The JI Acurri Cytometers
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iwasaki, A.
Right arrow Articles by Nomoto, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwasaki, A.
Right arrow Articles by Nomoto, K.

The Journal of Immunology, Vol 145, Issue 1 28-35, Copyright © 1990 by American Association of Immunologists

ARTICLES

Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus

A Iwasaki, Y Yoshikai, M Sakumoto, K Himeno, H Yuuki, M Kumamoto, K Sueishi and K Nomoto
Department of Immunology, School of Medicine, Kyushu University, Fukuoka, Japan.

To elucidate the abnormality of T cell differentiation in nude mice grafted with rat fetal thymus that develop multiple-organ-localized autoimmune diseases, we examined sequential appearance of T cell subsets and expression of TCR genes in BALB/c nude mice after grafting with fetal F344 rat thymus. We observed progressive expression of TCR gamma/delta-alpha/beta genes in the lymph node (LN) cells from 8 to 12 wk after grafting. An appreciable number of CD4+ T cells but few CD8+ T cells were detected in the LN at 8 wk after grafting. CD8+ T cells increased slowly in number by 12 wk after grafting but remained at a low level in comparison with those in nude mice 12 wk after grafting with BALB/c thymus. In correlation with an increase in the number of T cells expressing TCR alpha/beta genes, alloreactivity as assessed by MLR was increased to a normal level. However, CTL activity against alloantigens remained at a low level in the LN cells at 12 wk. At this stage, organ-specific autoimmune diseases and a high level of anti-DNA autoantibodies were detected. In these mice host-reactive T cells such as V beta 3- or V beta 11-bearing T cells were virtually eliminated in the peripheral mature T cell pool, whereas T cells maturing in the fetal rat thymus significantly proliferated in response to donor-rat stimulator cells. These results suggest that the development of the autoimmune diseases may be ascribed to an impaired maturation of CD8+ T cells but not to failure in clonal elimination of host-reactive T cells in nude mice grafted with rat thymus.


This article has been cited by other articles:


Home page
 IOVSHome page
T. Hattori, M. Takeuchi, K. Ohno, M. Ogawara, T. Asatani, Y. Usui, R. Muramatsu, M. Inagaki, M. Usui, and O. Taguchi
Alpha-internexin as a Corneal Autoantigen in Spontaneous Autoimmune Keratitis Mouse Model
Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 249 - 255.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Yan, T. Devos, L. Yu, G. Xia, O. Rutgeerts, J. Goebels, C. Segers, Y. Lin, M. Vandeputte, and M. Waer
Pathogenesis of Autoimmunity After Xenogeneic Thymus Transplantation
J. Immunol., June 15, 2003; 170(12): 5936 - 5946.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Xia, J. Goebels, O. Rutgeerts, M. Vandeputte, and M. Waer
Transplantation Tolerance and Autoimmunity After Xenogeneic Thymus Transplantation
J. Immunol., February 1, 2001; 166(3): 1843 - 1854.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.