The Journal of Immunology, Vol 146, Issue 7 2341-2344, Copyright © 1991 by American Association of Immunologists

ARTICLES

Autoantibodies to the centrosome (centriole) react with determinants present in the glycolytic enzyme enolase

JB Rattner, L Martin, DM Waisman, SA Johnstone and MJ Fritzler
Department of Anatomy, University of Calgary, Alberta, Canada.

Autoantibodies to cellular Ag are found in the sera of patients with systemic rheumatic diseases. Identification and characterization of the reactive autoantigens has helped clinicians to define subsets of rheumatic diseases and has assisted biologists in defining the function within the cell of these molecules. We have studied autoantibodies from patients that react with the centrosome (centriole) region of the cell. We found by immunoblotting techniques that these antibodies react with a 48-kDa protein. Additional immunoblotting and affinity purification studies indicate that the Ag may be the glycolytic enzyme enolase.

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