The Journal of Immunology, Vol 147, Issue 1 29-35, Copyright © 1991 by American Association of Immunologists

ARTICLES

A soluble form of the human T cell differentiation antigen CD27 is released after triggering of the TCR/CD3 complex

RQ Hintzen, R de Jong, CE Hack, M Chamuleau, EF de Vries, IJ ten Berge, J Borst and RA van Lier
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.

The human T cell Ag CD27 belongs to a recently defined family of cell surface receptors, including the nerve growth factor receptor, two distinct tumor necrosis factor receptors, and the B cell specific molecule CD40. On resting T cells, CD27 is a transmembrane homodimer with subunits of 50 to 55 kDa (p55). T cell activation via the TCR/CD3 complex causes a strong enhancement of p55 expression. Concomitantly, an alternative form of the CD27 molecule with a molecular mass of 28 to 32 kDa (p32) appears at the cell surface. With the use of ELISA, we here show that a soluble form of CD27 (sCD27) can be detected in the supernatant of T cells activated with anti-CD3 or combinations of anti- CD2 mAb. Moreover, sCD27 is found in both serum and urine from healthy donors. sCD27, purified from either culture supernatant or urine, has a molecular mass of 28 to 32 kDa and is, according to peptide mapping, structurally homologous to the p55 membrane form of CD27. Quantification of sCD27 levels may be used as a marker for T lymphocyte activation in vivo.

This article has been cited by other articles:

				D. V. Dolfi, A. C. Boesteanu, C. Petrovas, D. Xia, E. A. Butz, and P. D. Katsikis Late Signals from CD27 Prevent Fas-Dependent Apoptosis of Primary CD8+ T Cells J. Immunol., March 1, 2008; 180(5): 2912 - 2921. [Abstract] [Full Text] [PDF]

				R. J. Lebbink, M. C. W. van den Berg, T. de Ruiter, N. Raynal, J. A. G. van Roon, P. J. Lenting, B. Jin, and L. Meyaard The Soluble Leukocyte-Associated Ig-Like Receptor (LAIR)-2 Antagonizes the Collagen/LAIR-1 Inhibitory Immune Interaction J. Immunol., February 1, 2008; 180(3): 1662 - 1669. [Abstract] [Full Text] [PDF]

				J. Wischhusen, G. Jung, I. Radovanovic, C. Beier, J. P. Steinbach, A. Rimner, H. Huang, J. B. Schulz, H. Ohgaki, A. Aguzzi, et al. Identification of CD70-mediated Apoptosis of Immune Effector Cells as a Novel Immune Escape Pathway of Human Glioblastoma Cancer Res., May 1, 2002; 62(9): 2592 - 2599. [Abstract] [Full Text] [PDF]

				P. E. P. Dekkers, T. ten Hove, F. N. Lauw, H. R. Koene, P. Lumley, S. J. H. van Deventer, and T. van der Poll The Metalloproteinase Inhibitor GI5402 Inhibits Endotoxin-Induced Soluble CD27 and CD16 Release in Healthy Humans Infect. Immun., May 1, 2000; 68(5): 3036 - 3039. [Abstract] [Full Text] [PDF]

				R. Q Hintzen, D. Paty, and J. Oger Cerebrospinal fluid concentrations of soluble CD27 in HTLV-I associated myelopathy and multiple sclerosis J. Neurol. Neurosurg. Psychiatry, June 1, 1999; 66(6): 791 - 793. [Abstract] [Full Text]

				A. Al-Shamkhani, S. Mallett, M. H. Brown, W. James, and A. N. Barclay Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells J. Biol. Chem., February 21, 1997; 272(8): 5275 - 5282. [Abstract] [Full Text] [PDF]