The JI Acurri Cytometers
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kato, K.
Right arrow Articles by Taniguchi, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kato, K.
Right arrow Articles by Taniguchi, N.

The Journal of Immunology, Vol 144, Issue 4 1317-1322, Copyright © 1990 by American Association of Immunologists

ARTICLES

Detection by in situ hybridization and phenotypic characterization of cells expressing IL-6 mRNA in human stimulated blood

K Kato, T Yokoi, N Takano, H Kanegane, A Yachie, T Miyawaki and N Taniguchi
Department of Pediatrics, School of Medicine, Kanazawa University, Japan.

IL-6 has manifold biologic functions in immune and inflammatory responses and is produced by a variety type of cells. In this work, we used the whole blood culture to identify the cells expressing IL-6 gene/protein after various stimulation. When the whole blood was incubated with LPS or Con A, much IL-6 activity, measured by the growth promoting assay using a murine IL-6-dependent hybridoma clone, was detected in the plasma as early as 4 h of culture and continued to increase with time, reaching a plateau around 12 h. Immunocytochemical analysis with anti-rIL-6 antiserum revealed that a proportion of mononuclear cells (MNC) contained intracytoplasmic IL-6 in LPS- or Con A-stimulated blood. Northern blot analysis for MNC from the blood stimulated with these stimuli showed that their transcripts for IL-6 peaked at 4 h, then rapidly declined and was undetectable after 24 h of stimulation. In situ hybridization technique with radiolabeled antisense RNA probe for IL-6 demonstrated that a fraction of MNC from LPS- as well as Con A-stimulated blood expressed IL-6 mRNA. With the combined use of in situ hybridization and immunofluorescence by corresponding mAb, it was confirmed that IL-6 mRNA expressing cells in stimulated blood were exclusively monocytes. In the whole blood culture, it was shown that expression of IL-6 mRNA by monocytes was inhibited by dexamethasone, but not by cyclosporin A. These results suggest that monocytes are the major cells expressing IL-6 gene/protein in the circulation after exposure to external stimuli.


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
C. M. Paton, J. Brandauer, E. P. Weiss, M. D. Brown, F. M. Ivey, S. M. Roth, and J. M. Hagberg
Hemostatic response to postprandial lipemia before and after exercise training
J Appl Physiol, July 1, 2006; 101(1): 316 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child.Home page
H El Bashir, M Laundy, and R Booy
Diagnosis and treatment of bacterial meningitis
Arch. Dis. Child., July 1, 2003; 88(7): 615 - 620.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Willheim, R. Thien, K. Schrattbauer, E. Bajna, M. Holub, R. Gruber, K. Baier, P. Pietschmann, W. Reinisch, O. Scheiner, et al.
Regulatory Effects of 1{alpha},25-Dihydroxyvitamin D3 on the Cytokine Production of Human Peripheral Blood Lymphocytes
J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3739 - 3744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.