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The Journal of Immunology, Vol 143, Issue 6 1822-1825, Copyright © 1989 by American Association of Immunologists


ARTICLES

Two better cell lines for making hybridomas expressing specific T cell receptors

J White, M Blackman, J Bill, J Kappler, P Marrack, DP Gold and W Born
Howard Hughes Medical Institute, Department of Pediatrics, Denver, CO.

Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be studied at a clonal level, without interference of the BW5147-derived receptor chains. In this study one of the variants has been useful in identifying the reactivity to allogeneic MHC Ag of BW5147 itself.


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