The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kabat, E. A.
Right arrow Articles by Wu, T. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kabat, E. A.
Right arrow Articles by Wu, T. T.

The Journal of Immunology, Vol 147, Issue 5 1709-1719, Copyright © 1991 by American Association of Immunologists

ARTICLES

Identical V region amino acid sequences and segments of sequences in antibodies of different specificities. Relative contributions of VH and VL genes, minigenes, and complementarity-determining regions to binding of antibody-combining sites

EA Kabat and TT Wu
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.

By examining a large database of amino acid sequences of antibodies of various specificities, we have found that many antibodies of distinctly different specificities assemble identical VL domains with different VH domains. In contrast, rarely is the same VH domain found in sets of antibodies of different specificities. We identified additional sets of antibodies of different specificities and identical sequences covering amino acid residues VH 1 to 94 and VL 1 to 95. In addition, there were segments of additional antibodies for which complete sequences were not available, but identities were seen in VL CDR1, VL CDR2, and VL CDR3 up to the VL-JL junction. The finding that there are many identical VL 1 to 95 segments with different VH 1 to 94 sequences, and vice versa, raises important questions as to the role of VH in influencing the conformation of VL and, conversely, the role of VL in influencing the conformation of VH. Evidence is also cited indicating that a single amino acid change may seriously disrupt site structure and in some instances abolish binding. Our findings suggest that it will be important in the future to investigate further conformational effects on antibody structure by using X-ray crystallography, nuclear magnetic resonance spectroscopy, or other methods, to obtain a better understanding of the functions and topography of antibody-combining sites.


This article has been cited by other articles:


Home page
 J BiochemHome page
K. Yoshinaga, M. Matsumoto, M. Torikai, K. Sugyo, S. Kuroki, K. Nogami, R. Matsumoto, S. Hashiguchi, Y. Ito, T. Nakashima, et al.
Ig L-chain Shuffling for Affinity Maturation of Phage Library-derived Human Anti-human MCP-1 Antibody Blocking its Chemotactic Activity
J. Biochem., May 1, 2008; 143(5): 593 - 601.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Yoshihara, S. Takiguchi, A. Kyuno, K. Tanaka, S. Kuba, S. Hashiguchi, Y. Ito, T. Hashimoto, T. Iwatsubo, S. Tsuyama, et al.
Immunoreactivity of Phage Library-derived Human Single-Chain Antibodies to Amyloid Beta Conformers In Vitro
J. Biochem., April 1, 2008; 143(4): 475 - 486.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
E. C. Peterson, M. Gunnell, Y. Che, R. L. Goforth, F. I. Carroll, R. Henry, H. Liu, and S. M. Owens
Using Hapten Design to Discover Therapeutic Monoclonal Antibodies for Treating Methamphetamine Abuse
J. Pharmacol. Exp. Ther., July 1, 2007; 322(1): 30 - 39.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Qian, L. A. Diaz, J. Ye, and S. H. Clarke
Dissecting the Anti-Desmoglein Autoreactive B Cell Repertoire in Pemphigus Vulgaris Patients
J. Immunol., May 1, 2007; 178(9): 5982 - 5990.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
E.-M. Krinner, J. Hepp, P. Hoffmann, S. Bruckmaier, L. Petersen, S. Petsch, L. Parr, I. Schuster, S. Mangold, G. Lorenczewski, et al.
A highly stable polyethylene glycol-conjugated human single-chain antibody neutralizing granulocyte-macrophage colony stimulating factor at low nanomolar concentration
Protein Eng. Des. Sel., October 1, 2006; 19(10): 461 - 470.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
G. Yao, W. Chen, H. Luo, Q. Jiang, Z. Xia, L. Zang, J. Zuo, X. Wei, Z. Chen, X. Shen, et al.
Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling
Int. Immunol., January 1, 2006; 18(1): 19 - 29.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
S. B. Koralov, T. I. Novobrantseva, K. Hochedlinger, R. Jaenisch, and K. Rajewsky
Direct in vivo VH to JH rearrangement violating the 12/23 rule
J. Exp. Med., February 7, 2005; 201(3): 341 - 348.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
T. Dong, G. Stewart-Jones, N. Chen, P. Easterbrook, X. Xu, L. Papagno, V. Appay, M. Weekes, C. Conlon, C. Spina, et al.
HIV-specific Cytotoxic T Cells from Long-Term Survivors Select a Unique T Cell Receptor
J. Exp. Med., December 20, 2004; 200(12): 1547 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Weekes, M. R. Wills, J. G. P. Sissons, and A. J. Carmichael
Long-Term Stable Expanded Human CD4+ T Cell Clones Specific for Human Cytomegalovirus Are Distributed in Both CD45RAhigh and CD45ROhigh Populations
J. Immunol., November 1, 2004; 173(9): 5843 - 5851.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
S. Hashiguchi, T. Nakashima, A. Nitani, T. Yoshihara, K. Yoshinaga, Y. Ito, Y. Maeda, and K. Sugimura
Human Fc{varepsilon}RI{alpha}-Specific Human Single-Chain Fv (scFv) Antibody with Antagonistic Activity toward IgE/Fc{varepsilon}RI{alpha}-Binding
J. Biochem., January 1, 2003; 133(1): 43 - 49.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Bauer, M. Zemlin, M. Hummel, S. Pfeiffer, J. Karstaedt, G. Steinhauser, X. Xiao, H. Versmold, and C. Berek
Diversification of Ig Heavy Chain Genes in Human Preterm Neonates Prematurely Exposed to Environmental Antigens
J. Immunol., August 1, 2002; 169(3): 1349 - 1356.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. W. Schroeder Jr, L. Zhang, and J. B. Philips III
Slow, programmed maturation of the immunoglobulin HCDR3 repertoire during the third trimester of fetal life
Blood, November 1, 2001; 98(9): 2745 - 2751.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Nakouzi, P. Valadon, J. Nosanchuk, N. Green, and A. Casadevall
Molecular Basis for Immunoglobulin M Specificity to Epitopes in Cryptococcus neoformans Polysaccharide That Elicit Protective and Nonprotective Antibodies
Infect. Immun., May 1, 2001; 69(5): 3398 - 3409.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Monestier, P. Decker, J.-P. Briand, J. L. Gabriel, and S. Muller
Molecular and Structural Properties of Three Autoimmune IgG Monoclonal Antibodies to Histone H2B
J. Biol. Chem., April 28, 2000; 275(18): 13558 - 13563.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Johnson and T. T. Wu
Kabat Database and its applications: 30 years after the first variability plot
Nucleic Acids Res., January 1, 2000; 28(1): 214 - 218.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Weekes, A. J. Carmichael, M. R. Wills, K. Mynard, and J. G. P. Sissons
Human CD28-CD8+ T Cells Contain Greatly Expanded Functional Virus-Specific Memory CTL Clones
J. Immunol., June 15, 1999; 162(12): 7569 - 7577.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Shiokawa, F. Mortari, J. O. Lima, C. Nunez, F. E. Bertrand III, P. M. Kirkham, S. Zhu, A. P. Dasanayake, and H. W. Schroeder Jr.
IgM Heavy Chain Complementarity-Determining Region 3 Diversity Is Constrained by Genetic and Somatic Mechanisms Until Two Months After Birth
J. Immunol., May 15, 1999; 162(10): 6060 - 6070.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
D. F. Lake, M. L. Salgaller, P. van der Bruggen, R. M. Bernstein, and J. J. Marchalonis
Construction and binding analysis of recombinant single-chain TCR derived from tumor-infiltrating lymphocytes and a cytotoxic T lymphocyte clone directed against MAGE-1
Int. Immunol., May 1, 1999; 11(5): 745 - 751.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. P. Weekes, M. R. Wills, K. Mynard, A. J. Carmichael, and J. G. P. Sissons
The Memory Cytotoxic T-Lymphocyte (CTL) Response to Human Cytomegalovirus Infection Contains Individual Peptide-Specific CTL Clones That Have Undergone Extensive Expansion In Vivo
J. Virol., March 1, 1999; 73(3): 2099 - 2108.
[Abstract] [Full Text]

Home page
 J. Exp. Med.Home page
H. Jacobs, Y. Fukita, G. T.J. van der Horst, J. de Boer, G. Weeda, J. Essers, N. de Wind, B. P. Engelward, L. Samson, S. Verbeek, et al.
Hypermutation of Immunoglobulin Genes in Memory B Cells of DNA Repair-deficient Mice
J. Exp. Med., June 1, 1998; 187(11): 1735 - 1743.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. G. Nickerson, M.-H. Tao, H.-T. Chen, J. Larrick, and E. A. Kabat
Human and Mouse Monoclonal Antibodies to Blood Group A Substance, Which Are Nearly Identical Immunochemically, Use Radically Different Primary Sequences
J. Biol. Chem., May 26, 1995; 270(21): 12457 - 12465.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Haynes, E. Stura, D Hilvert, and I. Wilson
Routes to catalysis: structure of a catalytic antibody and comparison with its natural counterpart
Science, February 4, 1994; 263(5147): 646 - 652.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. Desmyter, K. Decanniere, S. Muyldermans, and L. Wyns
Antigen Specificity and High Affinity Binding Provided by One Single Loop of a Camel Single-domain Antibody
J. Biol. Chem., July 6, 2001; 276(28): 26285 - 26290.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. T. Boder, K. S. Midelfort, and K. D. Wittrup
Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
PNAS, September 26, 2000; 97(20): 10701 - 10705.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.