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The Journal of Immunology, Vol 147, Issue 1 168-173, Copyright © 1991 by American Association of Immunologists

ARTICLES

Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies

T Saito, K Yasukawa, H Suzuki, K Futatsugi, T Fukunaga, C Yokomizo, Y Koishihara, H Fukui, Y Ohsugi and H Yawata
Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan.

Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One of the clones, RS13, produced IgG2a isotype that was capable of inhibiting IL-6 activity. ELISA for the quantitation of smIL- 6R was established, which could detect smIL-6R in a quantity as low as 1 ng/ml.


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