| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 147, Issue 4 1139-1146, Copyright © 1991 by American Association of Immunologists
ARTICLES |
I Melamed, GP Downey, K Aktories and CM Roifman
Department of Pediatrics, Hospital for Sick Children, Toronto, Ontario, Canada.
The mechanisms responsible for initiating the conversion of globular to filamentous actin (assembly) after stimulation of B lymphocytes and the role of these cytoskeletal changes in cell activation are incompletely understood. We investigated the molecular basis of the signals leading to actin polymerization and concentrated on the involvement of guanosine triphosphate (GTP)-binding regulatory proteins, and protein kinase C (PKC). In addition, we related these early events to later events in B-cell activation, including cell proliferation. Cross- linking the Ag receptor with Staphylococcus aureus Cowan I (SAC) or anti-IgM antibodies, or stimulation of PKC with phorbol ester induced a time- and concentration-dependent increase in the filamentous actin content of B cells. Inhibition or depletion of PKC resulted in decreased actin assembly induced by anti-IgM, SAC, and PMA, suggesting that the signal for polymerization is generated distally to PKC activation. Pertussis toxin pretreatment inhibited the responses to anti-IgM and SAC but not PMA, and direct stimulation of permeabilized cells with GTP gamma S induced microfilament assembly, indicating the involvement of a GTP-binding protein for receptor-mediated events. Disruption of actin polymerization with botulinum C2 toxin or cytochalasin D inhibited the assembly of actin and [3H]TdR incorporation induced by all stimuli. We conclude that human B cell activation by receptor-mediated stimuli results in actin polymerization by signaling pathways coupled to GTP-binding proteins. These changes in the cytoskeleton may be involved in the transduction of messages leading to responses such as proliferation in B lymphocytes.
This article has been cited by other articles:
|
|
 |
|
  O. O. Onabajo, M. K. Seeley, A. Kale, B. Qualmann, M. Kessels, J. Han, T.-H. Tan, and W. Song Actin-Binding Protein 1 Regulates B Cell Receptor-Mediated Antigen Processing and Presentation in Response to B Cell Receptor Activation J. Immunol., May 15, 2008; 180(10): 6685 - 6695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Neron, G. Suck, X.-Z. Ma, D. Sakac, A. Roy, Y. Katsman, N. Dussault, C. Racine, and D. R. Branch B cell proliferation following CD40 stimulation results in the expression and activation of Src protein tyrosine kinase Int. Immunol., February 1, 2006; 18(2): 375 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Niiro, A. Allam, A. Stoddart, F. M. Brodsky, A. J. Marshall, and E. A. Clark The B Lymphocyte Adaptor Molecule of 32 Kilodaltons (Bam32) Regulates B Cell Antigen Receptor Internalization J. Immunol., November 1, 2004; 173(9): 5601 - 5609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. McLeod, A. J. Shum, R. L. Lee, F. Takei, and M. R. Gold The Rap GTPases Regulate Integrin-mediated Adhesion, Cell Spreading, Actin Polymerization, and Pyk2 Tyrosine Phosphorylation in B Lymphocytes J. Biol. Chem., March 26, 2004; 279(13): 12009 - 12019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iseki, C. Kubo, S.-M. Kwon, A. Yamaguchi, Y. Kataoka, N. Yoshida, K. Takatsu, and S. Takaki Increased Numbers of B-1 Cells and Enhanced Responses against TI-2 Antigen in Mice Lacking APS, an Adaptor Molecule Containing PH and SH2 Domains Mol. Cell. Biol., March 15, 2004; 24(6): 2243 - 2250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Baba, N. Fusaki, N. Shinya, A. Iwamatsu, and N. Hozumi Actin Tyrosine Dephosphorylation by the Src Homology 1-Containing Protein Tyrosine Phosphatase Is Essential for Actin Depolymerization After Membrane IgM Cross-Linking J. Immunol., April 1, 2003; 170(7): 3762 - 3768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. HASLER and M. ZOUALI B cell receptor signaling and autoimmunity FASEB J, October 1, 2001; 15(12): 2085 - 2098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Arrieumerlou, C. Randriamampita, G. Bismuth, and A. Trautmann Rac Is Involved in Early TCR Signaling J. Immunol., September 15, 2000; 165(6): 3182 - 3189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Rozdzial, C. M. Pleiman, J. C. Cambier, and T. H. Finkel3 pp56Lck Mediates TCR {zeta}-Chain Binding to the Microfilament Cytoskeleton J. Immunol., November 15, 1998; 161(10): 5491 - 5499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tsakiridis, A. Bergman, R. Somwar, C. Taha, K. Aktories, T. F. Cruz, A. Klip, and G. P. Downey Actin Filaments Facilitate Insulin Activation of the Src and Collagen Homologous/Mitogen-activated Protein Kinase Pathway Leading to DNA Synthesis and c-fos Expression J. Biol. Chem., October 23, 1998; 273(43): 28322 - 28331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lowin-Kropf, V. S. Shapiro, and A. Weiss Cytoskeletal Polarization of T Cells Is Regulated by an Immunoreceptor Tyrosine-based Activation Motif-dependent Mechanism J. Cell Biol., February 23, 1998; 140(4): 861 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N Barois, F Forquet, and J Davoust Actin microfilaments control the MHC class II antigen presentation pathway in B cells J. Cell Sci., January 7, 1998; 111(13): 1791 - 1800. [Abstract] [PDF]
|
|
|
|
|
|
S. N. Manie, A. R.P. Beck, A. Astier, SusanF. Law, T. Canty, H. Hirai, B. J. Druker, H. Avraham, N. Haghayeghi, M. Sattler, et al. Involvement of p130Cas and p105HEF1, a Novel Cas-like Docking Protein, in a Cytoskeleton-dependent Signaling Pathway Initiated by Ligation of Integrin or Antigen Receptor on Human B Cells J. Biol. Chem., February 14, 1997; 272(7): 4230 - 4236. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
