| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 145, Issue 9 3033-3040, Copyright © 1990 by American Association of Immunologists
ARTICLES |
CA Hebert, FW Luscinskas, JM Kiely, EA Luis, WC Darbonne, GL Bennett, CC Liu, MS Obin, MA Gimbrone Jr and JB Baker
Genentech, Inc., South San Francisco, CA 94080.
We have recently shown that endothelial cell-derived IL-8 inhibits neutrophil adhesion to IL1-beta-activated human umbilical vein endothelial cell monolayers. IL-8 secreted by T lymphocytes or monocytes has been characterized as a promoter of neutrophil degranulation and chemotaxis. The IL-8 isolated from each of these cell types is a mixture of two IL-8 polypeptides, one consisting of 72 amino acids (herein called [ser-IL-8]72) and the other 77 amino acids (an N- terminal extended form herein called [ala-IL-8]77). IL-8 derived from T lymphocytes and monocytes is predominantly [ser-IL-8]72, whereas endothelial-derived IL-8 is highly enriched (greater than 80%) in [ala- IL-8]77. We address the relationship and activities of these two forms of IL-8 using recombinant proteins expressed by both mammalian cells and Escherichia coli. Thrombin was found to efficiently convert [ala-IL- 8]77 to [ser-IL-8]72. In contrast, urokinase and tissue-type plasminogen activator were unable to cleave [ala-IL-8]77, and trypsin generated multiple IL-8 cleavage fragments. In competitive binding assays using 125I[ala-IL-8]77 neutrophils exhibited a twofold preference for [ser-IL-8]72 over [ala-IL-8]77. Both forms of IL-8 inhibited neutrophil adhesion to IL-1-beta-activated HUVEC monolayers by up to 90%. However, [ser-IL-8]72 was approximately 10-fold more potent than [ala-IL-8]77 in these assays (ED50 approximately 0.3 nM for [ser-IL-8]72 vs approximately 3 nM for [ala-IL-8]77. Both forms of IL-8 promoted degranulation of cytochalasin B-treated neutrophils [[ser-IL- 8]72 (ED50 greater than 10 nM) was two- to three-fold more potent than [ala-IL-8]77], although in this regard they were less active than FMLP. Our data suggest that [ala-IL-8]77 and [ser-IL-8]72 have qualitatively similar and potentially complex biological activities, and that full activation of IL-8 requires cleavage to the [ser-IL-8]72 form. In the case of inflamed endothelial cells this activation could be mediated by thrombin generated in the procoagulant environment associated with these cells.
This article has been cited by other articles:
|
|
 |
|
  T. Loos, A. Mortier, M. Gouwy, I. Ronsse, W. Put, J.-P. Lenaerts, J. Van Damme, and P. Proost Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation Blood, October 1, 2008; 112(7): 2648 - 2656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Proost, T. Loos, A. Mortier, E. Schutyser, M. Gouwy, S. Noppen, C. Dillen, I. Ronsse, R. Conings, S. Struyf, et al. Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation J. Exp. Med., September 1, 2008; 205(9): 2085 - 2097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Skov, F. J. Beurskens, C. O. C. Zachariae, S. Reitamo, J. Teeling, D. Satijn, K. M. Knudsen, E. P. J. Boot, D. Hudson, O. Baadsgaard, et al. IL-8 as Antibody Therapeutic Target in Inflammatory Diseases: Reduction of Clinical Activity in Palmoplantar Pustulosis J. Immunol., July 1, 2008; 181(1): 669 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. H. K. Dias, L. Marshall, P. A. Lambert, I. L. C. Chapple, J. B. Matthews, and H. R. Griffiths Gingipains from Porphyromonas gingivalis Increase the Chemotactic and Respiratory Burst-Priming Properties of the 77-Amino-Acid Interleukin-8 Variant Infect. Immun., January 1, 2008; 76(1): 317 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moscova, D. J. Marsh, and R. C. Baxter Protein Chip Discovery of Secreted Proteins Regulated by the Phosphatidylinositol 3-Kinase Pathway in Ovarian Cancer Cell Lines Cancer Res., February 1, 2006; 66(3): 1376 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Berahovich, Z. Miao, Y. Wang, B. Premack, M. C. Howard, and T. J. Schall Proteolytic Activation of Alternative CCR1 Ligands in Inflammation J. Immunol., June 1, 2005; 174(11): 7341 - 7351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-Y. Choi, E.-C. Kim, H.-M. Oh, S. Kim, H.-J. Lee, E.-Y. Cho, K.-H. Yoon, E.-A Kim, W.-C. Han, S.-C. Choi, et al. Iron Chelator Triggers Inflammatory Signals in Human Intestinal Epithelial Cells: Involvement of p38 and Extracellular Signal-Regulated Kinase Signaling Pathways J. Immunol., June 1, 2004; 172(11): 7069 - 7077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mukaida Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases Am J Physiol Lung Cell Mol Physiol, April 1, 2003; 284(4): L566 - L577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Valenzuela-Fernandez, T. Planchenault, F. Baleux, I. Staropoli, K. Le-Barillec, D. Leduc, T. Delaunay, F. Lazarini, J.-L. Virelizier, M. Chignard, et al. Leukocyte Elastase Negatively Regulates Stromal Cell-derived Factor-1 (SDF-1)/CXCR4 Binding and Functions by Amino-terminal Processing of SDF-1 and CXCR4 J. Biol. Chem., May 3, 2002; 277(18): 15677 - 15689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Van den Steen, P. Proost, A. Wuyts, J. Van Damme, and G. Opdenakker Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-alpha and leaves RANTES and MCP-2 intact Blood, October 15, 2000; 96(8): 2673 - 2681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Sitrin, P. M. Pan, H. A. Harper, R. F. Todd III, D. M. Harsh, and R. A. Blackwood Clustering of Urokinase Receptors (uPAR; CD87) Induces Proinflammatory Signaling in Human Polymorphonuclear Neutrophils J. Immunol., September 15, 2000; 165(6): 3341 - 3349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Topham, H. J. Carveth, T. M. Mcintyre, S. M. Prescott, and G. A. Zimmerman Human endothelial cells regulate polymorphonuclear leukocyte degranulation FASEB J, June 1, 1998; 12(9): 733 - 746. [Abstract] [Full Text]
|
|
|
|
|
|
K. Johnson, Y. Choi, E. DeGroot, I. Samuels, A. Creasey, and L. Aarden Potential Mechanisms for a Proinflammatory Vascular Cytokine Response to Coagulation Activation J. Immunol., May 15, 1998; 160(10): 5130 - 5135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Proost, S. Struyf, M. Couvreur, J.-P. Lenaerts, R. Conings, P. Menten, P. Verhaert, A. Wuyts, and J. V. Damme Posttranslational Modifications Affect the Activity of the Human Monocyte Chemotactic Proteins MCP-1 and MCP-2: Identification of MCP-2(6-76) as a Natural Chemokine Inhibitor J. Immunol., April 15, 1998; 160(8): 4034 - 4041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Pal, A. Garzino-Demo, P. D. Markham, J. Burns, M. Brown, R. C. Gallo, and A. L. DeVico Inhibition of HIV-1 Infection by the  -Chemokine MDCScience, October 24, 1997; 278(5338): 695 - 698. [Abstract] [Full Text]
|
|
|
|
|
|
H. B. Lowman, P. H. Slagle, L. E. DeForge, C. M. Wirth, B. L. Gillece-Castro, J. H. Bourell, and W. J. Fairbrother Exchanging Interleukin-8 and Melanoma Growth-stimulating Activity Receptor Binding Specificities J. Biol. Chem., June 14, 1996; 271(24): 14344 - 14352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Williams, N. Borkakoti, G. A. Bottomley, I. Cowan, A. G. Fallowfield, P. S. Jones, S. J. Kirtland, G. J. Price, and L. Price Mutagenesis Studies of Interleukin-8 J. Biol. Chem., April 19, 1996; 271(16): 9579 - 9586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhao-hai, W. Zi-xuan, R. Horuk, J. Hesselgesser, L. Yan-chun, T. J. Hadley, and S. C. Peiper The Promiscuous Chemokine Binding Profile of the Duffy Antigen/Receptor for Chemokines Is Primarily Localized to Sequences in the Amino-terminal Domain J. Biol. Chem., November 3, 1995; 270(44): 26239 - 26245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Daly, G. J. LaRosa, S. Dolich, T. E. Maione, S. Cooper, and H. E. Broxmeyer High Activity Suppression of Myeloid Progenitor Proliferation by Chimeric Mutants of Interleukin 8 and Platelet Factor 4 J. Biol. Chem., October 6, 1995; 270(40): 23282 - 23292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Graber, A. E. I. Proudfoot, F. Talabot, A. Bernard, M. McKinnon, M. Banks, D. Fattah, R. Solari, M. C. Peitsch, and T. N. C. Wells Identification of Key Charged Residues of Human Interleukin-5 in Receptor Binding and Cellular Activation J. Biol. Chem., June 30, 1995; 270(26): 15762 - 15769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dunlevy and J. Couchman Interleukin-8 induces motile behavior and loss of focal adhesions in primary fibroblasts J. Cell Sci., January 1, 1995; 108(1): 311 - 321. [Abstract] [PDF]
|
|
|
|
 |
|
 R. F. Holcombe, B. A. Baethge, R. E. Wolf, K. W. Betzing, R. M. Stewart, V. C. Hall, and M. Fukuda Correlation of Serum Interleukin-8 and Cell Surface Lysosome-associated Membrane Protein with Clinical Disease Activity in Systemic Lupus Erythematosus Lupus, April 1, 1994; 3(2): 97 - 102. [Abstract] [PDF]
|
|
|
|
|
|
R Horuk, C. Chitnis, W. Darbonne, T. Colby, A Rybicki, T. Hadley, and L. Miller A receptor for the malarial parasite Plasmodium vivax: the erythrocyte chemokine receptor Science, August 27, 1993; 261(5125): 1182 - 1184. [Abstract] [PDF]
|
|
|
|
|
|
W. Holmes, J Lee, W. Kuang, G. Rice, and W. Wood Structure and functional expression of a human interleukin-8 receptor Science, September 13, 1991; 253(5025): 1278 - 1280. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
