The Journal of Immunology, Vol 147, Issue 12 4402-4407, Copyright © 1991 by American Association of Immunologists

ARTICLES

IFN-alpha induces autocrine production of IL-6 in myeloma cell lines

M Jourdan, XG Zhang, M Portier, JM Boiron, R Bataille and B Klein
INSERM U 291, Immunopathologie des Maladies Tumorales et Autoimmunes, Montpellier, France.

IL-6 is a major tumor growth factor in human multiple myeloma. Myeloma cell lines, which have the same phenotypic characteristics and Ig gene rearrangements as the original fresh myeloma cells and whose growth is strictly dependent on exogenous IL-6 similar to fresh myeloma cells, have been reproducibly established. We show here that IFN-alpha stimulated the growth of five of six of these human myeloma cell lines by inducing an autocrine production of IL-6 in myeloma cells. Indeed, IFN-alpha induced IL-6 mRNA accumulation and IL-6 production in myeloma cells and the IFN-alpha-induced growth of these cells was inhibited by anti-IL-6 mAb. Moreover, IFN-alpha made possible the rapid emergence of autonomously growing myeloma cell sublines, which produced IL-6 as an autocrine growth factor. As IFN-alpha has a potential therapeutical interest for multiple myeloma, the present study opens up new directions for studying its effects on the myeloma clone in vivo.

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