The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cunha-Melo, J. R.
Right arrow Articles by Beaven, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cunha-Melo, J. R.
Right arrow Articles by Beaven, M. A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*12-O-TETRADECANOYLPHORBOL-13-ACETATE

The Journal of Immunology, Vol 143, Issue 8 2617-2625, Copyright © 1989 by American Association of Immunologists

ARTICLES

Studies of protein kinase C in the rat basophilic leukemia (RBL-2H3) cell reveal that antigen-induced signals are not mimicked by the actions of phorbol myristate acetate and Ca2+ ionophore

JR Cunha-Melo, HM Gonzaga, H Ali, FL Huang, KP Huang and MA Beaven
Laboratory of Chemical Pharmacology, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.

Exogenous activators of protein kinase C such as PMA in combination with a Ca2+ ionophore (A23187), cause secretion in rat basophilic (RBL- 2H3) cells,but they do so through stimulatory signals that are not the same as those generated by Ag or oligomers of IgE. On the one hand, the synergy between PMA and A23187 and the suppression of Ag-mediated signals (hydrolysis of inositol phospholipids and rise in concentration of cytosolic Ca2+) by PMA were totally dependent on protein kinase C. The loss of synergistic and inhibitory actions of PMA, for example, correlated with the loss of protein kinase C (as determined by immunoblotting techniques) when cells were continuously exposed to PMA. Furthermore, the permeabilization of RBL-2H3 cells resulted in the loss of both protein kinase C and the inhibitory action of PMA, but both were retained if cells were exposed to PMA before permeabilization Ag- induced secretion, on the other hand, was not as dependent on the presence of protein kinase C. The potent inhibitor of this enzyme, staurosporine, which blocked completely the secretory response to the combination of PMA and A23187, did not inhibit Ag-induced secretion except at concentrations (greater than 10 nM) that inhibited Ag- stimulated hydrolysis of inositol phospholipids as well. Also RBL-2H3 cells still showed some secretory-response (approximately 25% of normal) to Ag when cells were depleted (greater than 98%) of protein kinase C by prolonged treatment with PMA. Previous studies have indicated that the secretory response to PMA and A23187 is much lower than that elicited by Ag when the concentrations of stimulants were matched to give the same increase in concentrations of cytosolic Ca2+.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. P. Morgan, A. Skippen, B. Segui, A. Ball, V. Allen-Baume, B. Larijani, J. Murray-Rust, N. McDonald, G. Sapkota, N. Morrice, et al.
Phosphorylation of a Distinct Structural Form of Phosphatidylinositol Transfer Protein {alpha} at Ser166 by Protein Kinase C Disrupts Receptor-mediated Phospholipase C Signaling by Inhibiting Delivery of Phosphatidylinositol to Membranes
J. Biol. Chem., November 5, 2004; 279(45): 47159 - 47171.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. F. Mahmoud and C. Fewtrell
Microdomains of High Calcium Are Not Required for Exocytosis in Rbl-2h3 Mucosal Mast Cells
J. Cell Biol., April 16, 2001; 153(2): 339 - 350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. I. Ludowyke, J. Holst, L.-M. Mudge, and A. T. R. Sim
Transient Translocation and Activation of Protein Phosphatase 2A during Mast Cell Secretion
J. Biol. Chem., February 25, 2000; 275(9): 6144 - 6152.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. Jost, L. Klickstein, E. Wetzler, A. Kumar, and M. Berger
Intracellular Storage and Regulated Plasma Membrane Expression of Human Complement Receptor Type 1 in Rat Basophil Leukemia Cell Transfectants
Blood, July 1, 1998; 92(1): 300 - 309.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K Xu, R. Williams, D Holowka, and B Baird
Stimulated release of fluorescently labeled IgE fragments that efficiently accumulate in secretory granules after endocytosis in RBL-2H3 mast cells
J. Cell Sci., January 8, 1998; 111(16): 2385 - 2396.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Moriya, J. Rivera, S. Odom, Y. Sakuma, K. Muramato, T. Yoshiuchi, M. Miyamoto, and K. Yamada
ER-27319, an acridone-related compound, inhibits release of antigen-induced allergic mediators from mast cells by selective inhibition of Fcvarepsilon receptor I-mediated activation of Syk
PNAS, November 11, 1997; 94(23): 12539 - 12544.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. C. Wolfe, E.-Y. Chang, J. Rivera, and C. Fewtrell
Differential Effects of the Protein Kinase C Activator Phorbol 12-Myristate 13-Acetate on Calcium Responses and Secretion in Adherent and Suspended RBL-2H3 Mucosal Mast Cells
J. Biol. Chem., March 22, 1996; 271(12): 6658 - 6665.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.