene segments with irregular spacers in terminal deoxynucleotidyltransferase (TdT)+/+ and TdT-/- mice carrying a human Ig heavy chain transgenic minilocus
PNAS, February 17, 1998; 95(4): 1703 - 1708.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. W. Schettino, A. Cerutti, N. Chiorazzi, and P. Casali
Lack of Intraclonal Diversification in Ig Heavy and Light Chain V Region Genes Expressed by CD5+IgM+ Chronic Lymphocytic Leukemia B Cells: A Multiple Time Point Analysis
J. Immunol., January 15, 1998; 160(2): 820 - 830.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. S. Sahota, R. Garand, R. Bataille, A. J. Smith, and F. K. Stevenson
VH Gene Analysis of Clonally Related IgM and IgG From Human Lymphoplasmacytoid B-Cell Tumors With Chronic Lymphocytic Leukemia Features and High Serum Monoclonal IgG
Blood, January 1, 1998; 91(1): 238 - 243.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T.G. Willis, D.M. Jadayel, L.J.A. Coignet, M. Abdul-Rauf, J.G. Treleaven, D. Catovsky, and M.J.S. Dyer
Rapid Molecular Cloning of Rearrangements of the IGHJ Locus Using Long-Distance Inverse Polymerase Chain Reaction
Blood, September 15, 1997; 90(6): 2456 - 2464.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.