The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Daha, M. R.
Right arrow Articles by van Es, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Daha, M. R.
Right arrow Articles by van Es, L. A.

The Journal of Immunology, Vol 144, Issue 4 1227-1232, Copyright © 1990 by American Association of Immunologists

ARTICLES

Enhanced Ig production by human peripheral lymphocytes induced by aggregated C1q

MR Daha, N Klar, R Hoekzema and LA van Es
Department of Nephrology, University Hospital Leiden, The Netherlands.

Because B cells express receptors for C1q, we have investigated the role of C1q in the stimulation of B cells. When B cells were cultured in the presence of C1q that had been frozen, T cells, and suboptimal concentrations of PWM, there was a dose-dependent enhancement of IgM, IgG, and IgA by the B cells. No significant enhancement of Ig production by B cells was seen in the absence of T cells or PWM. The contribution of T cells or PWM could be replaced by supernatants of PMA and Con A-activated PBMC (T cell growth factor). C1q that had been frozen, in contrast with freshly isolated C1q, was at least 3 times more active in enhancement of the production of Ig by B cells in culture in the presence of suboptimal concentrations of T cell growth factor. The capability of C1q to stimulate B cells could be ascribed to aggregates of C1q. Monomeric C1q was only marginally active to stimulate B cell Ig production, whereas dimeric and tetrameric C1q were able to enhance Ig production by B cells in relation to their size. Furthermore, aggregation of C1q on soluble aggregates of rabbit IgM also increased its potential to enhance B cell Ig production. The interaction of C1q with the B cells occurs via the collagenous tail of C1q, as suggested by inhibition experiments with purified collagenous tails and globular heads of C1q. These results indicate that triggering of C1qR on B cells positively regulates Ig production in vitro.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
E. P. McGreal, N. Ikewaki, H. Akatsu, B. P. Morgan, and P. Gasque
Human C1qRp Is Identical with CD93 and the mNI-11 Antigen But Does Not Bind C1q
J. Immunol., May 15, 2002; 168(10): 5222 - 5232.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. A. KALLIO, K. B. LEMSTRÖM, P. J. HÄYRY, U. S. RYAN, and P. K. KOSKINEN
Blockade of Complement Inhibits Obliterative Bronchiolitis in Rat Tracheal Allografts
Am. J. Respir. Crit. Care Med., April 1, 2000; 161(4): 1332 - 1339.
[Abstract] [Full Text]

Home page
 J. Exp. Med.Home page
A. J. Cutler, M. Botto, D. van Essen, R. Rivi, K. A. Davies, D. Gray, and M. J. Walport
T Cell-dependent Immune Response in C1q-deficient Mice: Defective Interferon gamma  Production by Antigen-specific T Cells
J. Exp. Med., June 1, 1998; 187(11): 1789 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. R. Nepomuceno and A. J. Tenner
C1qRP, the C1q Receptor That Enhances Phagocytosis, Is Detected Specifically in Human Cells of Myeloid Lineage, Endothelial Cells, and Platelets
J. Immunol., February 15, 1998; 160(4): 1929 - 1935.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.