The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hori, T.
Right arrow Articles by Spits, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hori, T.
Right arrow Articles by Spits, H.

The Journal of Immunology, Vol 146, Issue 12 4078-4084, Copyright © 1991 by American Association of Immunologists

ARTICLES

Identification of a novel human thymocyte subset with a phenotype of CD3- CD4+ CD8 alpha + beta-1. Possible progeny of the CD3- CD4- CD8- subset

T Hori, J Cupp, N Wrighton, F Lee and H Spits
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.

We investigated responsiveness to cytokines and differentiating potential of early human T cell precursors in vitro. Human CD3- CD4- CD8- (triple negative) thymocytes were highly purified by using magnetic bead columns and cell sorting. These cells proliferated for the first 3 to 4 days and then remained viable for up to 14 days in the presence of IL-7, IL-2 or IL-4 had only limited growth-promoting activity on these cells and could not maintain the cell viability. We followed the phenotypic change of triple negative thymocytes during culture with IL-7. After 7 to 14 days of culture with IL-7, a considerable proportion became CD4+ CD8+ (double positive). These cells were found to be CD3- CD4+ CD8 alpha+ beta- in contrast to common double positive thymocytes, which express low levels of CD3 and both alpha- and beta-chains of CD8. By using four-color immunofluorescence and multi-parameter cytofluorometric analysis, we could identify this novel subset in fresh thymocytes. These results suggest that the CD3- CD4+ CD8 alpha+ beta- subset exists physiologically in the human thymus and may represent an intermediate stage between triple negative and common double positive thymocytes.


This article has been cited by other articles:


Home page
 BloodHome page
H. Schmidlin, W. Dontje, F. Groot, S. J. Ligthart, A. D. Colantonio, M. E. Oud, E. J. Schilder-Tol, M. Spaargaren, H. Spits, C. H. Uittenbogaart, et al.
Stimulated plasmacytoid dendritic cells impair human T-cell development
Blood, December 1, 2006; 108(12): 3792 - 3800.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
L. Kobari, M. C. Giarratana, J. C. Gluckman, L. Douay, and M. Rosenzwajg
Ex Vivo Expansion Does Not Alter the Capacity of Umbilical Cord Blood CD34+ Cells to Generate Functional T Lymphocytes and Dendritic Cells
Stem Cells, September 1, 2006; 24(9): 2150 - 2157.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
A. Miyake, K. Ibuki, Y. Enose, H. Suzuki, R. Horiuchi, M. Motohara, N. Saito, T. Nakasone, M. Honda, T. Watanabe, et al.
Rapid dissemination of a pathogenic simian/human immunodeficiency virus to systemic organs and active replication in lymphoid tissues following intrarectal infection.
J. Gen. Virol., May 1, 2006; 87(Pt 5): 1311 - 1320.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. L. Joachims, J. L. Chain, S. W. Hooker, C. J. Knott-Craig, and L. F. Thompson
Human {alpha}beta and {gamma}{delta} Thymocyte Development: TCR Gene Rearrangements, Intracellular TCRbeta Expression, and {gamma}{delta} Developmental Potential--Differences between Men and Mice
J. Immunol., February 1, 2006; 176(3): 1543 - 1552.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Schotte, M.-C. Rissoan, N. Bendriss-Vermare, J.-M. Bridon, T. Duhen, K. Weijer, F. Briere, and H. Spits
The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development
Blood, February 1, 2003; 101(3): 1015 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Yahata, K. Ando, Y. Nakamura, Y. Ueyama, K. Shimamura, N. Tamaoki, S. Kato, and T. Hotta
Functional Human T Lymphocyte Development from Cord Blood CD34+ Cells in Nonobese Diabetic/Shi-scid, IL-2 Receptor {gamma} Null Mice
J. Immunol., July 1, 2002; 169(1): 204 - 209.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. R. Carrasco, C. Trigueros, A. R. Ramiro, V. G. de Yebenes, and M. L. Toribio
beta -Selection Is Associated With the Onset of CD8beta Chain Expression on CD4+CD8alpha alpha + Pre-T Cells During Human Intrathymic Development
Blood, November 15, 1999; 94(10): 3491 - 3498.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. C.M. Res, F. Couwenberg, F. A. Vyth-Dreese, and H. Spits
Expression of pTalpha mRNA in a Committed Dendritic Cell Precursor in the Human Thymus
Blood, October 15, 1999; 94(8): 2647 - 2657.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Blom, M. C.M. Verschuren, M. H.M. Heemskerk, A. Q. Bakker, E. J. van Gastel-Mol, I. L.M. Wolvers-Tettero, J. J.M. van Dongen, and H. Spits
TCR Gene Rearrangements and Expression of the Pre-T Cell Receptor Complex During Human T-Cell Differentiation
Blood, May 1, 1999; 93(9): 3033 - 3043.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. O. Muench, M. G. Roncarolo, and R. Namikawa
Phenotypic and Functional Evidence for the Expression of CD4 by Hematopoietic Stem Cells Isolated From Human Fetal Liver
Blood, February 15, 1997; 89(4): 1364 - 1375.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1991 by The American Association of Immunologists, Inc. All rights reserved.