The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Devens, B. H.
Right arrow Articles by Webb, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Devens, B. H.
Right arrow Articles by Webb, D. R.

The Journal of Immunology, Vol 146, Issue 5 1394-1401, Copyright © 1991 by American Association of Immunologists

ARTICLES

Involvement of two distinct regulatory T cell populations in the antigen-specific suppression of cytolytic T cell generation

BH Devens, AW Koontz, JA Kapp, CW Pierce and DR Webb
Department of Inflammation Biology and Immunology, Syntex Research, Palo Alto, CA 94303.

Alloantigen-specific, radiation-resistant T cells generated in mixed- lymphocyte cultures inhibited the generation of allospecific CTL responses in vitro. This regulatory T cell population was studied using mAb generated to Ag-specific suppressor factors that regulate the response to the synthetic terpolymer L-glutamic acid60-L-alanine30-L- tyrosine10 (GAT). Both monoclonal 984 D4.6.5 and a pool of four mAb 2441, when added in the presence of complement, eliminated alloantigen- specific inhibition of the CTL response. When separate cell cultures treated with mAb 984 or 2441 plus complement were recombined, inhibition was reestablished, suggesting that two or more populations of cells are required for active inhibition. Furthermore, neither the mAb 984 nor the mAb 2441 plus complement had any effect on any stage of CTL development. This suggests that the inhibition of the CTL response was not the result of cytolytic activity via the regulatory T cells. Experiments in which these antibodies were added without complement treatment showed that the mAb 2441 neutralized the inhibitory activity, whereas mAb 984 augmented inhibition. It is concluded from these studies that regulatory T cells originally identified in humoral immune responses also regulate cell-mediated immune responses. Suppressor epitopes are displayed on the surface of these cells that allow them to be distinguished from other T cells. These data also show the utility of the mAb 984 and 2441 raised against specific suppressor T cell products in different experimental models of immunity. These studies suggest that phenotypically distinct Ts cell populations can play a normal regulatory role in both cell-mediated and humoral immunity.


This article has been cited by other articles:


Home page
 BloodHome page
A. Noble, A. Giorgini, and J. A. Leggat
Cytokine-induced IL-10-secreting CD8 T cells represent a phenotypically distinct suppressor T-cell lineage
Blood, June 1, 2006; 107(11): 4475 - 4483.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
J. C. Zimring, S. B. Levery, B. Kniep, L. M. Kapp, M. Fuller, and J. A. Kapp
CD75s is a marker of murine CD8+ suppressor T cells
Int. Immunol., November 1, 2003; 15(11): 1389 - 1399.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.