The Journal of Immunology, Vol 145, Issue 5 1407-1414, Copyright © 1990 by American Association of Immunologists

ARTICLES

Down-regulation of IL-2 production by activation of T cells through Ly- 6A/E

EK Codias, JE Rutter, TJ Fleming and TR Malek
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.

Ly-6A/E molecules are expressed on the surface of T cells and have been shown to function in activation by the capacity of anti-Ly-6A/E mAb to induce T cell hybridomas or normal T cells to produce IL-2. Recent evidence suggests that activation through Ly-6A/E may be linked to the TCR signaling pathway. To further investigate the relationship between Ly-6- and TCR-induced T cell activation, we have examined whether an anti-Ly-6A/E mAb (D7) modulates TCR signaling in vitro. We now report that mAb D7 specifically inhibited IL-2 production by T cells also activated through TCR. Such inhibition was noted for normal T cells stimulated by soluble anti-CD3 or alloantigen and for T hybridomas stimulated by soluble anti-CD3. The ability of D7 to inhibit IL-2 production by T hybridomas was dependent on the nature of the TCR activating signal because IL-2 production was not inhibited when T hybridomas were stimulated with Ag or immobilized anti-CD3. Inhibition of IL-2 production by D7 apparently required cross-linking of the mAb because D7 F(ab')2 fragments were not effective for inhibition of IL-2 production. Similar to its ability to enhance anti-Ly-6A/E-induced activation of T and B cells, IFN-gamma enhanced the D7-induced inhibition of IL-2 production by alloantigen-activated normal T cells. These data further support the notion that Ly-6 and TCR signaling pathways are interrelated.

This article has been cited by other articles:

				C. Holmes and W. L. Stanford Concise Review: Stem Cell Antigen-1: Expression, Function, and Enigma Stem Cells, June 1, 2007; 25(6): 1339 - 1347. [Abstract] [Full Text] [PDF]

				C. Y. Ito, C. Y. J. Li, A. Bernstein, J. E. Dick, and W. L. Stanford Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice Blood, January 15, 2003; 101(2): 517 - 523. [Abstract] [Full Text] [PDF]

				S. C. Henderson, M. M. Kamdar, and A. Bamezai Ly-6A.2 Expression Regulates Antigen-Specific CD4+ T Cell Proliferation and Cytokine Production J. Immunol., January 1, 2002; 168(1): 118 - 126. [Abstract] [Full Text] [PDF]

				M. D. Marmor, M. F. Bachmann, P. S. Ohashi, T. R. Malek, and M. Julius Immobilization of glycosylphosphatidylinositol-anchored proteins inhibits T cell growth but not function Int. Immunol., September 1, 1999; 11(9): 1381 - 1393. [Abstract] [Full Text] [PDF]

				A. Kosugi, S.-i. Saitoh, S. Noda, K. Miyake, Y. Yamashita, M. Kimoto, M. Ogata, and T. Hamaoka Physical and Functional Association between Thymic Shared Antigen-1/Stem Cell Antigen-2 and the T Cell Receptor Complex J. Biol. Chem., May 15, 1998; 273(20): 12301 - 12306. [Abstract] [Full Text] [PDF]

				W. L. Stanford, S. Haque, R. Alexander, X. Liu, A. M. Latour, H. R. Snodgrass, B. H. Koller, and P. M. Flood Altered Proliferative Response by T Lymphocytes of Ly-6A (Sca-1) Null Mice J. Exp. Med., August 29, 1997; 186(5): 705 - 717. [Abstract] [Full Text] [PDF]