The Journal of Immunology, Vol 146, Issue 3 936-940, Copyright © 1991 by American Association of Immunologists

ARTICLES

Expression of VHIII-associated cross-reactive idiotype on human B lymphocytes. Association with staphylococcal protein A binding and Staphylococcus aureus Cowan I stimulation

F Shokri, RA Mageed, BR Maziak and R Jefferis
Department of Immunology, Medical School, University of Birmingham, United Kingdom.

It has been demonstrated that staphylococcal protein A (SPA) has an "alternative" binding site with specificity for human Ig H chain V region of the VHIII subgroup. Because the major mitogenic component of Staphylococcus aureus Cowan I (SAC) is SPA, it is possible that SAC stimulates a subpopulation of B cells expressing Ig of the VHIII H chain subgroup. In the present study, we have investigated further the relationship between SPA binding and the expression of VHI- or VHIII- associated cross-reactive idiotype (CRI) on the surface of tonsillar B lymphocytes enriched for the expression or nonexpression of the CRI, and we examined the Ig secreted by cell lines established from these populations of B cells by EBV transformation. The VHIII CRI (D12)- enriched population yielded 21 cell lines, with 67% of them secreting SPA-reactive Ig; in contrast, only 6% (1 of 16) of VHI CRI-expressing lines secreted SPA-reactive Ig. The CRI-negative B cell population yielded 54 cell lines, of which 20% secreted SPA-reactive Ig, as might be anticipated because a majority of VHIII Ig+ B cells will be CRI-. SAC stimulation of CRI+ and CRI- populations showed preferential stimulation of the D12 population. These data support the proposal that SAC stimulation of human B cells is mediated through binding of SPA by its alternative binding site to IgV regions of the VHIII subgroup.

This article has been cited by other articles:

				E. Mikaelsson, A. H. Danesh-Manesh, A. Luppert, M. Jeddi-Tehrani, M.-R. Rezvany, R. A. Sharifian, R. Safaie, A. Roohi, A. Osterborg, F. Shokri, et al. Fibromodulin, an extracellular matrix protein: characterization of its unique gene and protein expression in B-cell chronic lymphocytic leukemia and mantle cell lymphoma Blood, June 15, 2005; 105(12): 4828 - 4835. [Abstract] [Full Text] [PDF]

				J. H. Roark, J. B. Bussel, D. B. Cines, and D. L. Siegel Genetic analysis of autoantibodies in idiopathic thrombocytopenic purpura reveals evidence of clonal expansion and somatic mutation Blood, July 30, 2002; 100(4): 1388 - 1398. [Abstract] [Full Text] [PDF]

				J. S Axford, D. H E Rees, R. A Mageed, P. Wordsworth, A. Alavi, and A. C Steere Increased IgA rheumatoid factor and VH1 associated cross reactive idiotype expression in patients with Lyme arthritis and neuroborreliosis Ann Rheum Dis, December 1, 1999; 58(12): 757 - 761. [Abstract] [Full Text]

				M. E. Massari, R. R. Rivera, J. R. Voland, M. W Quong, T. M. Breit, J. J. M. van Dongen, O. de Smit, and C. Murre Characterization of ABF-1, a Novel Basic Helix-Loop-Helix Transcription Factor Expressed in Activated B Lymphocytes Mol. Cell. Biol., June 1, 1998; 18(6): 3130 - 3139. [Abstract] [Full Text]

				L Berberian, L Goodglick, T. Kipps, and J Braun Immunoglobulin VH3 gene products: natural ligands for HIV gp120 Science, September 17, 1993; 261(5128): 1588 - 1591. [Abstract] [PDF]