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The Journal of Immunology, Vol 147, Issue 4 1107-1114, Copyright © 1991 by American Association of Immunologists
ARTICLES |
GA Bishop
Department of Microbiology, University of Iowa, Iowa City 52242.
Cells of the mouse B cell clone, CH12.LX, receive Ag-dependent differentiative signals through their surface membrane class II molecules. The present study was performed to determine the role of class II cross-linking and cAMP in the successful delivery of these signals. Delivery of differentiative signals by anti-Ek mAb was increased by further cross-linking with a secondary anti-isotype antibody. Intact or (Fab')2, but not Fab forms of anti-Ek successfully delivered the Ag-dependent differentiative signal. Inability of monovalent Fab fragments to deliver the signal could not be attributed to an inability to adequately bind Ek molecules. The requirement for cAMP for class II-mediated signaling was also examined, because previous studies have implicated elevated cAMP levels as necessary for class II signaling. Both Ag-dependent, Ek-mediated differentiation and the Ek-mediated inhibition of Ag-independent LPS-induced differentiation were inhibited by the adenyl cyclase inhibitor 2'5'ddA, although elevation of cAMP was not in itself sufficient to deliver the differentiative signal. Inhibition of LPS-induced differentiation could be mediated by mAb binding to either Ek, Abk, or Abb on CH12.LX or an Ab-bearing transfectant, CH12.ABB1. This inhibition was abrogated by 2'5'ddA in the case of Ek or Abb, both of which deliver Ag-dependent differentiative signals to CH12.LX cells. In the case of Abk, which does not deliver such signals to CH12.LX, 2'5'ddA did not abrogate anti- Abk-mediated inhibition of the LPS response. The effects of 2'5'ddA were reversed by the cAMP analog, dibutyryl cAMP, and Ag-dependent- induced differentiation of CH12.LX or CH12.ABB1 was accompanied by an increase in cAMP levels.
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T. O. Nashar and J. R. Drake Dynamics of MHC Class II-Activating Signals in Murine Resting B Cells J. Immunol., January 15, 2006; 176(2): 827 - 838. [Abstract] [Full Text] [PDF]
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S. A. Haxhinasto and G. A. Bishop Synergistic B Cell Activation by CD40 and the B Cell Antigen Receptor: ROLE OF B LYMPHOCYTE ANTIGEN RECEPTOR-MEDIATED KINASE ACTIVATION AND TUMOR NECROSIS FACTOR RECEPTOR-ASSOCIATED FACTOR REGULATION J. Biol. Chem., January 23, 2004; 279(4): 2575 - 2582. [Abstract] [Full Text] [PDF]
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B. S. Hostager, S. A. Haxhinasto, S. L. Rowland, and G. A. Bishop Tumor Necrosis Factor Receptor-associated Factor 2 (TRAF2)-deficient B Lymphocytes Reveal Novel Roles for TRAF2 in CD40 Signaling J. Biol. Chem., November 14, 2003; 278(46): 45382 - 45390. [Abstract] [Full Text] [PDF]
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S. A. Haxhinasto and G. A. Bishop A Novel Interaction between Protein Kinase D and TNF Receptor-Associated Factor Molecules Regulates B Cell Receptor-CD40 Synergy J. Immunol., November 1, 2003; 171(9): 4655 - 4662. [Abstract] [Full Text] [PDF]
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 M. Leverkus, A. D. McLellan, M. Heldmann, A. O. Eggert, E.-B. Brocker, N. Koch, and E. Kampgen MHC class II-mediated apoptosis in dendritic cells: a role for membrane-associated and mitochondrial signaling pathways Int. Immunol., August 1, 2003; 15(8): 993 - 1006. [Abstract] [Full Text] [PDF]
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M. Baccam, S.-Y. Woo, C. Vinson, and G. A. Bishop CD40-Mediated Transcriptional Regulation of the IL-6 Gene in B Lymphocytes: Involvement of NF-{kappa}B, AP-1, and C/EBP J. Immunol., March 15, 2003; 170(6): 3099 - 3108. [Abstract] [Full Text] [PDF]
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S. A. Haxhinasto, B. S. Hostager, and G. A. Bishop Cutting Edge: Molecular Mechanisms of Synergy Between CD40 and the B Cell Antigen Receptor: Role for TNF Receptor-Associated Factor 2 in Receptor Interaction J. Immunol., August 1, 2002; 169(3): 1145 - 1149. [Abstract] [Full Text] [PDF]
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B. S. Hostager and G. A. Bishop Role of TNF Receptor-Associated Factor 2 in the Activation of IgM Secretion by CD40 and CD120b J. Immunol., April 1, 2002; 168(7): 3318 - 3322. [Abstract] [Full Text] [PDF]
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L. K. Busch and G. A. Bishop Multiple Carboxyl-Terminal Regions of the EBV Oncoprotein, Latent Membrane Protein 1, Cooperatively Regulate Signaling to B Lymphocytes Via TNF Receptor-Associated Factor (TRAF)-Dependent and TRAF-Independent Mechanisms J. Immunol., November 15, 2001; 167(10): 5805 - 5813. [Abstract] [Full Text] [PDF]
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I. M. Catlett, P. Xie, B. S. Hostager, and G. A. Bishop Signaling Through MHC Class II Molecules Blocks CD95-Induced Apoptosis J. Immunol., May 15, 2001; 166(10): 6019 - 6024. [Abstract] [Full Text] [PDF]
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K. D. Brown, B. S. Hostager, and G. A. Bishop Differential Signaling and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Degradation Mediated by CD40 and the Epstein-Barr Virus Oncoprotein Latent Membrane Protein 1 (LMP1) J. Exp. Med., April 16, 2001; 193(8): 943 - 954. [Abstract] [Full Text] [PDF]
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T. Matsuoka, H. Tabata, and S. Matsushita Monocytes Are Differentially Activated Through HLA-DR, -DQ, and -DP Molecules Via Mitogen-Activated Protein Kinases J. Immunol., February 15, 2001; 166(4): 2202 - 2208. [Abstract] [Full Text] [PDF]
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G. A. Bishop, Y. Hsing, B. S. Hostager, S. V. Jalukar, L. M. Ramirez, and M. A. Tomai Molecular Mechanisms of B Lymphocyte Activation by the Immune Response Modifier R-848 J. Immunol., November 15, 2000; 165(10): 5552 - 5557. [Abstract] [Full Text] [PDF]
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K. R. Bobbitt and L. B. Justement Regulation of MHC Class II Signal Transduction by the B Cell Coreceptors CD19 and CD22 J. Immunol., November 15, 2000; 165(10): 5588 - 5596. [Abstract] [Full Text] [PDF]
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S. V. Jalukar, B. S. Hostager, and G. A. Bishop Characterization of the Roles of TNF Receptor-Associated Factor 6 in CD40-Mediated B Lymphocyte Effector Functions J. Immunol., January 15, 2000; 164(2): 623 - 630. [Abstract] [Full Text] [PDF]
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B. S. Hostager and G. A. Bishop Cutting Edge: Contrasting Roles of TNF Receptor-Associated Factor 2 (TRAF2) and TRAF3 in CD40-Activated B Lymphocyte Differentiation J. Immunol., June 1, 1999; 162(11): 6307 - 6311. [Abstract] [Full Text] [PDF]
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L. K. Busch and G. A. Bishop The EBV Transforming Protein, Latent Membrane Protein 1, Mimics and Cooperates with CD40 Signaling in B Lymphocytes J. Immunol., March 1, 1999; 162(5): 2555 - 2561. [Abstract] [Full Text] [PDF]
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Y. Hsing and G. A. Bishop Requirement for Nuclear Factor-{kappa}B Activation by a Distinct Subset of CD40-Mediated Effector Functions in B Lymphocytes J. Immunol., March 1, 1999; 162(5): 2804 - 2811. [Abstract] [Full Text] [PDF]
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S. F. Greer, J. Lin, C. H. Clarke, and L. B. Justement Major Histocompatibility Class II-mediated Signal Transduction Is Regulated by the Protein-tyrosine Phosphatase CD45 J. Biol. Chem., May 8, 1998; 273(19): 11970 - 11979. [Abstract] [Full Text] [PDF]
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 O. Rotzschke, K. Falk, and J. L. Strominger Superactivation of an immune response triggered by oligomerized T cell epitopes PNAS, December 23, 1997; 94(26): 14642 - 14647. [Abstract] [Full Text] [PDF]
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H. Tabata, T. Matsuoka, F. Endo, Y. Nishimura, and S. Matsushita Ligation of HLA-DR Molecules on B Cells Induces Enhanced Expression of IgM Heavy Chain Genes in Association with Syk Activation J. Biol. Chem., November 3, 2000; 275(45): 34998 - 35005. [Abstract] [Full Text] [PDF]
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