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The Journal of Immunology, Vol 145, Issue 9 3011-3016, Copyright © 1990 by American Association of Immunologists
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CR Wood, AJ Dorner, GE Morris, EM Alderman, D Wilson, RM O'Hara Jr and RJ Kaufman
Genetics Institute, Inc., Cambridge, MA 02140.
The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell- synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.
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