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The Journal of Immunology, Vol 147, Issue 2 575-583, Copyright © 1991 by American Association of Immunologists

ARTICLES

HLA-binding regions of HIV-1 proteins. II. A systematic study of viral proteins

J Choppin, F Martinon, F Connan, M Pauchard, E Gomard and JP Levy
Institut Cochin de Genetique Moleculaire (ICGM), INSERM U152, Paris, France.

To detect HLA-binding peptides in 10 HIV-1 proteins (Rev, Tat, Vif, Vpr, Vpu, Gag p18, Gag p24, Gag p15, Env gp120 and Env gp41), the peptide binding assay (PBA) has been performed using three HLA class I molecules. Correlations have been searched between the PBA results and the peptide competitor activity in a functional test using HLA-A2- restricted CTL and target cells. A correlation between the data found in the PBA and well-defined CTL epitopes could be attempted only for the three Gag proteins. For these proteins, our results are in agreement with the known existence of epitopes reacting with human CD8+ CTL, with some exceptions. Together with the results reported with a panel of Nef peptides, these experiments showed that at least 18/20 of the already reported CTL epitopes from HIV-1 Gag, Nef, and Env proteins could be detected by the PBA, most (17/18) corresponding to strong reactivities. Perhaps more important, the regions of HIV-1 Gag p24 or Nef proteins that contain multiple associated CTL epitopes, with different HLA restrictions, were clearly identified by the reactivities in the PBA of several overlapping peptides and the major practical interest of the PBA might be the detection of such polyepitopic regions. Prediction are proposed in this report for 10 proteins, including several proteins for which CTL epitopes remain presently unknown.


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H. Tomiyama, H. Akari, A. Adachi, and M. Takiguchi
Different Effects of Nef-Mediated HLA Class I Down-Regulation on Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Cytolytic Activity and Cytokine Production
J. Virol., June 27, 2002; 76(15): 7535 - 7543.
[Abstract] [Full Text] [PDF]


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