The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meek, K.
Right arrow Articles by Talal, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meek, K.
Right arrow Articles by Talal, N.

The Journal of Immunology, Vol 144, Issue 4 1375-1381, Copyright © 1990 by American Association of Immunologists

ARTICLES

Anti-peptide antibodies detect a lupus-related interspecies idiotype that maps to H chain CDR2

K Meek, M Takei, H Dang, I Sanz, MJ Dauphinee, JD Capra and N Talal
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.

Antibodies to the small nucleoprotein Sm occur spontaneously in human and murine systemic lupus erythematosus. Human and mouse monoclonal anti-Sm autoantibodies designated 4B4 and Y2 share an idiotype (Id) determinant located on the Ig H chain. To understand the molecular basis of this cross-reactivity, the VH regions of both antibodies were sequenced and analyzed for homology. The antibodies showed only 49.6% homology. The second complementary determining region (CDR2) was the most likely candidate for the Id site. To investigate this possibility, rabbit antiserum was made against a peptide corresponding to the CDR2 of 4B4. This antiserum was specific for the immunizing peptide and reacted weakly to a peptide corresponding to the CDR2 of Y2. Anti-CDR2 antibody bound to 4B4 and Y2 but not to other human and mouse mAb. Binding was directed at the H chain when analyzed by Western blots. Anti-CDR2 antibody blocked anti-Id antibody binding to 4B4 and Y2 by 58% and 24%, respectively. These studies suggest that this interspecies Id maps to the H chain CDR2 and that a conserved Id can occur within molecules that are otherwise radically different.


This article has been cited by other articles:


Home page
 Int ImmunolHome page
H.-L. Wun, D. T.-M. Leung, K.-C. Wong, Y.-L. Chui, and P.-L. Lim
Molecular mimicry: anti-DNA antibodies may arise inadvertently as a response to antibodies generated to microorganisms
Int. Immunol., September 1, 2001; 13(9): 1099 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Hobby, F. J. Ward, A. N. Denbury, D. G. Williams, N. A. Staines, and B. J. Sutton
Molecular Modeling of an Anti-DNA Autoantibody (V-88) and Mapping of Its V Region Epitopes Recognized by Heterologous and Autoimmune Antibodies
J. Immunol., September 15, 1998; 161(6): 2944 - 2952.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
N. A. Staines
Idiotypes of DNA-Binding Antibodies: Recent Advances
Lupus, October 1, 1992; 1(5_suppl): 313 - 316.
[PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.